LOCUS       sequence-209003-        5823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Mitochondrial Matrix, Excitation: 505 / 569, Emission:
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5823)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-PDHA1-N-10
FEATURES             Location/Qualifiers
     source          1..5823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        54..357
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        358..561
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     512..532
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1793..1802
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1799..2482
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     polyA_signal    2604..2725
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2641..2660)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2695..2714
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2732..3187)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2869..2890)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3081..3100
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3214..3318
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3320..3677
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3320..3511
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3343..3363)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3528..3663
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3712..4506
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3712..4506
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3766..3785)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4376..4395
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4694..4713)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4738..4785
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5114..5702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5603..5622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
       61 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      121 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      181 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      241 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      301 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      361 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
      421 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      481 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
      541 gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagca ccgccaccat
      601 gaggaagatg ctcgccgccg tctcccgcgt gctgtctggc gcttctcaga agccggcaag
      661 cagagtgctg gtagcatccc gtaattttgc aaatgatgct acatttgaaa ttaagaaatg
      721 tgaccttcac cggctggaag aaggccctcc tgtcacaaca gtgctcacca gggaggatgg
      781 gctcaaatac tacaggatga tgcagactgt acgccgaatg gagttgaaag cagatcagct
      841 gtataaacag aaaattattc gtggtttctg tcacttgtgt gatggtcagg aagcttgctg
      901 tgtgggcctg gaggccggca tcaaccccac agaccatctc atcacagcct accgggctca
      961 cggctttact ttcacccggg gcctttccgt ccgagaaatt ctcgcagagc ttacaggacg
     1021 aaaaggaggt tgtgctaaag ggaaaggagg atcgatgcac atgtatgcca agaacttcta
     1081 cgggggcaat ggcatcgtgg gagcgcaggt gcccctgggc gctgggattg ctctagcctg
     1141 taagtataat ggaaaagatg aggtctgcct gactttatat ggcgatggtg ctgctaacca
     1201 gggccagata ttcgaagctt acaacatggc agctttgtgg aaattacctt gtattttcat
     1261 ctgtgagaat aatcgctatg gaatgggaac gtctgttgag agagcggcag ccagcactga
     1321 ttactacaag agaggcgatt tcattcctgg gctgagagtg gatggaatgg atatcctgtg
     1381 cgtccgagag gcaacaaggt ttgctgctgc ctattgtaga tctgggaagg ggcccatcct
     1441 gatggagctg cagacttacc gttaccacgg acacagtatg agtgaccctg gagtcagtta
     1501 ccgtacacga gaagaaattc aggaagtaag aagtaagagt gaccctatta tgcttctcaa
     1561 ggacaggatg gtgaacagca atcttgccag tgtggaagaa ctaaaggaaa ttgatgtgga
     1621 agtgaggaag gagattgagg atgctgccca gtttgccacg gccgatcctg agccaccttt
     1681 ggaagagctg ggctaccaca tctactccag cgacccacct tttgaagttc gtggtgccaa
     1741 tcagtggatc aagtttaagt cagtcagtag ctccggaggg gatccaccgg tcgccaccat
     1801 ggtgagtgcg attaagccag acatgaggat caaactccgt atggaaggca acgtaaacgg
     1861 gcaccacttt gtgatcgacg gagatggtac aggcaagcct tatgagggaa aacagaccat
     1921 ggatcttgaa gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc
     1981 attccattac ggcaacaggg tattcgtgaa atatccagac aacatacaag actattttaa
     2041 gcagtcgttt cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat
     2101 ttgcaatgcc agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt
     2161 ttatggtacc aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga
     2221 gccctccact gagaaaatgt atgtgcgtga tggagtgctg acgggtgata ttgagatggc
     2281 tttgttgctt gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa
     2341 ggagaagggt gtcaagttac caggcgccca ctttgtggac cacgccattg agattttaag
     2401 ccatgacaaa gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt
     2461 gcctgacaat gccagacgat aagcggccgc gactctagat cataatcagc cataccacat
     2521 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2581 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2641 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2701 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
     2761 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     2821 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     2881 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2941 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     3001 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     3061 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     3121 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     3181 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3241 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3301 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     3361 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3421 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3481 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3541 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3601 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3661 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     3721 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3781 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3841 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     3901 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3961 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     4021 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     4081 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     4141 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     4201 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     4261 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     4321 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     4381 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     4441 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     4501 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     4561 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     4621 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     4681 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     4741 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     4801 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     4861 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     4921 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     4981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5161 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5221 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5281 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5341 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5461 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5641 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5701 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5761 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt
     5821 tat
//
