LOCUS       sequence-209026-        8597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  knock in bait for Axolotl Pax7 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Fei JF, Schuez M, Knapp D, Taniguchi Y, Drechsel DN, Tanaka EM
  TITLE     Efficient gene knockin in axolotl and its use to test the role of 
            satellite cells in limb regeneration.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):12501-12506. doi: 
            10.1073/pnas.1706855114. Epub 2017 Oct 31.
  PUBMED    29087939
REFERENCE   2  (bases 1 to 8597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1706855114"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2017-11-21- 21"; volume: "114"; issue: "47"; pages: 
            "12501-12506"
COMMENT     Sequence Label: pGEMT-Pax7bait-P2a-GAP43-Cherry-T2a-mERt-Cre-Ert
FEATURES             Location/Qualifiers
     source          1..8597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..38
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     30..47
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     31..47
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1455..1511
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1572..2279
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1717..1735)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1985..2004
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2280..2333
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3282..4304
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             4317..5252
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4365..5258
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(5342..5361)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5407..5462
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5461..5480)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        complement(5792..5810)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5793..5810)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5828..5844)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5828..5844)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5841..5863)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5852..5868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5876..5882,5883..5900,5901..5906))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5921..5942
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6059..6076)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6230..6818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6310..6329)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6989..7780,7781..7849))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7612..7631
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7850..7954)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8032..8487)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8169..8190)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8381..8400
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg
       61 actcactata gacgcgtatg gcttgtctcc ccggagctgt ccccagaatg atgcggccag
      121 gaccagggca gaactaccca cggaccggtt tcccactaga agtgtccact cctctgggcc
      181 agggcagagt caaccaactg gggggagtgt tcattaacgg gcgcccgttg cccaaccaca
      241 tccgccacaa gatcgtggag atggcacacc acggcattcg gccctgcgtg atttcacggc
      301 agctacgtgt gtcccacggc tgcgtgtcca agatcctgtg ccggtaccag gagacggggt
      361 ccatccggcc cggagccatc ggaggcagca agcccaggca ggtggccact cctgacgtgg
      421 agaagaagat tgaggaatat aagagagaga accccgggat gttcagctgg gagatcaggg
      481 acaggctcct aaaggatgga cactgcgaca ggagcacggt gccctcaggt aaccggctgg
      541 acgaaggttc agatgttgag tcagagccag acttgccctt gaaacgcaag cagcgacgca
      601 gccgcaccac cttcactgct gagcagctgg aggagttgga gaaggccttt gagagaacgc
      661 actacccaga tatctacacc cgtgaagagt tggcgcagag aaccaagctc accgaggcga
      721 gagtacaggt gtggttcagc aatcgtagag caaggtggcg taaacaggca ggagccaatc
      781 aactggcagc tttcaatcat ctcctgcccg gcggatttcc acccacagga atgccaagtt
      841 tgccacccta tcagcttcca gactccggct atgcaagtgc cctagcacaa gacgggggaa
      901 gcacagtgca cagacctcag cccctgccac cctcctccat gcaccagggt ggattgtcag
      961 ctgctgatgc cagttcagcc tacggacccc gacacagctt caccagctat tcggacagct
     1021 tcatgaaccc aggcgggcca actaaccaca tgaatccggt cagcaatggg ctgtctccac
     1081 aggtgatgag catcctcagc aaccctagtg gagtgcagtc ccagtcccag gatttctcca
     1141 tctcccctct gcatggtggc ttggacactt cgaactccat ctcggcaagc tgcagccagc
     1201 ggagcgactc catcaagtca gtcgatagcc tcgccacctc gcagtcctac tgtcctccga
     1261 cctacagcac caccggctac agcgtggatc ctgtggccag ctaccaatat gggcaatacg
     1321 ggcaaactgc tgtagactac ttggctaaga acgtgagctt gtcgacacag aggaggatga
     1381 agctcgggga ccactcagcc gtgttgggtt tgcttcctgt ggagacggga caagcatacg
     1441 catgcggaag cggagctact aacttcagcc tgctgaagca ggctggagac gtggaggaga
     1501 accctggacc tatgctgtgc tgtatgagaa gaaccaaaca ggttgaaaag aatgatgagg
     1561 accaaaagat catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     1621 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     1681 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     1741 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     1801 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     1861 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     1921 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     1981 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     2041 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     2101 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     2161 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     2221 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg
     2281 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatggccg
     2341 gcgatatgag agccgctaac ctgtggccct ccccactgat gatcaagaga agcaagaaga
     2401 actccctggc cctgagcctg accgctgatc agatggtgtc cgccctgctg gacgccgagc
     2461 cccctatcct gtacagcgag tacgacccca ccaggccctt cagcgaggcc agcatgatgg
     2521 gactgctgac aaacctggcc gatcgcgaac tggtgcatat gatcaattgg gccaagcgcg
     2581 tgcccggatt cgtggacctg acactgcacg accaggtgca cctgctggaa tgcgcttggc
     2641 tggaaattct gatgatcggc ctcgtgtggc ggagcatgga acatcctgtg aagctgctgt
     2701 tcgcccccaa tctgctgctg gaccgcaacc agggcaaatg cgtggaaggg atggtcgaaa
     2761 tctttgatat gctgctcgcc accagcagca ggttcagaat gatgaacctc cagggcgaag
     2821 agttcgtctg cctgaagtcc atcatcctgc tgaacagcgg cgtgtacacc ttcctgtcct
     2881 ctaccctgaa gtccctcgag gaaaaggatc acatccacag ggtgctggat aagatcaccg
     2941 ataccctgat ccatctcatg gctaaggccg gactcacact ccagcagcag catcagagac
     3001 tcgctcagct gctgctgatc ctgagccaca tccgccacat gtccaacaag ggaatggaac
     3061 acctctactc tatgaagtgt aaaaacgtcg tccccctgta cgatctgctg ctcgaggccg
     3121 ccgatgccca cagactgcac gcccctacaa gcagaggcgg cgctagcgtg gaagagacag
     3181 accagtccca cctggccacc gccggcagca caagcagcca cagcctgcag aagtactata
     3241 tcaccggcga ggccgagggc ttccccgcta cagctgtcga caatttactg accgtacacc
     3301 aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg
     3361 acatgttcag ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt
     3421 gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg
     3481 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
     3541 agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg
     3601 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
     3661 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca
     3721 tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata
     3781 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg
     3841 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
     3901 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
     3961 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
     4021 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
     4081 atcgattgat ttacggcgct aaggatgact ccggtcagag atacctggcc tggtctggac
     4141 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
     4201 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
     4261 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg
     4321 gagacatgag agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga
     4381 acagcctggc cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc
     4441 cccccatact ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg
     4501 gcttactgac caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg
     4561 tgccaggctt tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc
     4621 tagagatcct gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt
     4681 ttgctcctaa cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga
     4741 tcttcgacat gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg
     4801 agtttgtgtg cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca
     4861 gcaccctgaa gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag
     4921 acactttgat ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc
     4981 tggcccagct cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc
     5041 atctgtacag catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg
     5101 cggacgccca ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg
     5161 accaaagcca cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca
     5221 tcacggggga ggcagagggt ttccctgcca cagcttaaga tatcactcct caggtgcagg
     5281 ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag
     5341 atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt
     5401 ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct
     5461 cactcggaag gacatatggg agggcaaatc atttaaaaca tcagaatgag catttggttt
     5521 agagtttggc aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag
     5581 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     5641 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     5701 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     5761 tagctgtccc tcttctctta tgaagatcta ttctatagtg tcacctaaat agcttggcgt
     5821 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5881 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5941 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6001 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6061 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6121 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6181 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6241 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6301 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6361 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6421 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6481 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6541 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6601 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6661 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6721 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6781 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6841 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6901 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6961 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7021 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7081 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7141 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7201 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7261 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7321 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7381 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7441 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7501 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7561 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7621 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7681 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7741 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7801 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7861 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7921 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7981 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     8041 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     8101 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     8161 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     8221 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     8281 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     8341 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     8401 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     8461 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     8521 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     8581 aaggcgatta agttggg
//
