LOCUS       sequence-208930-        2979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the insect cell expression system, donor for plasmid 
            fusions, two promoters, Multiplication Module M, LoxP site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, 
            Berger I
  TITLE     Protein complex expression by using multigene baculoviral vectors.
  JOURNAL   Nat Methods. 2006 Dec;3(12):1021-32. doi: 10.1038/nmeth983.
  PUBMED    17117155
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-12"; volume: "3"; issue: "12"; pages: 
            "1021-32"
COMMENT     Sequence Label: pUCDM
FEATURES             Location/Qualifiers
     source          1..2979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..362
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(415..448)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    638..686
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     711..730
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(822..931)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        976..1067
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     992..1009
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1326..1460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1363..1382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1417..1436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        1831..1933
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1934..2593
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2000..2019)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aattctgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga ggctctaagg
       61 gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt
      121 aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt
      181 tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag
      241 cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca
      301 tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt
      361 actatcaaca ggttgaactg ctgatcaaca gatcctctac gcggccgcgg taccataact
      421 tcgtatagca tacattatac gaagttatct ggtttaaacg tacccgtagt ggctatggca
      481 gggcttgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tgggggttgg
      541 ggtggggaaa aggaagaaac gcgggcgtat tggtcccaat ggggtctcgg tggggtatcg
      601 acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacacccgtg
      661 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
      721 ccgtgtttca gttagcctcc cccatctccc ggtaccgcat gctatgcatc agctgctagc
      781 accatggctc gagatcccgg gtgatcaagt cttcgtcgag tgattgtaaa taaaatgtaa
      841 tttacagtat agtattttaa ttaatataca aatgatttga taataattct tatttaacta
      901 taatatattg tgttgggttg aattaaaggt ccgtatacta gtatcgattc gcgacctact
      961 ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag
     1021 tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt
     1081 cataccgtcc caccatcggg cgcggatccc ggtccgaagc gcgcggaatt caaaggccta
     1141 cgtcgacgag ctcactagtc gcggccgctt tcgaatctag agcctgcagt ctcgacaagc
     1201 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     1261 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     1321 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1441 tatcttatca tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa
     1501 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccccta
     1561 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatct
     1621 gaacaggagg gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat
     1681 acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat aaatacctgt
     1741 gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc
     1801 ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca
     1861 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc
     1921 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg
     1981 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac
     2041 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     2101 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc
     2161 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     2221 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     2281 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     2341 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     2401 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata
     2461 ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg
     2521 tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca
     2581 gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc
     2641 ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc
     2701 gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta
     2761 ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag
     2821 gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc ccagctgaca
     2881 ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag
     2941 cagcccttgc gccctgagtg cttgcggcag cgtgaagct
//
