LOCUS       sequence-208931-        2857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for the insect cell expression system, donor for plasmid 
            fusions, two promoters, Multiplication Module M, LoxP site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, 
            Berger I
  TITLE     Protein complex expression by using multigene baculoviral vectors.
  JOURNAL   Nat Methods. 2006 Dec;3(12):1021-32. doi: 10.1038/nmeth983.
  PUBMED    17117155
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Methods"; date: "2006-12"; volume: "3"; issue: "12"; pages: 
            "1021-32"
COMMENT     Sequence Label: pSPL
FEATURES             Location/Qualifiers
     source          1..2857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..362
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(415..448)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    638..686
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     711..730
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(822..931)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        976..1067
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     992..1009
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    1326..1460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1363..1382)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1417..1436
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(1610..2401)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aattctgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga ggctctaagg
       61 gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaagcttt
      121 aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt
      181 tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag
      241 cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca
      301 tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt
      361 actatcaaca ggttgaactg ctgatcaaca gatcctctac gcggccgcgg taccataact
      421 tcgtatagca tacattatac gaagttatct ggtttaaacg tacccgtagt ggctatggca
      481 gggcttgccg ccccgacgtt ggctgcgagc cctgggcctt cacccgaact tgggggttgg
      541 ggtggggaaa aggaagaaac gcgggcgtat tggtcccaat ggggtctcgg tggggtatcg
      601 acagagtgcc agccctggga ccgaaccccg cgtttatgaa caaacgaccc aacacccgtg
      661 cgttttattc tgtcttttta ttgccgtcat agcgcgggtt ccttccggta ttgtctcctt
      721 ccgtgtttca gttagcctcc cccatctccc ggtaccgcat gctatgcatc agctgctagc
      781 accatggctc gagatcccgg gtgatcaagt cttcgtcgag tgattgtaaa taaaatgtaa
      841 tttacagtat agtattttaa ttaatataca aatgatttga taataattct tatttaacta
      901 taatatattg tgttgggttg aattaaaggt ccgtatacta gtatcgattc gcgacctact
      961 ccggaatatt aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag
     1021 tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt
     1081 cataccgtcc caccatcggg cgcggatccc ggtccgaagc gcgcggaatt caaaggccta
     1141 cgtcgacgag ctcactagtc gcggccgctt tcgaatctag agcctgcagt ctcgacaagc
     1201 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     1261 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     1321 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1381 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1441 tatcttatca tgtctggatc tgatcactgc ttgagcctag aagatccggc tgctaacaaa
     1501 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactatc ataaccccta
     1561 ggagtccaag cgagctcgat atccgtcggc ttgaacgaat tgttagacat tatttgccga
     1621 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg
     1681 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct
     1741 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
     1801 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc
     1861 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
     1921 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
     1981 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
     2041 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat
     2101 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
     2161 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
     2221 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
     2281 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
     2341 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
     2401 tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
     2461 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     2521 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     2581 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctactta ccggtttatt
     2641 gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt ttgctcaggc
     2701 tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc agctgacatt
     2761 catccggggt cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca
     2821 gcccttgcgc cctgagtgct tgcggcagcg tgaagct
//
