LOCUS       sequence-208863-        5721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dLight1.4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Patriarchi T, Cho JR, Merten K, Howe MW, Marley A, Xiong WH, Folk 
            RW, Broussard GJ, Liang R, Jang MJ, Zhong H, Dombeck D, von Zastrow 
            M, Nimmerjahn A, Gradinaru V, Williams JT, Tian L
  TITLE     Ultrafast neuronal imaging of dopamine dynamics with designed 
            genetically encoded sensors.
  JOURNAL   Science. 2018 Jun 29;360(6396). pii: science.aat4422. doi: 
            10.1126/science.aat4422. Epub 2018 May 31.
  PUBMED    29853555
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-06-29"; volume: "360(6396). pii: science.aat4422. doi"; 
            pages: " 10.1126/science.aat4422. Epub 2018 May 31"
COMMENT     Sequence Label: pCMV-dLight1.4
FEATURES             Location/Qualifiers
     source          1..5721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2145..2266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2182..2201)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2236..2255
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2273..2728)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2410..2431)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2622..2641
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2755..2859
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2861..3218
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2861..3052
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2884..2904)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3069..3204
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3253..4047
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3253..4047
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3307..3326)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3917..3936
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4235..4254)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4279..4326
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4655..5243
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5144..5163
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5418..5721
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttggatccg gtacctgcgc gccatgaaga cgatcatcgc
      301 cctgagctac atcttctgcc tggtgttcgc cgactacaag gacgatgatg acgccatggg
      361 gaacagatcc actgcagatg cagacggtct tctcgcaggc cggggacctg ctgccggagc
      421 gagcgctggg gcttccgcag gtcttgctgg gcagggggcc gcggccttgg ttggaggcgt
      481 tttgcttata ggggccgttc ttgctggcaa tagtttggta tgtgtttcag ttgcgacaga
      541 gcgcgcactt cagacgccga ctaactcctt tatagtgagt ttggctgctg cagatctctt
      601 gttggcattg ttggtactcc cactgttcgt ttattcagaa gtacagggtg gcgcatggct
      661 cctgtcaccc aggttgtgtg atgccttgat ggccatggat gttatgctgt gtaccgcttc
      721 tatctttaac ctttgtgcta tcagtgttga cagattcgtc gcggtcgcgg tccctctgag
      781 gtataaccgg caaggaggca gcaggaggca actgctgctg atcggcgcaa cttggctcct
      841 ctccgcagca gtggccgcgc ctgttctgtg tggtctcaac gacgttcgcg gcagagaccc
      901 ggctgtatgt cgcctcgagg atagagatta tgtcgtatac tcaagtgtgt gttccttttt
      961 tcttccttgc ccactgatgc ttctgttgta ttgggctacc tttagaggac tgcaacgcct
     1021 gagctcactc attaatgtat atatcaaagc tgataagcaa aaaaacggta tcaaggctaa
     1081 ttttaagatc agacataata tagaggatgg aggcgttcaa ctggcctacc actaccagca
     1141 aaacacgccg atcggggatg ggccagtact tctgccagat aaccattatc tctcagttca
     1201 aagcaaactc tctaaggacc ctaatgagaa acgagatcat atggttctgc tcgaattcgt
     1261 tacagccgcc ggtatcacac ttgggatgga cgagttgtat aagggtggaa caggagggtc
     1321 aatggtaagc aaaggcgagg agctgtttac gggggtcgtc ccgatacttg ttgaactcga
     1381 cggcgatgtc aacgggcaca aattctcagt gagtggcgag ggggaaggag acgccactta
     1441 tggaaaactg acattgaaat tcatatgtac gactgggaag ttgcctgtgc cttggcctac
     1501 gctcgttact acacttactt acggggtaca gtgtttcagt aggtatccag atcacatgaa
     1561 acagcacgat tttttcaaga gtgcaatgcc ggaaggatat atacaagaaa gaactatttt
     1621 ctttaaagat gacggcaact ataaaacgcg agcagaggtg aagtttgagg gcgatacctt
     1681 ggttaatagg atcgaactca aaggcataga cttcaaagaa gacggaaaca ttctgggtca
     1741 caaactggaa tacaacaatc atgaccaact gggccgcgaa cggaaagcca tgcgagtttt
     1801 gccggtggta gtaggggcat tccttctttg ttggacccct ttttttgtgg tgcatataac
     1861 gcaggctctg tgcccggcct gttctgtccc accccgcctc gtgtcagctg tcacttggtt
     1921 gggttacgta aactcagccc tcaatccagt tatctatacg gttttcaatg ccgagttcag
     1981 gaatgttttt aggaaggccc ttagagcctg ttgttagtgt acagcggccg cgactctaga
     2041 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     2101 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     2161 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2221 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta
     2281 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     2341 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     2401 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     2461 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     2521 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     2581 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     2641 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     2701 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     2761 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     2821 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     2881 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     2941 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     3001 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3061 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3121 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     3181 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     3241 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     3301 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     3361 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     3421 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     3481 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     3541 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     3601 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     3661 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     3721 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     3781 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     3841 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     3901 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     3961 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     4021 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     4081 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     4141 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     4201 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     4261 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     4321 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     4381 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     4441 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     4501 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4561 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     4621 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     4681 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     4741 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4801 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4861 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4921 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4981 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5041 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5101 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5161 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5221 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5281 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5341 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
     5401 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     5461 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     5521 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     5581 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     5641 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     5701 ttagtcatcg ctattaccat g
//
