LOCUS       sequence-208992-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  URT-type vector for Val-mI-kappa-B-alpha expression in Mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Piatkov KI, Oh JH, Liu Y, Varshavsky A
  TITLE     Calpain-generated natural protein fragments as short-lived 
            substrates of the N-end rule pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):E817-26. doi: 
            10.1073/pnas.1401639111. Epub 2014 Feb 18.
  PUBMED    24550490
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1401639111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-03-4- 4"; volume: "111"; issue: "9"; pages: "E817-26"
COMMENT     Sequence Label: pJO428
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttatggac tacaaggacg acgatgacaa gggtgcacga
      121 caggtttccc gacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      181 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
      241 tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct
      301 gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc
      361 tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatgga
      421 tccatggttc gaccattgaa ctgcatcgtc gccgtgtccc aaaatatggg gattggcaag
      481 aacggagacc taccctggcc tccgctcagg aacgagttca agtacttcca aagaatgacc
      541 acaacctctt cagtggaagg taaacagaat ctggtgatta tgggtaggaa aacctggttc
      601 tccattcctg agaagaatcg acctttaaag gacagaatta atatagttct cagtagagaa
      661 ctcaaagaac caccacgagg agctcatttt cttgccaaaa gtttggatga tgccttaaga
      721 cttattgaac aaccggaatt ggcaagtaaa gtagacat
//
