LOCUS       sequence-208920-       10302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of MET fusion protein in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10302)
  AUTHORS   Reeves TD, Warner DM, Ludlow LH, O'Connor CM
  TITLE     Pathways over Time: Functional Genomics Research in an Introductory 
            Laboratory Course.
  JOURNAL   CBE Life Sci Educ. 2018 Spring;17(1). pii: 17/1/ar1. doi: 
            10.1187/cbe.17-01-0012.
  PUBMED    29326101
REFERENCE   2  (bases 1 to 10302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "CBE Life 
            Sci Educ."; date: "2018-?"; volume: "17(1). pii: 17/1/ar1. doi"; 
            pages: " 10.1187/cbe.17-01-0012"
COMMENT     Sequence Label: pYES2.1-pomSir1
FEATURES             Location/Qualifiers
     source          1..10302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..442
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    1..118
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     413..436
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     474..493
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        474..492
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4961..5002
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5012..5029
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5050..5297
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5066..5084)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5374..5391)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5545..6133)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5625..5644)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6304..7095,7096..7164))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6927..6946
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(7260..8063)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8064..8279)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     rep_origin      8880..9757
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     rep_origin      complement(9768..10281)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9963..9984)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10175..10194
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
       61 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
      121 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
      181 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
      241 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
      301 aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg
      361 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc
      421 tctatacttt aacgtcaagg agaaaaaacc ccggatcgga ctactagcag ctgtaatacg
      481 actcactata gggaatatta agctcgccct tatgagtgta aaggctagta tcaataattc
      541 tcaggaggct gtctcacgca tcgctttccg ttgctcagat gcgttgtacg tggttcatcc
      601 taacaattcg attttaaatg gtgctttaac agaatcctta aaagatttaa aaaaatttga
      661 aacgttaaat gtttccggca aggttccaca cgttcttcct ttaaaatctc acgctgatcc
      721 ctttgctcac attgcggatg ctattttggc tgaagaagaa gtggcttcta ctcaacctaa
      781 acaaatcaca tctgtagttg cttctgctga tgcactcttt ttcgctactc ctcatcttta
      841 taagttggct catgagcctc ttgtagcgca cgttgccata gagtctacag aggcatttga
      901 ttttgcttct gtgcgtgaca ccggtttcgt tattctcttt tcgggcaatc gccctggtga
      961 ttcttcggag gctgctttgg aagatacctt ggagacagct tctttggctc atcgcttagc
     1021 attgaaattg aacacaggcg ttttacattt ctattctcca gtttacgaca cgaccgctgc
     1081 gttggagaac atcgagacac taccttccaa agaggatgct caacacgcta gagttgccca
     1141 cattcctatt gaagagaaac aggaggattc tgaaaaagaa ggcaatatca aggaagcttt
     1201 tgtccctccc aagtttgatc agccagaacg tgatgccgct acttctgaat accttgaatc
     1261 attgtctatc aagccttttg aatactctgg ttccgatgac gctactgatg tcttgttagt
     1321 tttcggaagc gctgcttctg aattagccaa ggctgccgtt acttcctctg tggcagttgc
     1381 tattgtaaga gtccttcgcc cttggttgcc ctctaaattg caagaagtgc ttcctacttc
     1441 tactaagcgc ttgactgttt tggaacccat taccagtctt cctcgtaaat gggatccgct
     1501 ttaccttgac gttttatctt ctttcgtcgc ttccggatct tcaattgagc tgtttgccgt
     1561 aagatacgga ttgagttctt cagagcaagc cacagagatt attaaagctg ttcgtgataa
     1621 tctttcaggc gctttaaagc catctctcgt ttgcgatttt accgatggtg tttctcaagt
     1681 gtttgttcct acacctccgt ctattgagga agcttatcat aaacttcttc atagagtctt
     1741 taagtctcgt cttaacattg ttaacgatcc cgcttcatca gctacgaaac aaaacattcc
     1801 atctcgtctc atcatatctc ctcagtttgc tttgggttcg gttttagagt atgaaaatca
     1861 gcgtcgcgct ttctgtgatg aggttgctac tttattgaaa gaaaagaatt cttctgtctc
     1921 ttctgaatca ttagaagttc tttcgaattg gattgtttcc gttgataacc tggagtctcc
     1981 tgttgaccct gagttggtca tctctgaact taagaaggat tcatctgctc ctatcaaatc
     2041 acttttagat cgatctgaat tttttaccaa cgtttcacgt tggattatcg gttccgatgc
     2101 ttgggcatat gacttgggta attctgctct tcatcaagta ctttgcttgg aaaagaacgt
     2161 caatttattg attgttgaca cccagcctta ctctactcgt gaagccgtcc gatcctcttc
     2221 cagaaagaag gatattggat tgtatgccat gaactttggc aatgcatatg tcgcctctac
     2281 tgcgttgtat agttcataca cccaactaat ctcggcactt ttagaagcag ataaatttaa
     2341 gggcccctcg gttgttttgg cataccttcc ctatcacagt gctgacgatg atgccattac
     2401 tgtgttacag gaaaccaaga aagccgttga catcggttat tggcctttgt atagatggac
     2461 tcctgcttta gaggacggag aatattcaga tttcaaactt gattccgaac gtattcgccg
     2521 agagctcaag acctttttgg aaagggataa ctacttgact caactaacgc ttcgtgtgcc
     2581 aagtttagct cgtactctta ctcaatcatt tggtgcggaa gtccgtcatc aacaaaacgt
     2641 tgactcacgc aatgctctca acaaattaat tgaaggtttg tctggtcctc ctcttaccat
     2701 tcttttcgct tcagacggag gtacagctga aaacgttgcg aagagacttc aaaaccggac
     2761 ttctgcaaga ggatccaagt gtaagattat ggccatggat gatttcccta tcgaggagtt
     2821 aggcaatgag aagaatgtcg tagtcttggt tagtacagct ggtcaaggtg agtttcctca
     2881 aaatggacgt gagttttggg aagcaatcaa aggtgcagat cttaacttga gcgaattgaa
     2941 gtatggtgta tttggttttg gcgattatga atattggcct cgtaaggaag ataaaattta
     3001 ttataaccgc ccaggtaagc aattagatgc tcgctttgtt gaattgggtg ctgctccatt
     3061 ggtaacctta ggacttggta atgaccagga tccagatgga tgggaaacag catataacct
     3121 ttgggagcct gagctttgga aagctcttgg attggacaat gtcgaaattg atattgatga
     3181 acctaagcct atcactaatg aagacattaa acaagcttcg aactttttgc gtggtaccat
     3241 ttttgaagga ttggccgatg aaagtactgg tgctttggcc gaaagtgact gtcaacttac
     3301 caaattccat ggtatttata tgcaagatga tcgtgacatt cgtgatgagc gtaaaaaaca
     3361 aggtttggaa cctgcttatg gtttcatgat tcgcgccaga atgcctgctg gtgtctgtac
     3421 accagaacaa tggattgcta tggatgatat ttctacgaaa tggggtaatc acacccttaa
     3481 gattacgact cgtcaaacgt tccaatggca tggtgttttg aagaagaatt tgcgtaatac
     3541 cattcgtaac atcgttaaag ttttccttac tactcttggt gcttgtggtg atgttgctcg
     3601 taatgtcact tgttcgtcga ctcccaacaa tgaaacaatt cacgatcaac tgtttgctgt
     3661 atctaagcaa atcagtaatg aattgcttcc tactacatcc tcttatcatg aaatttggat
     3721 cgaggacccc gaaactgtcg aaaagagaaa ggttgccggt gaggccgttc aggatgtcga
     3781 gcctttatat ggtcctacat atctgcccag aaaattcaag gttggtgtgg ctgctccccc
     3841 ttacaatgat gtcgatgttt ataccaatga tgttgcttta atcgccatca ttgaaaatga
     3901 caaagtccta ggtttcaatg ttggtattgg tggtggtatg ggcacaaccc ataacaacaa
     3961 gaaaacatat cctcgtttgg ctacagttgt tggctatgtt ctaactgata agattatgga
     4021 ggtggttaaa gctattttga ttgtccagcg tgataacggt gatcgtgaaa atcgtaagca
     4081 cgctcgtctt aagtataccg ttgatactct tggtgtttct accttcgtcg agaaggtcga
     4141 ggaggttttg ggttacaaat ttgaagaggc acgtgatcat ccccaattca ttaaaaacca
     4201 cgatgacttc gaaggctggc acaagacaga gaaaaacaaa tactggcgtt ctatatttgt
     4261 tgaaaacggt cgtattgaga acaatggtat tttacagttt aagaccggtt tgcgtgaact
     4321 cgcagaacgt ctatatactg aaaaatctga ggctgaattc cgtttgactg ccaaccagca
     4381 tgtcatctta tttaatgttg ctgaaaatga gctcggttgg attaatgagc atatggccaa
     4441 atataaactt gataataatg cattctctgg cttacgtctt tcttctgcag cctgcgttgc
     4501 tctccctact tgtggtttag ccatggctga aagtgaacgt tatcttccca agttgattac
     4561 gaaagtagaa gaaattgttt acgaagctgg tcttcagaaa gatagtattg ttatgcgtat
     4621 gactggttgt ccaaatggat gctctcgtcc atgggttgct gaaattgcgt gtgtcggtaa
     4681 ggcaccaaat acttacaatc taatgcttgg tggaggattt tacggtcaga gattaaacaa
     4741 gctatatcgc tcttccgttc aagaaaaaga aatcctcaat ttgttgcgtc cgcttatcaa
     4801 gcgttacgcc ttggagcgtg aagatggtga acactttggt gattgggtta ttcgtgcagg
     4861 tatcattaca gctgttgaaa atggaggagc taatggtgct gttcatgaag gggtatcacc
     4921 tgaagcgttt aagggcgagc ttcgaggtca cccattcgaa ggtaagccta tccctaaccc
     4981 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttctag
     5041 agggccgcat catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg
     5101 ctctaaccga aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta
     5161 tagttatgtt agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca
     5221 gacgcgtgta cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct
     5281 cgaaggcttt aatttgcaag ctgcggccct gcattaatga atcggccaac gcgcggggag
     5341 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5521 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5641 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6241 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6361 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6601 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6901 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tgggtaataa ctgatataat
     7201 taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt
     7261 tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca
     7321 ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca
     7381 gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg
     7441 tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc
     7501 atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca
     7561 gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc
     7621 aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata
     7681 cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca
     7741 cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag
     7801 agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa
     7861 aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca
     7921 actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc
     7981 ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt
     8041 tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc
     8101 agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat
     8161 accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca
     8221 aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa
     8281 agctagctta tcgatgataa gctgtcaaac atgagaatta attccacgga ctatagacta
     8341 tacctagtat actccgtcta ctgtacgata cacttccgct caggtccttg tcctttaacg
     8401 aggccttacc actcttttgt tactctattg atccagctca gcaaaggcag tgtgatctaa
     8461 gattctatct tcgcgatgta gtaaaactag ctagaccgag aaagagacta gaaatgcaaa
     8521 aggcacttct acaatggctg ccatcattat tatccgatgt gacgctgcag cttctcaatg
     8581 atattcgaat acgctttgag gagatacagc ctaatatccg acaaactgtt ttacagattt
     8641 acgatcgtac ttgttaccca tcattgaatt ttgaacatcc gaacctggga gttttccctg
     8701 aaacagatag tatatttgaa cctgtataat aatatatagt ctagcgcttt acggaagaca
     8761 atgtatgtat ttcggttcct ggagaaacta ttgcatctat tgcataggta atcttgcacg
     8821 tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact tcaatagcat atctttgtta
     8881 acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc
     8941 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt
     9001 accaacgaag aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat
     9061 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgagagcgct
     9121 attttaccaa caaagaatct atacttcttt tttgttctac aaaaatgcat cccgagagcg
     9181 ctatttttct aacaaagcat cttagattac tttttttctc ctttgtgcgc tctataatgc
     9241 agtctcttga taactttttg cactgtaggt ccgttaaggt tagaagaagg ctactttggt
     9301 gtctattttc tcttccataa aaaaagcctg actccacttc ccgcgtttac tgattactag
     9361 cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc gattatattc tataccgatg
     9421 tggattgcgc atactttgtg aacagaaagt gatagcgttg atgattcttc attggtcaga
     9481 aaattatgaa cggtttcttc tattttgtct ctatatacta cgtataggaa atgtttacat
     9541 tttcgtattg ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag
     9601 agtaatacta gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga
     9661 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
     9721 acttttgagc aatgtttgtg gaagcggtat tcgcaatggg aagctccacc ccggttgata
     9781 atcagaaaag ccccaaaaac aggaagattg tataagcaaa tatttaaatt gtaagcgtta
     9841 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     9901 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     9961 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
    10021 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
    10081 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
    10141 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
    10201 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
    10261 atgcgccgct acagggcgcg tggggatcga tccactagtc ca
//
