LOCUS       sequence-208975-       12171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of shRNA resistant Psd95-EGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Schluter OM, Xu W, Malenka RC
  TITLE     Alternative N-terminal domains of PSD-95 and SAP97 govern 
            activity-dependent regulation of synaptic AMPA receptor function.
  JOURNAL   Neuron. 2006 Jul 6;51(1):99-111.
  PUBMED    16815335
REFERENCE   2  (bases 1 to 12171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2006-07-6"; volume: "51(1)"; pages: "99-111"
COMMENT     Sequence Label: FUp95GW (B7)
FEATURES             Location/Qualifiers
     source          1..12171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2621..3832
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3669..3688
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6096..6105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6102..6104,6105..6107,6108..6821)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6102..6104,6105..6107,6108..6818)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6147..6168)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6408..6427)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6755..6776
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6862..7450
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6915..6935)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7333..7344)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7453..7469)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7454..7470)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7975..8155
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8181..8198)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8187..8411
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8457..8885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8544..8563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8754..8775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8894..8914)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8899..9228
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9079..9214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9141..9160
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9276..9323
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9342..9716
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9846..9967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9883..9902)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9937..9956
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10016..10032)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10016..10032)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10029..10051)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10040..10056
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10064..10070,10071..10088,10089..10094))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10109..10130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10247..10264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10418..11006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10498..10517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11177..11968,11969..12037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11800..11819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12038..12142)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttaattaa cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg
     2641 gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc
     2701 agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac
     2761 tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct
     2821 agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc
     2881 gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg
     2941 ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat
     3001 cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg
     3061 ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc
     3121 cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc
     3181 ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg
     3241 gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct
     3301 tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga
     3361 ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca
     3421 gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt
     3481 ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg
     3541 caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg
     3601 gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct
     3661 taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga
     3721 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
     3781 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
     3841 ggctgcaggt cgactctaga ggatccccgg gtaccggaat tcgccctttc tagaccacca
     3901 tggactgtct ctgtatagtg acaaccaaga aataccgcta ccaagatgaa gacacgcccc
     3961 ctctggaaca cagcccggcc cacctcccca accaggccaa ttctccccct gtgattgtca
     4021 acacggacac cctagaagcc ccaggatatg agttgcaggt gaatggaaca gagggggaga
     4081 tggagtatga ggagatcaca ttggaaaggg gtaactcagg tctgggcttc agcatcgcag
     4141 gtggcactga caacccgcac atcggtgacg acccgtccat ttttatcacc aagatcattc
     4201 ctggtggggc tgcagcccag gatggccgcc tcagggtcaa tgacagcatc ctgtttgtaa
     4261 atgaagtgga cgtacgagag gtgacccatt cagctgcggt ggaggccctc aaagaggcag
     4321 gttccatcgt tcgcctctat gtcatgcgcc ggaaaccccc agccgaaaag gtcatggaga
     4381 tcaaactcat caaagggcct aaaggacttg gcttcagcat tgcggggggc gttgggaacc
     4441 agcacatccc tggagataac agcatctatg taacgaagat catcgaagga ggtgctgccc
     4501 acaaggatgg caggttgcag attggagaca agatcctggc ggtcaacagt gtggggctgg
     4561 aggacgtcat gcacgaggat gccgtggcag ccctgaagaa cacatatgac gttgtgtacc
     4621 taaaggtggc caagcccagc aatgcctacc tgagtgacag ctatgctccc ccagacatca
     4681 caacctcgta ttctcagcac ctggacaatg agatcagtca tagcagctac ttgggcactg
     4741 actaccccac agccatgacc cccacttccc ctcggcgcta ctcccctgtg gccaaggacc
     4801 tgctggggga ggaagacatt ccccgggaac caaggcggat cgtgatccat cggggctcca
     4861 ccggcctggg cttcaacatc gtgggcggcg aggatggtga aggcatcttc atctccttca
     4921 tccttgctgg gggtccagcc gacctcagtg gggagctacg gaagggggac cagatcctgt
     4981 cggtcaatgg tgttgacctc cgcaatgcca gtcacgaaca ggctgccatt gccctgaaga
     5041 atgcgggtca gacggtcacg atcatcgctc agtataaacc agaagagtat agtcgattcg
     5101 aggccaagat ccatgatctt cgggaacagc tcatgaatag tagcctaggc tcagggactg
     5161 catccttgcg aagcaacccc aagaggggct tctacattag ggccctgttt gattacgaca
     5221 agaccaagga ctgcggtttc ttgagccagg ccctgagctt ccgcttcggg gatgtgcttc
     5281 atgtcattga cgctggtgac gaagagtggt ggcaagcacg gcgggtccac tccgacagtg
     5341 agaccgacga cattggcttc attcccagca aacggcgggt cgagcgacga gagtggtcaa
     5401 ggttaaaggc caaggactgg ggctccagct ctggatcaca gggtcgagaa gactcggttc
     5461 tgagctatga gacggtgacc cagatggaag tgcactatgc tcgtcccatc atcatccttg
     5521 gacccaccaa agaccgtgcc aacgatgatc ttctctccga gttccccgac aagtttggat
     5581 cctgtgtccc tcatacgaca cgtcctaagc gggaatatga gatagacggc cgggattacc
     5641 actttgtctc ctcccgggag aaaatggaga aggacatcca ggcacacaag ttcattgagg
     5701 ctggccagta caacagccac ctctatggga ccagcgtcca gtctgtgcga gaggtagcag
     5761 agcaggggaa gcactgcatc ctcgatgtct cggccaatgc cgtgcggcgg ctgcaggcgg
     5821 cccacctgca ccccatcgcc atcttcatcc gtccccgctc cctggagaat gtgctagaga
     5881 tcaataagcg gatcacagag gagcaagccc ggaaagcctt cgacagagcc acgaagctgg
     5941 agcaggagtt cacagagtgc ttctcagcca tcgtagaggg cgacagcttt gaagagatct
     6001 atcacaaagt gaaacgtgtc attgaagacc tctcaggccc ctacatctgg gtcccagccc
     6061 gagagagact cggtaccgcg ggcccgggat ccatcgccac catggtgagc aagggcgagg
     6121 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     6181 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     6241 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     6301 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     6361 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     6421 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     6481 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     6541 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     6601 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     6661 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     6721 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     6781 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gactctagaa
     6841 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     6901 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6961 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7021 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7081 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7141 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7201 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7261 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7321 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7381 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7441 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     7501 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     7561 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     7621 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7681 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     7741 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     7801 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     7861 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     7921 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     7981 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8041 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8101 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     8161 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     8221 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     8281 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8341 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     8401 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     8461 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8521 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8581 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8641 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8701 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8761 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8821 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8881 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     8941 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9001 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9061 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9121 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     9181 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     9241 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     9301 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     9361 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     9421 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     9481 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     9541 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     9601 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     9661 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     9721 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     9781 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     9841 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9901 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9961 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
    10021 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    10081 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    10141 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    10201 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    10261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    10861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    11101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    11161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    11221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    11281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    12001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    12061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    12121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac g
//
