LOCUS       sequence-209-exp       10970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10970)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 10970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3809
FEATURES             Location/Qualifiers
     source          1..10970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3600..3628)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4376..4396
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        8715..8819
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8820..8888,8889..9680)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9038..9057)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9851..10439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10340..10359
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10593..10610
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10727..10748
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10763..10768,10769..10786,10787..10793)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10801..10817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10806..10828
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10825..10841
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10825..10841
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttctgg agattgctcc agcagctctt aacagtgtct acgaccgtct tcgtgcaatt
       61 ctcatggaaa ctgaaagatc ggaaaatgca ctggatcgac gtattcagta tatgattgag
      121 actgcaatgc agattcgaaa ggacaaattt gcggtaaggt agaatatata aatagtttat
      181 tagaaaaaaa taaattagaa taatttaaat tcctactagc caatcaggcg acctttttgc
      241 gcatagttct attattgaaa aatttggaga atttctcata ttctcgctcg gaaatctgga
      301 attcgacgag atcttctggc ttctgtgcag ctgcatcgct ttgtgctccc tttctcgctt
      361 gtcttctgtg tacaccaaga accttgttga gttcatcaac tgaatctgtg actggcttgt
      421 tgctcactgg atgcactaga cgactgattc tcgagaaatc agattgagtt gcgattaggg
      481 tgacctagaa attgggaata atacgaactt ttgaaaatat tcaggaggat taaaaaaatt
      541 attctcgaca atcctacaaa tttacttatt gcaccatgtt gctccaacat ttttcattaa
      601 aagttaatga aaaaatgtag aaaatcggaa attggcaatt ttcagaccat ttttaagcat
      661 tttcaaaaaa aaattgcagc tgaaataaat gtcattttca gataaatcga gcgattttct
      721 gttgtctgac actagttttt agttttaaaa aatgttggaa gaacatggtg caataggtaa
      781 tttcatagaa tttccatgtg ttttttttca attaaccaat tatccaaatc ttccaaactc
      841 acattttgcg gagctgggct atcaagaatc tgctgcagtt ttataagacg agcatctctg
      901 atatcactga aaattaattt ttaatcaaaa cttgaatatc aactaaaccc acttattaac
      961 tttctcgatc ttctgtcgtt cggtacgatg acggtgaaga agccaattgt agtagttgat
     1021 ttggttcaag tcctttcggt gttgtacgtc agtgtcctgc aatgctattt agttataact
     1081 taggcctaag attcaattta atgaagtgat taaatttgtt ctctgaacct cttaagatga
     1141 tcttttggat tagaaacata taagacaggt ttacctatct attaaaaaac agatcaaaat
     1201 agatacgacc aaatcggata atccatgcct acctggcatc taggaacgtg ttcttagaag
     1261 atttcttacg taatcgtatg aagaaataac aatttgatcg ttggccagca aaaatagggt
     1321 tttaagtggg atagtgtttt tattagctaa ccggaaaatt ttatagtttt tttttgcaag
     1381 aaaccactga aaacccccta attgtataca ttttttggag cagcttctgg tctttttgag
     1441 caataaaatt cgataaaaca gaatttaagt gtaaattgtt cacatttagt ttctatttta
     1501 tcaaattttg ttgctcaaaa acattcgaag ctgctctaaa aaaatgcatt aaaaaagggg
     1561 ttttcagtgg tttttcacat taaaaaagct aattttaact aaaaatccat catatttcca
     1621 actttgtcac aacaataaaa tgctggtcaa aatgtgttcg aaaaaatgtt ttttttttta
     1681 atttttataa tttaaaaata gttttctttc gctgggacac atacattttt gggcgtaaat
     1741 tttcagttca aatttccatt tttacaacca taatcataaa gctacgtctg atctctctcg
     1801 cacttacctg cgcctgattc gaaagaacaa ccgtagccaa aagaacaaga agaacaagca
     1861 cgtagttgtg gtagtggacg ttcatcacgc aatactgacc aatggtcgtg gggtctcact
     1921 ttccgtacta ttgagagagg ggagactgaa gatggcaatt gaggacagtg tcttcgacgc
     1981 acgcatgcat ccataagcat aatccaggag ggatggagag aaaaatcttg tttctaagcc
     2041 cctccctttg taatacatac acatatctaa taccgaagaa tggctaattg aatggacgtc
     2101 agctgttgct gtagttgcca aggcatcatc gatgaaataa ctgaaagaaa gaattaaata
     2161 attattgcag gcgtatccgg cggtcattga agacttggac ttgattgagg aggaggatca
     2221 gatcatccat acacttaatt tggaggatgc ggttgatccg gaaaatgggc ttagtaagtg
     2281 actgaccaca cgcggggggc attaatttaa taaattgaat tccatttcag atgtgttcaa
     2341 actagatcca gaattcgaaa agaacgagga ggtttatgag gagatccgta aggaaatcat
     2401 tggaaacgcc gatatttcgg atgaggatgg tggcgacgag ttggatgatg aagaagaggg
     2461 tagtgatgtg gaagaggctc cgaagaagac tacagagatt attgataata ctgatcagaa
     2521 ttgactgctt tcagaaggta ttcattttga gttttgggcc ggcaaatctg taagttgccg
     2581 gttgccgaaa atttgctgaa tttgccggaa aaaaaaattc cggaatttat ttaaaaactt
     2641 tttgtaaaaa ttaaattaaa tttgcaactt ttcagagaag tctacctgac aatgcaatca
     2701 tctttggact accaagaagc tgctcacaaa ttgctgaaaa tgaagattcc agacagcatg
     2761 caggtcagcg atgttgcaaa gaaaaatttt cgaccaaaaa aaccaaccaa tcataaaatt
     2821 taaaaaaaaa ctccgttttt ttcttttttt ttatacgaga aaaaccaaaa aaatgtattt
     2881 ttgccaaatt ctaaaatact atccccgaaa ttttcaatat tttctctttc agaacgaact
     2941 ctgcgcgatg cttgtcgatt gttgtgctca acagcgtacc tacgagcgat tctacggaat
     3001 gctgagaaca cgttaaacac gaagatcata tttattttga gacccggatg ctctgaaaat
     3061 gtctgacata gatttaaaaa agcatatata tatttttcat tttcaacgtg aaagttttgt
     3121 gcaactttat agaatctcct attggcacat tgttttttat ttaactgagg cagtttttga
     3181 acaccttttt gaaactttga atctctttga agtatactgt cgaaaagact gacttgagcg
     3241 ttcgaaatgc cagaagaaaa ctatatttga atctcgcgct aaattgagaa atgcaaccgc
     3301 gctccactgg acaattggaa aaaaaattta ttcggaggcg acaacggtat tttcgaaatt
     3361 gattttctgt gtattttctc attttttata aattcttctt tgatttatcg ttcgtttgtg
     3421 agaaatttaa ttgtattcaa acttttttat agtaagatac cggtggtacc gctagccgta
     3481 cgaacccggg ttctagagga tcctatcgat tcgcggccgc tgtacacccg ggtgccctat
     3541 agtgagtcgt attggtaccg gtagaaaaaa tgagtaaagg agaagaactt ttcactggag
     3601 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     3661 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
     3721 gaaaactacc tgttccatgg gtaagtttaa acatatatat actaactaac cctgattatt
     3781 taaattttca gccaacactt gtcactactt tctgttatgg tgttcaatgc ttctcgagat
     3841 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
     3901 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtaag tttaaacagt
     3961 tcggtactaa ctaaccatac atatttaaat tttcaggtgc tgaagtcaag tttgaaggtg
     4021 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     4081 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     4141 aaaagaatgg aatcaaagtt gtaagtttaa acatgatttt actaactaac taatctgatt
     4201 taaattttca gaacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     4261 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     4321 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     4381 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggacgaacta tacaaaagcc
     4441 cacaagcttt ggaattcgct agccggccat acaagtaatc cgggttctag aagcgctgct
     4501 aagggggccc tcgtcgagtc ggtcacaatc acctgaaact ccaaaggcag ccagtgagga
     4561 acgtgaagaa gaagaaaaag agtcatctga acaggtttga ttttctttct ggtcaaaaag
     4621 atgaaattat tgattttcag ccagatactc ccaaaactag cagcgagaag tctgcaagtc
     4681 gttcacagtc gcccagagaa tcgcgggaag tgagccaaga ggtatgtttt tcaaaaatca
     4741 ataactgatc ataattttta ttgtttggtg aatttaagaa aataatattc gaaaattcct
     4801 ctgaattatc aagattgcag tattaatttc gagaaaaatt gagatattca tagagctatt
     4861 gtaaattttc ttgatttcag actgaaactt cggaaaatca agagaaaatc aaagaaaagg
     4921 atgacgggga tgatcagcct ggcacaccga acagctatag aagccgggaa acttcaccag
     4981 ctccaaaaag gtccaaggag accaggtttg tcaaaagctt cctgcgatta attctcattt
     5041 caatttttca gagaatcaga gtctcctgaa aaatccccgg ttcgttcaag atctcccaga
     5101 aggtcttcag cacgttcccc gtcacgatct cctagacggc gccgagaaag aagctcagaa
     5161 agaaagcaat ccgaagagcc agcaccgcta ccagagaaaa agaagaaaga gccgctggat
     5221 attctacgaa caagaaccgg aggagcatat attccacccg ccaaacttcg acttatgcaa
     5281 caacagatta gtgataagca aagtgaacag tatcagagaa tgaattggga aagaatgaag
     5341 aaaaagattc acggattggt taacagagtc aacgcgaaga atcttgttca aattgtcaga
     5401 gaacttcttc aagagaatgt gattcgttca aagtgagtga gaaaatcgaa ggaaaaggaa
     5461 agaattaatt taatttttca ggggacttct ctgccgtgac attattcaag ctcaggcttt
     5521 ctcaccagga ttctctaacg tctatgcagc tttggcggca gttatcaact cgaaattccc
     5581 tcatgtcggt gaacttcttc tccgtcgtct gattgtacag ttcaaaagaa gtttccgtag
     5641 aaatgacaga ggcgtcacgg tgaacgtgat caaattcatc gcacatttga ttaatcaaca
     5701 agttgctcac gaagttcttg cgctggaaat catgattctg atgcttgaag aaccaactga
     5761 tgattcagtt gaagtcgcca ttgcgttcct gaaagagtgt ggagcaaagc ttctggagat
     5821 tgctccagca gctcttaaca gtgtctacga ccgtcttcgt gcaattctca tggaaactga
     5881 aagatcggaa aatgcactgg atcgacgtat tcagtatatg attgagactg caatgcagat
     5941 tcgaaaggac aaatttgcgg cgtatccggc ggtcattgaa gacttggact tgattgagga
     6001 ggaggatcag atcatccata cacttaattt ggaggatgcg gttgatccgg aaaatgggct
     6061 taatgtgttc aaactagatc cagaattcga aaagaacgag gaggtttatg aggagatccg
     6121 taaggaaatc attggaaacg ccgatatttc ggatgaggat ggtggcgacg agttggatga
     6181 tgaagaagag ggtagtgatg tggaagaggc tccgaagaag actacagaga ttattgataa
     6241 tactgatcag aattgactgc tttcagaagg tattcatttt gagttttggg ccggcaaatc
     6301 tgtaagttgc cggttgccga aaatttgctg aatttgccgg aaaaaaaaat tccggaattt
     6361 atttaaaaac tttttgtaaa aattaaatta aatttgcaac ttttcagaga agtctacctg
     6421 acaatgcaat catctttgga ctaccaagaa gctgctcaca aattgctgaa aatgaagatt
     6481 ccagacagca tgcaggtcag cgatgttgca aagaaaaatt ttcgaccaaa aaaaccaacc
     6541 aatcataaaa tttaaaaaaa aactccgttt ttttcttttt ttttatacga gaaaaaccaa
     6601 aaaaatgtat ttttgccaaa ttctaaaata ctatccccga aattttcaat attttctctt
     6661 tcagaacgaa ctctgcgcga tgcttgtcga ttgttgtgct caacagcgta cctacgagcg
     6721 attctacgga atgctcatcg aacgtttctg ccgacttcgc ctcgaatacc agcaatactt
     6781 tgaaaagctc tgccaggaca cgtattccac gattcaccga attgacatca caaaactgcg
     6841 gaatttggct cgccttattg ctcatttgct ctcgacggat gctattgact ggaagatttt
     6901 ggccgatatg aaaatgaccg aagaggacac aacttcttct ggcagaatct atattaaata
     6961 tatatttaat gaacttgtgg aggcgatggg aatggttaaa cttcattcga gagttactga
     7021 tccgtgagtt tcctagagag agttgttttc gtattcaatt ttccctattt tcagaacttt
     7081 ggctcattgc tttgttggat tattcccacg aactaatccg aacagcgcac gattttcgat
     7141 caacttcttc acaatgattg gattgggtgg tttgacgttg gaacttcgtg aatggctggc
     7201 aaagggtctc aagaagaaga agggaatgct ggatcagttg aaggccgaat caagctcaga
     7261 ttcatcgtcg tcttcggatt cgtcagactc gtctgattct tcggattctg acgattcatc
     7321 cgactcgtct tcagattcct catcttcttc agaatcagag ccagaaccac cgaagaaaaa
     7381 gaagaagaag aacagtgaag agagttccaa aaagaaggaa aaagagaata ttggtcgacg
     7441 ggatcgtgga gacaagagag ctgaacgtca tcgtgatcaa agtgtggaga acaaggacaa
     7501 ggatcgtcga cgtcgccagg attctgacga aaatcgtcgg ccagaacgag gagatgaccg
     7561 caaggatcgg agtaaagatc gtcgtcgtca agactcggat gatgaggatc ggaaaggtcg
     7621 tgaacgtcgg gaagattcag gggaaagacg tcgcggagat cgggatcgac gtgatcgaaa
     7681 caaggatcag gaggatcacc gtgaagatcg ccgtgaccga agcaaggatc gtgaggatcg
     7741 acgtgatcgc cgtcgtcatg actctgatga tgatcgtaaa actcgtcggg atagaagtga
     7801 agagcgagga ggacgtcgtc gtgaagtgga atcggatgat cgacgccgac gtcgttgaat
     7861 tttcaaattt taaatactga atatttgttt tttttcctat tatttattta ttctctttgt
     7921 gttttttttc ttgctttcta aaaaattaat tcaatccaaa tctaaacatt tttttttctc
     7981 tttccgtctc ccaattcgta ttccgctcct ctcatctgaa cacaatgtgc aagtttattt
     8041 atcttctcgc tttcatttca ttaggacgtg gggggaattg gtggaagggg gaaacacaca
     8101 aaaggatgat ggaaatgaaa taaggacaca caatatgcaa caacattcaa ttcagaaata
     8161 tggaggaagg tttaaaagaa aacataaaaa tatatagagg aggaaggaaa actagtaaaa
     8221 aataagcaaa gaaattaggc gaacgatgag aattgtcctc gcttggcaaa tgcgaatccg
     8281 tatggagagg cacgtttggc gaaggcaaat gttcggtatg gagatctgta aaaattttta
     8341 agttgaaatt tggtgttgct cttttacaaa attttccgat tttcgcttga aattacggtg
     8401 ccaggtctcg acacgtcttc caatttttca aattcaaaag agcctttaat gggctgtagt
     8461 tgctaatttc tcgtttttga aaatttttct tccgtttaat cgaaatttga tgtattttat
     8521 ttatgatttt caataaattt caaagaaact ggtgaaaact cggaaaattg tgaactacag
     8581 taatccaatc cttaaaggcg cacacctttt aaatgtccgc cccaatacga tattttttta
     8641 agattcgcta gagcggccca aaaggccttt tttttttggc gccgacgtca ggtggcactt
     8701 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8761 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8821 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8881 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8941 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9001 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9061 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9121 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9181 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9241 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9301 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9361 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9421 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9481 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9541 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9601 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9661 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9721 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9781 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9841 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9901 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9961 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    10021 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10081 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10141 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10201 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
    10261 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10321 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10381 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10441 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10501 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10561 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10621 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    10681 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    10741 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    10801 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctgt
    10861 aagtttaaac atgatcttac taactaacta ttctcattta aattttcaga gcttaaaaat
    10921 ggctgaaatc actcacaacg atggatacgc taacaacttg gaaatgaaat
//
