LOCUS       sequence-208973-         514 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Ubiquitin (1-76; F4A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Gladkova C, Schubert AF, Wagstaff JL, Pruneda JN, Freund SM, 
            Komander D
  TITLE     An invisible ubiquitin conformation is required for efficient 
            phosphorylation by PINK1.
  JOURNAL   EMBO J. 2017 Dec 15;36(24):3555-3572. doi: 10.15252/embj.201797876. 
            Epub 2017 Nov 13.
  PUBMED    29133469
REFERENCE   2  (bases 1 to 514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.15252/embj.201797876"; journalName: "EMBO J"; date: "2017-12-15-
            15"; volume: "36"; issue: "24"; pages: "3555-3572"
COMMENT     Sequence Label: Ubiquitin (1-76; F4A)
FEATURES             Location/Qualifiers
     source          1..514
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(96..323)
                     /codon_start=1
                     /gene="Homo sapiens UBC"
                     /product="human ubiquitin C"
                     /label=ubiquitin
                     /translation="MQIAVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     RBS             331..353
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(384..403)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(385..403)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctcgagcgg ccgccagtgt gatggatatc tgcagaattc cagcacactg gcggccgtta
       61 ctagtggatc cgagctcggt accaagcttt tatcacccac ctctgagacg gaggaccagg
      121 tgcagggtgg actctttctg gatgttgtag tcagacaggg tgcgtccatc ttccagctgt
      181 ttcccagcaa agatcaacct ctgctggtca ggagggatgc cttccttgtc ttggatcttt
      241 gccttgacat tctcaatggt gtcactcggc tccacttcga gagtgatggt cttaccagtc
      301 agggtcttca cggcgatctg catatgtata tctccttctt aaagttaaac aaaattattt
      361 ctagagggaa accgttgtgg tctccctata gtgagtcgta ttaatttcgc gggatcgaga
      421 tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
      481 cccggctagg ctggcggggt tgccttactg gtta
//
