LOCUS       sequence-208889-       10303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-OTUD4
FEATURES             Location/Qualifiers
     source          1..10303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             177..693
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    757..1098
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1165..1581
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1511..1533
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1549..1565
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1596..1975
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1976..2179
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2130..2150
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2181..2199
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2202..2220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2230..2254
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2327..2336
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2336..2359
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2372..2398
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2414..2438
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4483..4500
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    complement(5586..5610)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5629..6128
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5664..5687)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6061..6080
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6149..6748
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6149..6168)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6645..6665
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6874..7388
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    7550..7566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7574..7580,7581..7598,7599..7604))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7619..7640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7757..7774)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7928..8516)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8008..8027)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8687..9478,9479..9547))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9310..9329
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9548..9652)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9720..9738
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9776..9798)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9898..9917
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10111..10133
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10261..10280
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ccggtgatgc cggccacgat gcgtccggcg tagaggcgat tagtccaatt tgttaaagac
       61 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
      121 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
      181 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
      241 aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga
      301 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
      361 agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc
      421 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
      481 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
      541 cactcggcgc gccagtcctc cgatagactg cgtcgcccgg gtacccgtat tcccaataaa
      601 gcctcttgct gtttgcatcc gaatcgtgga ctcgctgatc cttgggaggg tctcctcaga
      661 ttgattgact gcccacctcg ggggtctttc atttggaggt tccaccgaga tttggagacc
      721 cctgcccagg gaccaccgac ccccccgccg ggaggtaagc tggccagcgg tcgtttcgtg
      781 tctgtctctg tctttgtgcg tgtttgtgcc ggcatctaat gtttgcgcct gcgtctgtac
      841 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttctgaaca
      901 cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg tggcccgacc
      961 tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct ggtaggagac
     1021 gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg gaaccgaagc
     1081 cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg tctgactgtg
     1141 tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag tttgacctta
     1201 ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga
     1261 cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac
     1321 ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc acctggcccg
     1381 catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc
     1441 cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc
     1501 ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc
     1561 ctcactcctt ctctaggcgc cggaattaga tccttgacat tgattattga ctagttatta
     1621 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     1681 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     1741 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     1801 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     1861 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     1921 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     1981 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     2041 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     2101 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     2161 ggtctatata agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga
     2221 tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     2281 tgacctccat agaagacacc gggaccgatc cagcctccgg ggatctgcca ccatggacta
     2341 caaggatgac gatgacaagc tcgatggagg atacccatac gatgttccag attacgctgc
     2401 tcgaggttat caaacaagtt tgtacaaaaa agttggcatg gcctgtattc actatcttcg
     2461 agagaacaga gagaaatttg aagcgtttat agaaggatca tttgaagaat atttaaagcg
     2521 tttggaaaat ccacaggaat gggtaggaca agtggaaata agtgcccttt ctcttatgta
     2581 caggaaagat tttataattt atcgggaacc aaatgtttct ccttcacaag taacagaaaa
     2641 taattttcct gaaaaggtgt tactgtgttt ttcaaatgga aatcattatg atattgtgta
     2701 tcccataaag tataaagaaa gctctgctat gtgtcagtct ctcctttatg aattgctgta
     2761 tgagaaggta tttaaaactg atgttagtaa aattgtgatg gaactagaca cgttggaagt
     2821 agctgatgaa gataacagtg aaatatcaga ttcagaggat gacagttgca agagtaagac
     2881 tgctgctgct gctgctgatg tgaatggatt taaacctttg tcaggcaatg agcagctgaa
     2941 gaacaatggg aactctacta gcctgccttt gtctagaaag gttcttaagt cactcaatcc
     3001 tgcagtctat agaaatgtgg aatatgaaat ttggctgaag tctaaacaag ctcagcaaaa
     3061 acgtgattat tccattgctg ctggcttaca atatgaagtt ggagacaaat gtcaagttag
     3121 gttggatcac aatggaaaat ttttgaatgc agatgttcaa ggaattcatt ctgagaatgg
     3181 accagttttg gttgaagaac tgggaaagaa gcacacatca aagaacctca aggcacctcc
     3241 cccagaaagc tggaacacag tgtcagggaa gaagatgaaa aaaccttcca cttctggaca
     3301 aaatttccat tctgatgtgg attacagagg gccaaagaat ccaagcaagc caataaaagc
     3361 cccatcagca ctacctcctc gactgcagca tccttcagga gtaagacaac atgcgttctc
     3421 tagtcattct tcagggtcac agtctcagaa attctccagt gagcacaaaa atcttagccg
     3481 gacaccttca cagatcataa gaaaacctga tcgtgaaaga gttgaggatt ttgatcacac
     3541 aagtcgagaa tctaactatt tcggcctttc cccagaagag cgcagagaga agcaagctat
     3601 agaagaatcc cgtttactct atgagattca gaacagagat gaacaggctt tcccagccct
     3661 ttccagctca tcagtcaatc agtcagcttc tcagagtagc aatccatgtg tccagagaaa
     3721 atcatcacat gtaggtgata gaaaaggaag caggcggaga atggatacag aagaacgaaa
     3781 agacaaagac tctattcatg gacatagtca gttggataaa agacccgaac caagcacatt
     3841 ggagaatatt actgatgata aatatgcaac agtttcatca ccatcaaagt caaagaagtt
     3901 agagtgccct tctcctgcgg aacaaaagcc agcagaacat gtgtctttgt caaatccagc
     3961 tccccttcta gtttctccag aggtacatct aactcctgcg gtgccttctt taccagccac
     4021 tgtgccagcc tggccaagtg aacctacaac ttttggacca acaggtgtcc ctgctccaat
     4081 tcccgttttg tcagtgacac agactttgac cactggacct gattcagctg tatcccaagc
     4141 tcatttaaca ccctctccag ttcctgtgtc aatacaggca gttaaccagc ccttgatgcc
     4201 tttgcctcag acattgagcc tttatcaaga cccactctat cctgggtttc cttgtaatga
     4261 aaagggagat cgagccattg taccacctta ttcactgtgt cagactgggg aggacctacc
     4321 taaagataag aatattcttc gattcttctt caatcttggt gtgaaggcat acagttgtcc
     4381 tatgtgggcc ccacattctt acctgtaccc tctgcaccag gcctacctgg cagcctgcag
     4441 gatgtaccca aaggtccctg tccctgttta tcctcataat ccctggttcc aagaggctcc
     4501 tgctgctcag aatgaaagtg attgtacctg tactgatgcc cactttccta tgcagactga
     4561 ggccagtgtt aatggtcaaa tgccacagcc agagattgga ccgccgacat tttcttcacc
     4621 tctggttatc cctccatctc aggtgtctga aagtcatgga caattgtctt accaggctga
     4681 tcttgaatct gagacccctg ggcagcttct gcatgctgat tatgaagagt cactaagtgg
     4741 caagaatatg ttcccccagc catcttttgg acccaatcca ttcttaggcc cagttcctat
     4801 tgcacctcct ttctttcctc atgtttggta tgggtaccct tttcagggat tcatagaaaa
     4861 tccagtaatg aggcagaata ttgtcctgcc ctctgatgaa aaaggagaat tggatctgtc
     4921 tctggaaaat ctggatctgt ctaaagattg tggttcagtt tcaacagtag atgagtttcc
     4981 agaagccagg ggtgaacatg tacattctct ccctgaagca agtgtgagca gtaagccgga
     5041 cgaaggccgg acagagcaat cttcccagac acgaaaggca gatacggcat tggcttccat
     5101 ccctcctgta gcagagggaa aggctcatcc tcccactcag attctaaaca gagagagaga
     5161 aactgtgcct gttgaacttg aacctaaaag gaccattcaa agcctgaaag aaaaaacaga
     5221 aaaagtaaaa gatcctaaga ctgctgctga tgtggtcagc cctggggcca actctgttga
     5281 tagcagagtg caaagaccaa aagaagagag ttcagaagat gaaaatgaag tgtctaatat
     5341 tttgagaagt ggtagatcca agcagttcta taatcaaact tatggaagca ggaagtacaa
     5401 aagtgattgg ggctattctg gtaggggtgg atatcaacat gtgagaagtg aggagtcctg
     5461 gaaaggacag ccaagcagaa gtcgggatga aggttatcag taccatcgaa atgtcagagg
     5521 gcgaccattt aggggagata ggaggagatc agggatggga gatggccata ggggacagca
     5581 cacttgacca actttcttgt acaaagtggt tcgataacga attctaccgg gtaggggagg
     5641 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     5701 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     5761 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     5821 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     5881 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     5941 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     6001 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     6061 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     6121 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     6181 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6241 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6301 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6361 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6421 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6481 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6541 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6601 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     6661 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6721 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     6781 aaggagcgca cgaccccatg catcgataaa ataaaagatt ttatttagtc tccagaaaaa
     6841 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     6901 aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga
     6961 agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt
     7021 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg
     7081 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     7141 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     7201 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     7261 agactgcgtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     7321 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     7381 tctttcatgg gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa
     7441 tattaagtaa tcctgactca attagccact gttttgaatc cacatactcc aatactcctg
     7501 aaatagttca ttatggacag cgcagaaaga gctggggaga attgtgaaat tgttatccgc
     7561 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7621 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7681 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7741 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7801 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7861 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7921 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7981 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8041 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8101 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8161 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8221 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8281 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8341 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8401 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8461 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8521 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8581 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8641 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8701 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8761 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8821 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8881 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8941 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9001 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9061 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9121 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9181 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9241 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9301 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9361 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9421 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9481 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9541 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9601 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9661 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9721 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     9781 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     9841 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9901 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9961 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
    10021 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10081 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcgc
    10141 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
    10201 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
    10261 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcg
//
