LOCUS       sequence-208970-         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human Ubiquitin (1-76; T66V, L67N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Gladkova C, Schubert AF, Wagstaff JL, Pruneda JN, Freund SM, 
            Komander D
  TITLE     An invisible ubiquitin conformation is required for efficient 
            phosphorylation by PINK1.
  JOURNAL   EMBO J. 2017 Dec 15;36(24):3555-3572. doi: 10.15252/embj.201797876. 
            Epub 2017 Nov 13.
  PUBMED    29133469
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201797876"; journalName: "EMBO J"; date: "2017-12-15-
            15"; volume: "36"; issue: "24"; pages: "3555-3572"
COMMENT     Sequence Label: Ubiquitin (1-76; T66V, L67N)
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(299..317)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      313..360
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(405..423)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        491..595
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tggtaagacc atcactctcg aagtggagcc gagtgacacc attgagaatg tcaaggcaaa
       61 gatccaagac aaggaaggca tccctcctga ccagcagagg ttgatctttg ctgggaaaca
      121 gctggaagat ggacgcaccc tgtctgacta caacatccag aaagagtccg tcaatcacct
      181 ggtcctccgt ctcagaggtg ggtgataaga attctgcaga tatccatcac actggcggcc
      241 gctcgagcag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      301 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      361 ctgaaaggag gaactatatc cggataattc ttgaagacga aagggcctcg tgatacgcct
      421 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
      481 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      541 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
      601 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
      661 tgctc
//
