LOCUS       sequence-208883-        8729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Provides CaMV 35S and 5' part of Tobacco mosaic virus (RdRp 
            polymerase with intron, movement protein and sgCP promoter) as level
            0 GoldenBraid part (GGAG-AATG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8729)
  TITLE     Moravec lab plasmids
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2:35S-TMV-Rep-MP
FEATURES             Location/Qualifiers
     source          1..8729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      34..91
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(115..132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(286..874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(366..385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1057..1151)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1172..1831)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1746..1765
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1993..2011
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2015..2058)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2061..2082
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        2160..2505
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     2448..2468
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    2514..2569
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
ORIGIN
        1 accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc
       61 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
      121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
      301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
      481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
      541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
      601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
      661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
      721 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
      781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
      841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
      901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
      961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1021 atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg
     1081 gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat
     1141 caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt
     1201 actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa
     1261 cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga
     1321 aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca
     1381 cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca
     1441 ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt
     1501 cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac
     1561 aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata cgaaattccg
     1621 gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat
     1681 ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac
     1741 attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa
     1801 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
     1861 ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
     1921 ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat tatcatgaca
     1981 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
     2041 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt ggtctcggga
     2101 gacacgcttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt
     2161 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc
     2221 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc
     2281 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc
     2341 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg
     2401 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa
     2461 gacccttcct ctatataagg aagttcattt catttggaga gggtattttt acaacaatta
     2521 ccaacaacaa caaacaacaa acaacattac aattactatt tacaattaca atggcataca
     2581 cacagacagc taccacatca gctttgctgg acactgtccg aggaaacaac tccttggtca
     2641 atgatctagc aaagcgtcgt ctttacgaca cagcggttga agagtttaac gctcgtgacc
     2701 gcaggcccaa ggtgaacttt tcaaaagtaa taagcgagga gcagacgctt attgctaccc
     2761 gggcgtatcc agaattccaa attacatttt ataacacgca aaatgccgtg cattcgcttg
     2821 caggtaataa cctgatcctt agaacttttt ttaacctatg tttccgtgca tatttatgtg
     2881 tcttgaagaa ctacatcacc ttacggacat aaataatata tactgacagt gtaaagatct
     2941 tttaccatat cagtacaatt taacatgtgc ataattgtag attagttact atttttactc
     3001 ttatagtaag ttatatatag tgacaatgta aggatctttt accatgttag tgcagtttaa
     3061 catgtgcatg attgtaggtc agttaccatt tttattattc tagtggcgag tataacctac
     3121 tatgatagta agtcaagttc atttactttg atgtgataaa aattaagtta gtttaacgtg
     3181 tcagaataat tctttgactt tactgctata gtggataaag ttcagttatg tggttacttt
     3241 tgtgacttta ccgttatagt gtgagaaaaa gttgagtgac caaacgtgaa tttaacagtg
     3301 tatatttgca caggaggatt gcgatcttta gaactggaat atctgatgat gcaaattccc
     3361 tacggatcat tgacttatga cataggcggg aattttgcat ctcatctgtt caagggacga
     3421 gcatatgtac actgctgcat gcccaacctg gacgttcgag acatcatgcg gcacgaaggc
     3481 cagaaagaca gtattgaact atacctttct aggctagaga gaggggggaa aacagtcccc
     3541 aacttccaaa aggaagcatt tgacagatac gcagaaattc ctgaagacgc tgtctgtcac
     3601 aatactttcc agacatgcga acatcagccg atgcagcaat caggcagagt gtatgccatt
     3661 gcgctacaca gcatatatga cataccagcc gatgagttcg gggcggcact cttgaggaaa
     3721 aatgtccata cgtgctatgc cgctttccac ttctctgaga acctgcttct tgaagattca
     3781 tacgtcaatt tggacgaaat caacgcgtgt ttttcgcgcg atgaagacaa gttgaccttt
     3841 tcttttgcat cagagagtac tcttaattat tgtcatagtt attctaatat tcttaagtat
     3901 gtgtgcaaaa cttacttccc ggcctctaat agagaggttt acatgaagga gtttttagtc
     3961 accagagtta atacctggtt ttgtaagttt tctagaatag atacttttct tttgtacaaa
     4021 ggtgtggccc ataaaagtgt agatagtgag cagttttata ctgcaatgga agacgcatgg
     4081 cattacaaaa agactcttgc aatgtgcaac agcgagagaa tcctccttga ggattcatca
     4141 tcagtcaatt actggtttcc caaaatgagg gatatggtca tcgtaccatt attcgacatt
     4201 tctttggaga ctagtaagag gacgcgcaag gaagtcttag tgtccaagga tttcgtgttt
     4261 acagtgctta accacattcg aacataccag gcgaaagctc ttacatacgc aaatgttttg
     4321 tcctttgtcg aatcgattcg atcgagggta atcattaacg gtgtgacagc gaggtccgaa
     4381 tgggatgtgg acaaatcttt gttacaatcc ttgtccatga cgttttacct gcatactaag
     4441 cttgccgttc taaaggatga cttactgatt agcaagttta gtctcggttc gaaaacggtg
     4501 tgccagcatg tgtgggatga gatttcgctg gcgtttggga acgcatttcc ctccgtgaaa
     4561 gagaggctct tgaacaggaa acttatcaga gtggcaggcg acgcattaga gatcagggtg
     4621 cctgatctat atgtgacctt ccacgacaga ttagtgactg agtacaaggc ctctgttgac
     4681 atgcctgcgc ttgacattag gaagaagatg gaagaaacgg aagtgatgta caatgcactt
     4741 tcagagttat cggtgttaag ggagtctgac aaattcgatg ttgatgtttt ttcccagatg
     4801 tgccaatctt tggaagttga cccaatgacg gcagcgaagg ttatagtcgc ggtcatgagc
     4861 aatgagagcg gtctgactct cacatttgaa cgacctactg aggcgaatgt tgcgctagct
     4921 ttacaggatc aagagaaggc ttcagaaggt gctttggtag ttacctcaag agaagttgaa
     4981 gaaccgtcca tgaagggttc gatggccaga ggagagttac aattagctgg tcttgctgga
     5041 gatcatccgg agtcgtccta ttctaagaac gaggagatag agtctttaga gcagtttcat
     5101 atggcaacgg cagattcgtt aattcgtaag cagattagct cgattgtgta cacgggtccg
     5161 attaaagttc agcaaatgaa aaactttatc gatagcctgg tagcatcact atctgctgcg
     5221 gtgtcgaatc tcgtcaagat cctcaaagat acagctgcta ttgaccttga aacccgtcaa
     5281 aagtttggag tcttggatgt tgcatctagg aagtggttaa tcaaaccaac ggccaagagt
     5341 catgcatggg gtgttgttga aacccacgcg aggaagtatc atgtggcgct tttggaatat
     5401 gatgagcagg gtgtggtgac atgcgacgat tggagaagag tagctgtcag ctctgagtct
     5461 gttgtttatt ccgacatggc gaaactcaga actctgcgca gactgcttcg aaacggagaa
     5521 ccgcatgtca gtagcgcaaa ggttgttctt gtggacggag ttccgggctg tggaaaaacc
     5581 aaagaaattc tttccagggt taattttgat gaagatctaa ttttagtacc tgggaagcaa
     5641 gccgcggaaa tgatcagaag acgtgcgaat tcctcaggga ttattgtggc cacgaaggac
     5701 aacgttaaaa ccgttgattc tttcatgatg aattttggga aaagcacacg ctgtcagttc
     5761 aagaggttat tcattgatga agggttgatg ttgcatactg gttgtgttaa ttttcttgtg
     5821 gcgatgtcat tgtgcgaaat tgcatatgtt tacggagaca cacagcagat tccatacatc
     5881 aatagagttt caggattccc gtaccccgcc cattttgcca aattggaagt tgacgaggtg
     5941 gagacacgca gaactactct ccgttgtcca gccgatgtca cacattatct gaacaggaga
     6001 tatgagggct ttgtcatgag cacttcttcg gttaaaaagt ctgtttcgca ggagatggtc
     6061 ggcggagccg ccgtgatcaa tccgatctca aaacccttgc atggcaagat cctgactttt
     6121 acccaatcgg ataaagaagc tctgctttca agagggtatt cagatgttca cactgtgcat
     6181 gaagtgcaag gcgagacata ctctgatgtt tcactagtta ggttaacccc tacaccagtc
     6241 tccatcattg caggagacag cccacatgtt ttggtcgcat tgtcaaggca cacctgttcg
     6301 ctcaagtact acactgttgt tatggatcct ttagttagta tcattagaga tctagagaaa
     6361 cttagctcgt acttgttaga tatgtataag gttgatgcag gaacacaata gcaattacag
     6421 attgactcgg tgttcaaagg ttccaatctt tttgttgcag cgccaaagac tggtgatatt
     6481 tctgatatgc agttttacta tgataagtgt ctcccaggca acagcaccat gatgaataat
     6541 tttgatgctg ttaccatgag gttgactgac atttcattga atgtcaaaga ttgcatattg
     6601 gatatgtcta agtctgttgc tgcgcctaag gatcaaatca aaccactaat acctatggta
     6661 cgaacggcgg cagaaatgcc acgccagact ggactattgg aaaatttagt ggcgatgatt
     6721 aaaaggaact ttaacgcacc cgagttgtct ggcatcattg atattgaaaa tactgcatct
     6781 ttggttgtag ataagttttt tgatagttat ttgcttaaag aaaaaagaaa accaaataaa
     6841 aatgtttctt tgttcagtag agagtctctc aatagatggt tagaaaagca ggaacaggta
     6901 acaataggcc agctcgcaga ttttgatttt gtggatttgc cagcagttga tcagtacaga
     6961 cacatgatta aagcacaacc caagcaaaag ttggacactt caatccaaac ggagtacccg
     7021 gctttgcaga cgattgtgta ccattcaaaa aagatcaatg caatattcgg cccgttgttt
     7081 agtgagctta ctaggcaatt actggacagt gttgattcga gcagattttt gtttttcaca
     7141 agaaagacac cagcgcagat tgaggatttc ttcggagatc tcgacagtca tgtgccgatg
     7201 gatgtcttgg agctggatat atcaaaatac gacaaatctc agaatgaatt ccactgtgca
     7261 gtagaatacg agatctggcg aagattgggt ttcgaagact tcttgggaga agtttggaaa
     7321 caagggcata gaaagaccac cctcaaggat tataccgcag gtataaaaac ttgcatctgg
     7381 tatcaaagaa agagcgggga cgtcacgacg ttcattggaa acactgtgat cattgctgca
     7441 tgtttggcct cgatgcttcc gatggagaaa ataatcaaag gagccttttg cggtgacgat
     7501 agtctgctgt actttccaaa gggttgtgag tttccggatg tgcaacactc cgcgaatctt
     7561 atgtggaatt ttgaagcaaa actgtttaaa aaacagtatg gatacttttg cggaagatat
     7621 gtaatacatc acgacagagg atgcattgtg tattacgatc ccctaaagtt gatctcgaaa
     7681 cttggtgcta aacacatcaa ggattgggaa cacttggagg agttcagaag atctctttgt
     7741 gatgttgctg tttcgttgaa caattgtgcg tattacacac agttggacga cgctgtatgg
     7801 gaggttcata agaccgcccc tccaggttcg tttgtttata aaagtctggt gaagtatttg
     7861 tctgataaag ttctttttag aagtttgttt atagatggct ctagttgtta aaggaaaagt
     7921 gaatatcaat gagtttatcg acctgacaaa aatggagaag atcttaccgt cgatgtttac
     7981 ccctgtaaag agtgttatgt gttccaaagt tgataaaata atggttcatg agaatgagtc
     8041 attgtcaggg gtgaaccttc ttaaaggagt taagcttatt gatagtggat acgtctgttt
     8101 agccggtttg gtcgtcacgg gcgagtggaa cttgcctgac aattgcagag gaggtgtgag
     8161 cgtgtgtctg gtggacaaaa ggatggaaag agccgacgag gccactctcg gatcttacta
     8221 cacagcagct gcaaagaaaa gatttcagtt caaggtcgtt cccaattatg ctataaccac
     8281 ccaggacgcg atgaaaaacg tctggcaagt tttagttaat attagaaatg tgaagatgtc
     8341 agcgggtttc tgtccgcttt ctctggagtt tgtgtcggtg tgtattgttt atagaaataa
     8401 tataaaatta ggtttgagag agaagattac aaacgtgagg gacggagggc ccatggaact
     8461 tacagaagaa gtcgttgatg agttcatgga agatgtccct atgtcgatca ggcttgcaaa
     8521 gtttcgatct cgaaccggaa aaaagagtga tgtccgcaaa gggaaaaata gtagtaacga
     8581 tcggtcagtg ccgaacaaga actatagaaa tgttaaggat tttggaggaa tgagttttaa
     8641 aaagaataat ttaatcgatg atgattcgga ggctactgtc gccgaatcgg attcgtttta
     8701 aatacgtctt acgatctaaa gaatgtgag
//
