LOCUS       sequence-208967-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of HUWE1 HECT domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Michel MA, Swatek KN, Hospenthal MK, Komander D
  TITLE     Ubiquitin Linkage-Specific Affimers Reveal Insights into K6-Linked 
            Ubiquitin Signaling.
  JOURNAL   Mol Cell. 2017 Oct 5;68(1):233-246.e5. doi: 
            10.1016/j.molcel.2017.08.020. Epub 2017 Sep 21.
  PUBMED    28943312
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.08.020"; journalName: "Mol Cell"; date: 
            "2017-10-5- 5"; volume: "68"; issue: "1"; pages: "233-246.e5"
COMMENT     Sequence Label: Human HUWE1 (3993-4374)
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..87
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca tcacagcagc
       61 ggtctggaag ttctgtttca gggcccggac tttgatgtca agcgcaaata tttccgccaa
      121 gagctggagc gtttagatga ggggctccgg aaagaagaca tggctgtgca tgtccgtcgt
      181 gaccatgtgt ttgaagactc ctatcgtgag ctgcatcgca aatcccccga agaaatgaag
      241 aatcgattgt atatagtatt tgaaggagaa gaagggcagg atgctggcgg gctcctgcgg
      301 gagtggtata tgatcatctc tcgagagatg tttaacccta tgtatgcctt gttccgtacc
      361 tcacctggtg atcgagtcac ctacaccatc aatccatctt cccactgcaa ccccaaccac
      421 ctcagctact tcaagtttgt cggacgcatt gtggccaaag ctgtatatga caaccgtctt
      481 ctggagtgct actttactcg atccttttac aaacacatct tgggcaagtc agtcagatat
      541 acagatatgg agagtgaaga ttaccacttc taccaaggtc tggtttatct gctggaaaat
      601 gatgtctcca cactaggcta tgacctcacc ttcagcactg aggtccaaga gtttggagtt
      661 tgtgaagttc gtgacctcaa acccaatggg gccaacatct tggtaacaga ggagaataag
      721 aaggagtatg tacacctggt atgccagatg agaatgacag gagccatccg caagcagttg
      781 gcggctttct tagaaggctt ctatgagatc attccaaagc gcctcatttc catcttcact
      841 gagcaggagt tagagctgct tatatcagga ctgcccacca ttgacatcga tgatctgaaa
      901 tccaacactg aataccacaa gtaccagtcc aactctattc agatccagtg gttctggaga
      961 gcattgcgtt ctttcgatca agctgaccgt gccaagttcc tccagtttgt cacgggtact
     1021 tccaaggtac ccctgcaagg ctt
//
