LOCUS       sequence-209024-        9985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fibroblasts specific inducible Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9985)
  TITLE     Cre-inducible plasmids
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Col1A2:TFPnls-T2a-mErt-Cre-Ert
FEATURES             Location/Qualifiers
     source          1..9985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     649..668
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        649..667
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(682..693,694..881)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2453..2469)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2454..2470)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          2941..3073
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3056..3075
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     3170..3189
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3237..3246
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3243..3950
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             3975..3995
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3999..4019
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4023..4043
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4065..4118
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5073..6095
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             6108..7043
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6156..7049
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(7133..7152)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7198..7253
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7252..7271)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    join(7598..7735,7736..7747)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7774..7794)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7774..7792)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7836..7852)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7836..7852)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7849..7871)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7860..7876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7884..7890,7891..7908,7909..7914))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7929..7950
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8067..8084)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8238..8826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8318..8337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8997..9788,9789..9857))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9620..9639
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9858..9962)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgtagggat aacagggtaa tgcgcgcgta atacgactca
      661 ctatagggcg aattgggtac ccagaggtgt aaagtacttg agtaatttta cttgattact
      721 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
      781 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
      841 ttatttacag tcaaaaagta cttatttttt ggagatcact tgggcccggc tcgagcatgc
      901 acgcgtgaat tccccaattt ttctctgtga atctcctccc acacacacac cctttttctc
      961 ctgggcttca gcatccatcg taactagtta gctccactca cattatccac tattttactc
     1021 aaaatgcgag gattactctt tgctttatct tcaagagaag tggagagagc cagacaaagg
     1081 aagaagaggg agaacaggag aggagatgag gggggagatt agaatggggt aagggagaga
     1141 acaggagctg agaagagcgg gagaggggga gagaaggatt cagacattct aaactctctt
     1201 cctgagtgct atttaaagca gtttccaacc tggagagaat gtaatgcaca gttcctccat
     1261 gtgtttagtt aaggacctca tgagtcttgt ggatttctcc acaacctaag tggttattat
     1321 gctgacccga gggtgtgcca tttggtggga cacttcatct tattgtccct gtttatgtgt
     1381 agcaagcaag ttcagttctg taagacactg attagagggg caggaaaatt gttctctact
     1441 gtgccttgtg tacagcaaga agctgtccag tgttgttcac ataattgaat gtgtggaact
     1501 tcccagcttg caggtggggt cttcagttct acctcagtct ctttgggaga ctaccacaac
     1561 tcaccttgag taattagaaa acagcacaga agtggctgcg cttcctgaag actacaaagg
     1621 agctgttgcc tgcttctctt aactccatct tgggctcagc tgacccaaaa gcggctgtct
     1681 ttgtttacac cagatttcaa ctttgcaggc aatcttacta agcagaggga ggcagtgggg
     1741 gatggtattg ggggtgagtt acttagtcca agtcccagag ctgagggtga gggagatgct
     1801 gtactttctt accagctgtt atgcatcaag aagaggcagg cttgtgtaac cctttcttct
     1861 cctgcacgtt tcaagcaaaa gccaggcact cactgagttt tgtaaagatt ttcccgtgaa
     1921 gacatacaca cagcgtaatt ccatgcaata atttctgtgt aaagccctta gtgcagtgta
     1981 ccattgtttg tgtctgggtc ttgaattgtc acaattaagt gtgagcacac acacaatgtg
     2041 ctctttagcc aaagtcataa cactgctacc tgcttccctt tgaaagaaaa cagccatctt
     2101 ctcaggaaat gtaagggggc aaggggtggg ggttaggggg aaggacaaga ttgtaaaaag
     2161 ccaaaagcct agggcttgga gctgctttgg ccaagcctag aatctattta aatgcccttc
     2221 cctgaagggt taagtgtgga acacctccca ggaaatgaca aatggccaga gttctgccca
     2281 ctcccttccc cccatcttcc agacatcaaa tacttaggaa ctgtttggct tgaaatggtt
     2341 cctgagactc atactcacag aacacattct ttttactctc ctcccccagc ccagtacagg
     2401 ctggaggtgg ctggcggggg taggggggga tgggtgaagg gggcaagctt atcgataccg
     2461 tcgacctcga ggttctagag cttggatctg taaagagccc acgtaggtgt cctaaagtgc
     2521 ttccaaactt ggcaagggcg agagagggcg ggtggctggg gagggcggag gtatgcagac
     2581 agggagtcag agttccccct cgaaagcctc aaaagtgtcc acgtcctcaa aaagaatgga
     2641 accaatttaa gaagccccgt agccacgtcc ctcccccctc ggctcccccc cctgctcccc
     2701 cgcagtctcc tcccagcact gagtcccggg cccctagccc tagccctccc attggtggag
     2761 acgtttttgg aggcaccctc cggctgggga aacttttccc atataaataa ggcaggtctg
     2821 ggctttatta ttttagcacc acggcagcag gaggtttcga ctaagttgga gggaacggtc
     2881 cacgattgcc aaggaattcg aattttaagc tgcagaagtt ggtcgtgagg cactgggcag
     2941 gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga
     3001 cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc
     3061 tttctctcca caggtgtcca ctcccagttc aattacagct cttaaaattc cgccactaga
     3121 gcctctgcta accatgttca tgccttcttc tctttcctac agctcctggg caacgtgctg
     3181 gttattgtgc tgtctcatca ttttggcaaa gaattattcc gctagcgcta ccggtcgcca
     3241 ccatggtgag caagggcgag gagaccacaa tgggcgtaat caagcccgac atgaagatca
     3301 agctgaagat ggagggcaac gtgaatggcc acgccttcgt gatcgagggc gagggcgagg
     3361 gcaagcccta cgacggcacc aacaccatca acctggaggt gaaggaggga gcccccctgc
     3421 ccttctccta cgacattctg accaccgcgt tcgcctacgg caacagggcc ttcaccaagt
     3481 accccgacga catccccaac tacttcaagc agtccttccc cgagggctac tcttgggagc
     3541 gcaccatgac cttcgaggac aagggcatcg tgaaggtgaa gtccgacatc tccatggagg
     3601 aggactcctt catctacgag atacacctca agggcgagaa cttccccccc aacggccccg
     3661 tgatgcagaa gaagaccacc ggctgggacg cctccaccga gaggatgtac gtgcgcgacg
     3721 gcgtgctgaa gggcgacgtc aagcacaagc tgctgctgga gggcggcggc caccaccgcg
     3781 ttgacttcaa gaccatctac agggccaaga aggcggtgaa gctgcccgac tatcactttg
     3841 tggaccaccg catcgagatc ctgaaccacg acaaggacta caacaaggtg accgtttacg
     3901 agagcgccgt ggcccgcaat tccaccgacg gcatggacga gctgtacaag tccggactca
     3961 gatctcgagc tgatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtag
     4021 atccaaaaaa gaagagaaag gtaggatcca ccggatctag acccgagggc agaggaagtc
     4081 ttctaacatg cggtgacgtg gaggagaatc ccggcccact agtcatggcc ggcgatatga
     4141 gagccgctaa cctgtggccc tccccactga tgatcaagag aagcaagaag aactccctgg
     4201 ccctgagcct gaccgctgat cagatggtgt ccgccctgct ggacgccgag ccccctatcc
     4261 tgtacagcga gtacgacccc accaggccct tcagcgaggc cagcatgatg ggactgctga
     4321 caaacctggc cgatcgcgaa ctggtgcata tgatcaattg ggccaagcgc gtgcccggat
     4381 tcgtggacct gacactgcac gaccaggtgc acctgctgga atgcgcttgg ctggaaattc
     4441 tgatgatcgg cctcgtgtgg cggagcatgg aacatcctgt gaagctgctg ttcgccccca
     4501 atctgctgct ggaccgcaac cagggcaaat gcgtggaagg gatggtcgaa atctttgata
     4561 tgctgctcgc caccagcagc aggttcagaa tgatgaacct ccagggcgaa gagttcgtct
     4621 gcctgaagtc catcatcctg ctgaacagcg gcgtgtacac cttcctgtcc tctaccctga
     4681 agtccctcga ggaaaaggat cacatccaca gggtgctgga taagatcacc gataccctga
     4741 tccatctcat ggctaaggcc ggactcacac tccagcagca gcatcagaga ctcgctcagc
     4801 tgctgctgat cctgagccac atccgccaca tgtccaacaa gggaatggaa cacctctact
     4861 ctatgaagtg taaaaacgtc gtccccctgt acgatctgct gctcgaggcc gccgatgccc
     4921 acagactgca cgcccctaca agcagaggcg gcgctagcgt ggaagagaca gaccagtccc
     4981 acctggccac cgccggcagc acaagcagcc acagcctgca gaagtactat atcaccggcg
     5041 aggccgaggg cttccccgct acagctgtcg acaatttact gaccgtacac caaaatttgc
     5101 ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg gacatgttca
     5161 gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt
     5221 gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct gaagatgttc
     5281 gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc cagcaacatt
     5341 tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt gacagcaatg
     5401 ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa
     5461 aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc atggaaaata
     5521 gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat aacaccctgt
     5581 tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact gacggtggga
     5641 gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt gtagagaagg
     5701 cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct ggtgtagctg
     5761 atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg
     5821 ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact catcgattga
     5881 tttacggcgc taaggatgac tccggtcaga gatacctggc ctggtctgga cacagtgccc
     5941 gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag atcatgcaag
     6001 ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg gatagtgaaa
     6061 caggggcaat ggtgcgcctg ctggaagatg gcgatctcga gccatctgct ggagacatga
     6121 gagctgccaa cctttggcca agcccgctca tgatcaaacg ctctaagaag aacagcctgg
     6181 ccttgtccct gacggccgac cagatggtca gtgccttgtt ggatgctgag ccccccatac
     6241 tctattccga gtatgatcct accagaccct tcagtgaagc ttcgatgatg ggcttactga
     6301 ccaacctggc agacagggag ctggttcaca tgatcaactg ggcgaagagg gtgccaggct
     6361 ttgtggattt gaccctccat gatcaggtcc accttctaga atgtgcctgg ctagagatcc
     6421 tgatgattgg tctcgtctgg cgctccatgg agcacccagt gaagctactg tttgctccta
     6481 acttgctctt ggacaggaac cagggaaaat gtgtagaggg catggtggag atcttcgaca
     6541 tgctgctggc tacatcatct cggttccgca tgatgaatct gcagggagag gagtttgtgt
     6601 gcctcaaatc tattattttg cttaattctg gagtgtacac atttctgtcc agcaccctga
     6661 agtctctgga agagaaggac catatccacc gagtcctgga caagatcaca gacactttga
     6721 tccacctgat ggccaaggca ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc
     6781 tcctcctcat cctctcccac atcaggcaca tgagtaacaa aggcatggag catctgtaca
     6841 gcatgaagtg caagaacgtg gtgcccctct atgacctgct gctggaggcg gcggacgccc
     6901 accgcctaca tgcgcccact agccgtggag gggcatccgt ggaggagacg gaccaaagcc
     6961 acttggccac tgcgggctct acttcatcgc attccttgca aaagtattac atcacggggg
     7021 aggcagaggg tttccctgcc acagcttaag atatcactcc tcaggtgcag gctgcctatc
     7081 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
     7141 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     7201 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     7261 ggacatatgg gagggcaaat catttaaaac atcagaatga gcatttggtt tagagtttgg
     7321 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     7381 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     7441 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     7501 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     7561 ctcttctctt atgaagatcc ctcgaccagc ggccgctaat actcaagtac aattttaatg
     7621 gagtactttt ttacttttac tcaagtaaga ttctagccag atacttttac ttttaattga
     7681 gtaaaatttt ccctaagtac ttgtactttc acttgagtaa aatttttgag tactttttac
     7741 acctctgccg cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgcat
     7801 taccctgtta tccctacgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7861 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7921 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7981 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8041 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8101 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8161 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8221 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8281 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8341 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8401 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8461 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8521 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8581 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8641 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     8701 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8761 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8821 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8881 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8941 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9001 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9061 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9121 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9181 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9241 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9301 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9361 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9421 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9481 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9541 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9601 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9661 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9721 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9781 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9841 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9901 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9961 cgcacatttc cccgaaaagt gccac
//
