LOCUS       sequence-209021-        1378 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human beta-actin fused with thymosin beta and a His tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Hatano T, Alioto S, Roscioli E, Palani S, Clarke ST, Kamnev A, 
            Hernandez-Fernaud JR, Sivashanmugam L, Chapa-Y-Lazo B, Jones AME, 
            Robinson RC, Sampath K, Mishima M, McAinsh AD, Goode BL, 
            Balasubramanian MK
  TITLE     Rapid production of pure recombinant actin isoforms in Pichia 
            pastoris.
  JOURNAL   J Cell Sci. 2018 Mar 13. pii: jcs.213827. doi: 10.1242/jcs.213827.
  PUBMED    29535210
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2018 Mar 13. pii: jcs.213827. doi: 10.1242/jcs.213827."
COMMENT     Sequence Label: pPICZc-HsACTB*-thymosinB-8His
FEATURES             Location/Qualifiers
     source          1..1378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1322..1342
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1346..1369
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 aaacgaggaa ttcgccacca tggatgatga tatcgccgcg ctcgtcgtcg acaacggctc
       61 cggcatgtgc aaggccggct tcgcgggcga cgatgccccc cgggccgtct tcccctccat
      121 cgtggggcgc cccaggcacc agggcgtgat ggtgggcatg ggtcagaagg attcctatgt
      181 gggcgacgag gcccagagca agagaggcat cctcaccctg aagtacccca tcgagcacgg
      241 catcgtcacc aactgggacg acatggagaa aatctggcac cacaccttct acaatgagct
      301 gcgtgtggct cccgaggagc accccgtgct gctgaccgag gcccccctga accccaaggc
      361 caaccgcgag aagatgaccc agatcatgtt tgagaccttc aacaccccag ccatgtacgt
      421 tgctatccag gctgtgctat ccctgtacgc ctctggccgt accactggca tcgtgatgga
      481 ctccggtgac ggggtcaccc acactgtgcc catctacgag gggtatgccc tcccccatgc
      541 catcctgcgt ctggacctgg ctggccggga cctgactgac tacctcatga agatcctcac
      601 cgagcgcggc tacagcttca ccaccacggc cgagcgggaa atcgtgcgtg acattaagga
      661 gaagctgtgc tacgtcgccc tggacttcga gcaagagatg gccacggctg cttccagctc
      721 ctccctggag aagagctacg agctgcctga cggccaggtc atcaccattg gcaatgagcg
      781 gttccgctgc cctgaggcac tcttccagcc ttccttcctg ggcatggagt cctgtggcat
      841 ccacgaaact accttcaact ccatcatgaa gtgtgacgtg gacatccgca aagacctgta
      901 cgccaacaca gtgctgtctg gcggcaccac catgtaccct ggcattgccg acaggatgca
      961 gaaggagatc actgccctgg cacccagcac aatgaagatc aagatcattg ctcctcctga
     1021 gcgcaagtac tccgtgtgga tcggcggctc catcctggcc tcgctgtcca ccttccagca
     1081 gatgtggatc agcaagcagg agtatgacga gtccggcccc tccatcgtcc accgcaaatg
     1141 cttcgctagc cgtggcggca gcggtgggag ctctggcggt agcgcttccg ataagccaga
     1201 tatggcggaa atcgaaaagt tcgataaatc taaactgaag aaaactgaga ctcaagaaaa
     1261 gaacccgctg ccgagcaagg aaaccatcga gcaggagaag caggcgggtg aatccggtac
     1321 cgaaaattta tattttcaag ggccccacca ccaccaccac caccaccacg aagaataa
//
