LOCUS       sequence-209013-        7407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 490 / 553, 
            Emission: 507 / 573.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7407)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dendra2-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..7407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1242
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     protein_bind    3061..3072
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    4135..4256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4172..4191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4226..4245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4263..4718)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4400..4421)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4612..4631
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4745..4849
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4851..5208
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4851..5042
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4874..4894)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5059..5194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5243..6037
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5243..6037
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5297..5316)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5907..5926
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6225..6244)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6269..6316
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6645..7233
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7134..7153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgaacac cccgggaatt aacctgatca aggaggacat gcgcgtgaag
      601 gtgcacatgg agggcaacgt gaacggccac gccttcgtga tcgagggcga gggcaagggc
      661 aagccctacg agggcaccca gaccgccaac ctgaccgtga aggagggcgc ccccctgccc
      721 ttcagctacg acatcctgac caccgccgtg cactacggca accgggtgtt caccaagtac
      781 cccgaggaca tccccgacta cttcaagcag agcttccccg agggctacag ctgggagcgc
      841 accatgacct tcgaggacaa gggcatctgc accatccgca gcgacatcag cctggagggc
      901 gactgcttct tccagaacgt gcgcttcaag ggcaccaact tcccccccaa cggccccgtg
      961 atgcagaaga agaccctgaa gtgggagccc agcaccgaga agctgcacgt gcgcgacggc
     1021 ctgctggtgg gcaacatcaa catggccctg ctgctggagg gcggcggcca ctacctgtgc
     1081 gacttcaaga ccacctacaa ggccaagaag gtggtgcagc tgcccgacgc ccacttcgtg
     1141 gaccaccgca tcgagatcct gggcaacgac agcgactaca acaaggtgaa gctgtacgag
     1201 cacgccgtgg cccgctacag ccccctgccc agccaggtgt ggtccggact cagatctggc
     1261 agcggtggag gcttggagga gccggaggcc gcgacgcgga cagccgcggc cgtggattgc
     1321 aaagacagac ccgggttccc agtgaagagg ttaatacaag cccgcttgcc cttcaaacgc
     1381 ctgaaccttg ttcccaagga gaaagttgag gaagacacat caccaaaagc agctgtggaa
     1441 agcaaagtcc cggatctaca attgtctttg ggcacttttg aaagccagtg tcacacaggc
     1501 tctcacgtag gcttgagtac aaagttggtc ggtggccagg gccccattga tagcttcttg
     1561 agggcaacaa tcaagccagt accgagtgtg gtcattatcg acctgacgga gaactgcagt
     1621 gacattccag acagccctga gggccacagt gaactaagtc ctgacactgc tggtgtggtc
     1681 accacagtgg aaggagctgc caagcagcag gagcactctg cagcagagct gtgtctccta
     1741 gagacccctt cagacatcac ttgtcacatg gaagaggagc ctgggagtcc aggagaccca
     1801 aagaggacgg gtgactgtca ggctggttca ctacagagct gtccggagct gaccccaggt
     1861 tctaggacat gccccaccaa ggagttatca agctggagca aggcagggga tctcctattt
     1921 atagagaaag tgcctgtggt ggtgctagag gacatcctgg ccactaagcc ctccattgcc
     1981 tctcttccca tgatgtccct ggataggagt gtcacttcag agagtgaaat actggagtcc
     2041 tgccccgagg atgactccat actgagccac tcctccacca actcctcttc tccaaccagc
     2101 tctcctgagg gaccatccac acccccagaa catcggggtg ggagaagctc cccctccacc
     2161 ccagcctgca gagttgccaa gaactttgtc aaaggctcca cagagaaggg caggagcaaa
     2221 ctgcacagag atagagaaca gcagagggaa gagaaggaaa agctgaggga agagatacga
     2281 cgagccaagg aggaggcacg gaagaagaag gaggaggaga aggagctgaa ggaaaaggag
     2341 cggagggaaa agcgggagaa agatgagaag gagaaggcgg agaagcagcg gctaaaggag
     2401 gaaaaacgca aggagcggca ggaagctctg gaggctaaac tggaggagaa gaggaagaag
     2461 gaggaagaga agcggttacg ggaggaagag aagcggttac gggaagaaga gaagcgcatt
     2521 aaggcagaaa aggcagagat cactcgattc ttccagaaac cgaagactcc gcaggctccc
     2581 aagaccctgg ccggttcctg tggtaagttt gccccctttg aaatcaaaga acacatggtg
     2641 ctggctccac ggtgccgagc tgccttggat caagacctgt gcgatcagct ggaccagctc
     2701 ctgcagcagc agagtgtggc aagcaccttc ctcagtgacc taaaaagccg gctgcccttg
     2761 agatcggggc ccacccgggt ctgtggccat gacacagaca tcatgaacag ggatgtggtc
     2821 attgtggaga gcagcaaggt ggacggtgtg tctgaaagga agaagtttgg ccgcatgaag
     2881 cttctgcagt tctcagagaa ccaccggcca gcatactggg ggacgtggaa caagaagaca
     2941 gccatcatcc gcccccggaa cccctgggcc caggacaaag accttcttga ctatgaggta
     3001 gacagtgacg atgagtggga agaggaggaa cctggggagt ctttgtccca cagtgagggg
     3061 gatgaggatg acgatgtggg ggaagatgag gatgaggacg atggcttctt tgtgccccat
     3121 gggtacttat ctgaagatga aggcgtgact gaggagtgtg cagaccctga gaaccacaaa
     3181 gtacaccaga agctgaaggc caaggagtgg gatgagctcc tagccaaggg caagcgcttt
     3241 cgtgtgctgc agcctgtgca cgtgggatgt gtatgggcat cagaggcagc caactgcacg
     3301 agttccgact tgaagctgct gcagcagttc actgcatgcc tgctggatgt ggcatcccct
     3361 gatgagccag aacccggggc ctccaggagg gaaaagaggg accagcatat cctggcccag
     3421 ttgcttccgc tgctacatgg caatgtgaat gggagcaagg tgatcatcca tgaattccag
     3481 gagcaatgcc gcaggggcct gctgaccctt ccaagtccca ccccacacct gcagatgccc
     3541 aacttggagg atgctgttgc tgtgccatcc aaggccaggc tgaagcgcct tatctctgag
     3601 aactctgcct atgagaagcg gcccaacttc cgtatgtgct ggtatgtgca cccagaggtg
     3661 ctcaagagct ttggccagga gtgcctgcca gtgccctgcc agtggaccta catcaccacc
     3721 atgccctcag cacccaggga agacagtggc agtgcttcta ctgaggggcc tggccagagc
     3781 acacctatgc tactcaagcg gaagccagcg gccaccatgt gcatcacgca gtttatgaag
     3841 aagcggcgct atgatggaca ggttgggtct ggggacatgg atggcttcca ggcagacaca
     3901 gaagaggatg aagaggatga cacagactgt atgatcatag atgtgccaga tgttgggagt
     3961 gacgtgtcag aagctcccat acctgctccc acactttgca agtgaggatc caccggatct
     4021 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     4081 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     4141 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4201 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     4261 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     4321 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     4381 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     4441 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     4501 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     4561 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     4621 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     4681 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     4741 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4801 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     4861 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     4921 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     4981 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5041 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5101 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5161 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     5221 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     5281 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     5341 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     5401 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     5461 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     5521 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     5581 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     5641 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     5701 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     5761 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     5821 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     5881 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     5941 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     6001 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     6061 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     6121 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     6181 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     6241 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     6301 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     6361 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     6421 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     6481 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6541 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6601 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6661 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6721 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6781 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6841 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6901 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6961 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7021 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7081 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7141 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7201 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7261 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7321 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
     7381 agttcatagc ccatatatgg agttccg
//
