LOCUS       sequence-208972-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human Ubiquitin (1-76; L71Y).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Gladkova C, Schubert AF, Wagstaff JL, Pruneda JN, Freund SM, 
            Komander D
  TITLE     An invisible ubiquitin conformation is required for efficient 
            phosphorylation by PINK1.
  JOURNAL   EMBO J. 2017 Dec 15;36(24):3555-3572. doi: 10.15252/embj.201797876. 
            Epub 2017 Nov 13.
  PUBMED    29133469
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201797876"; journalName: "EMBO J"; date: "2017-12-15-
            15"; volume: "36"; issue: "24"; pages: "3555-3572"
COMMENT     Sequence Label: Ubiquitin (1-76; L71Y)
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(73..300)
                     /codon_start=1
                     /gene="Homo sapiens UBC"
                     /product="human ubiquitin C"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVYRLRGG"
     RBS             308..330
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(361..380)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(362..380)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcttat cacccacctc tgagacgata gaccaggtgc agggtggact ctttctggat
      121 gttgtagtca gacagggtgc gtccatcttc cagctgtttc ccagcaaaga tcaacctctg
      181 ctggtcagga gggatgcctt ccttgtcttg gatctttgcc ttgacattct caatggtgtc
      241 actcggctcc acttcgagag tgatggtctt accagtcagg gtcttcacga agatctgcat
      301 atgtatatct ccttcttaaa gttaaacaaa attatttcta gagggaaacc gttgtggtct
      361 ccctatagtg agtcgtatta atttcgcggg atcgagatct cgggcagcgt tgggtcctgg
      421 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
      481 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
      541 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
      601 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
      661 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga
      721 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc
//
