LOCUS       sequence-20899-e         554 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GAL-EYFP-ccdB
FEATURES             Location/Qualifiers
     source          1..554
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     212..233
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    322..439
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(463..483)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(463..481)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(515..537)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    526..542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
       61 gcgaagcact gcaggccgta gccgaaggtg gtcacgaggg tgggccaggg cacgggcagc
      121 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
      181 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      241 accaccccgg tgaacagctc ctcgcccttg ctcaccattt tgtcccgggg gatccactag
      301 tattgtagaa tctttattgt tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa
      361 cgcgaccggt gaagacgagg acgcacggag gagagtcttc cttcggaggg ctgtcacccg
      421 ctcggcggct tctaatccgt actagagctc cagcttttgt tccctttagt gagggttaat
      481 tgcgcgcttg gcgtaatcat ggtcatanct gtttcctgtg tgaaattgtt atccgctcac
      541 aattccacac aaca
//
