LOCUS       sequence-208853-        8269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID
FEATURES             Location/Qualifiers
     source          1..8269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     54..76
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     68..85
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     69..85
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     92..111
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        92..110
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(144..162)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(614..685,1087..1360,1435..2089,2151..2466,2670..2801,
                     2872..3486)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4261..4294
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4307..4737
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    4798..5228
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5262..5331
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(5550..5568)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5589..5605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5589..5605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5602..5624)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5613..5629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5637..5643,5644..5661,5662..5667))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5682..5703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5820..5837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5991..6579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6071..6090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6750..7541,7542..7610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7373..7392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7611..7715)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7741..8196)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7878..7899)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8090..8109
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
       61 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg
      121 tacgtaccgg gcccctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga
      181 taaaaaaaac attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt
      241 taaatttact tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc
      301 aaaatgacat tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc
      361 gccgtctctt tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag
      421 cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt
      481 ttacgccatc aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt
      541 ttgttttttg agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg
      601 aaaaatcccg gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca
      661 cccatctgcc gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact
      721 tgatctatag gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca
      781 caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat tattctggta
      841 gctgttctcg ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc
      901 attcattttc gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga
      961 tcctagatgc acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta
     1021 ctttttgttc ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca
     1081 ttgcagggtg acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc
     1141 aaaaactacg gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc
     1201 caactagccg agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat
     1261 cagccgggct ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag
     1321 cgacacatac cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc
     1381 ggctgatctg tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg
     1441 gcgtggccta tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga
     1501 ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga
     1561 tgcgactgct caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg
     1621 tccagcagga tgacctcttt atcggctccc taacggccag ggaacacctg attttccaag
     1681 ccatggtgcg gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg
     1741 tgatccagga gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg
     1801 tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gctctaaccg
     1861 atccgccgct tctgatctgc gatgagccca cctccggact ggactccttt accgcccaca
     1921 gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc
     1981 atcagccgtc ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca
     2041 gggtagcttt cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat
     2101 gtgtttatta agggtatcta gtattacata acatctcaac tcctatccag cgtgggtgcc
     2161 cagtgtccta ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc
     2221 ggacgggaga tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgccattagc
     2281 aaagtagccc gggatatgga gcagttgttg gccaccaaaa atctggagaa gccactggag
     2341 cagccggaga atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg
     2401 tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag
     2461 acaacggtga gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa
     2521 attcgctaga ttttagatag aattgcctga ttccacaccc ttcttagttt ttttcaatga
     2581 gatgtatagt ttatagtttt gcagaagata aataaatttc atttaactcg cgaatattaa
     2641 tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt
     2701 tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa cggagccatc ttcctcttcc
     2761 tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtcat gtttagaata
     2821 catttgcatt tcaataattt actaactttc taatgaatcg attcgattta ggtgttcacc
     2881 tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac
     2941 tttctgggca aaacgattgc cgaattgccg ctttttctca cagtgccact ggtcttcacg
     3001 gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg
     3061 gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc
     3121 agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc
     3181 tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac
     3241 ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag
     3301 ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc
     3361 ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt
     3421 ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag
     3481 gagtagccga catatatccg aaataactgc ttgttttttt tttttaccat tattaccatc
     3541 gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa
     3601 caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt
     3661 ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt
     3721 atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc
     3781 tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc
     3841 cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc
     3901 gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt
     3961 aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca
     4021 gctatattta ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg
     4081 gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga
     4141 tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact
     4201 ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa ctaggctagc
     4261 ataacttcgt ataatgtatg ctatacgaag ttatgctagc ggatcctggc cacgtaataa
     4321 gtgtgcgttg aatttattcg caaaaacatt gcatattttc ggcaaagtaa aattttgttg
     4381 cataccttat caaaaaataa gtgctgcata ctttttagag aaaccaaata attttttatt
     4441 gcatacccgt ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac
     4501 tttgacgaaa caaattttcg ttgcataccc aataaaagat tattatattg catacccgtt
     4561 tttaataaaa tacattgcat accctctttt aataaaaaat attgcatacg ttgacgaaac
     4621 aaattttcgt tgcataccca ataaaagatt attatattgc ataccttttc ttgccatacc
     4681 atttagccga tcaattgtgc tcggcaacag tatatttgtg gtgtgccaac caacaacaga
     4741 tctgaattcg cttaattaag cggccgcggc tcgagaaggt accggtctag aagatcttgg
     4801 ccacgtaata agtgtgcgtt gaatttattc gcaaaaacat tgcatatttt cggcaaagta
     4861 aaattttgtt gcatacctta tcaaaaaata agtgctgcat actttttaga gaaaccaaat
     4921 aattttttat tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaaa
     4981 atattgcata ctttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt
     5041 gcatacccgt ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac
     5101 gttgacgaaa caaattttcg ttgcataccc aataaaagat tattatattg catacctttt
     5161 cttgccatac catttagccg atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa
     5221 ccaacaacgg atccactagt gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     5281 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     5341 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     5401 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     5461 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     5521 tcgacactag ttctagccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
     5581 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5641 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5701 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5761 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5821 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5881 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5941 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6001 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6061 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6121 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6181 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6241 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6301 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6361 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6421 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6481 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6541 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6601 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6661 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6721 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6781 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6841 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6901 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6961 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7021 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7081 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7141 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7201 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7261 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7321 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7381 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7441 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7501 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7561 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7621 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7681 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7741 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     7801 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     7861 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     7921 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     7981 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     8041 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     8101 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     8161 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     8221 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagct
//
