LOCUS       sequence-209029-        7860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, hShh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Nacu E, Gromberg E, Oliveira CR, Drechsel D, Tanaka EM
  TITLE     FGF8 and SHH substitute for anterior-posterior tissue interactions 
            to induce limb regeneration.
  JOURNAL   Nature. 2016 May 19;533(7603):407-10. doi: 10.1038/nature17972.
  PUBMED    27120163
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-05-19- 19"; volume: "533"; issue: "7603"; 
            pages: "407-10"
COMMENT     Sequence Label: pOEM1_pCMV:hShh-pPH:vsvged
FEATURES             Location/Qualifiers
     source          1..7860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     3'UTR           236..608
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        709..1012
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1013..1216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1167..1187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1255..1264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2733..2753)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3151..3162)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3397..3531
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3434..3453)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3488..3507
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3644..3676)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(3872..3931)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(3994..4085)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4052..4069)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4171..4776
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(4814..4938)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(5548..5567)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5549..5567)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5578..5595)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5578..5594)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5587..5609)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        5910..6014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6015..6083,6084..6875)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6233..6252)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7046..7634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7535..7554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7727..7744
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agctcaacta caaatttgat cgcggacgtg ttagccgaca caattaatag gcgtcgtgtg
       61 gctatggcaa aatcgtcttc ggaagcaact tctaacgacg agggttggga cgacgacgat
      121 aatcggccta ataaagctaa cacgcccgat gttaaatatg tccaagcgtt atttaacgtg
      181 tttacgtcga gtcaattgta cactaacgac agtgatgaaa gaaatacaaa agcgcataat
      241 attttgaacg acgtcgaacc tttattacaa aacaaaacac aaacgaatat cgacaaagct
      301 agattgctgc tacaagattt ggcaagtttt gtggcgttga gcgaaaatcc attagatagt
      361 ccagccatcg gttcggaaaa acaacccttg tttgaaacta atcgaaacct attttacaaa
      421 tctattgagg atttaatatt taaattcaga tataaagacg ctgaaaatca tttgattttc
      481 gctctaacat accaccctaa agattataaa tttaatgaat tattaaaata cgtacaacaa
      541 ttgtctgtaa atcaacaacg cacagaatct agcgcttaat aaatgtacta ataacaatgt
      601 atcgtgtttt attaattaag atctgcggat gcaggctccg ccatgcatta gttattaata
      661 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      721 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      781 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      841 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      901 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      961 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     1021 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     1081 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     1141 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     1201 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
     1261 atgggcgaga tgctgctgct ggcgagatgt ctgctgctag tcctcgtctc ctcgctgctg
     1321 gtatgctcgg gactggcgtg cggaccgggc agggggttcg ggaagaggag gcaccccaaa
     1381 aagctgaccc ctttagccta caagcagttt atccccaatg tggccgagaa gaccctaggc
     1441 gccagcggaa ggtatgaagg gaagatctcc agaaactccg agcgatttaa ggaactcacc
     1501 cccaattaca accccgacat catatttaag gatgaagaaa acaccggagc ggacaggctg
     1561 atgactcaga ggtgtaagga caagttgaac gctttggcca tctcggtgat gaaccagtgg
     1621 ccaggagtga aactgcgggt gaccgagggc tgggacgaag atggccacca ctcagaggag
     1681 tctctgcact acgagggccg cgcagtggac atcaccacgt ctgaccgcga ccgcagcaag
     1741 tacggcatgc tggcccgcct ggcggtggag gccggcttcg actgggtgta ctacgagtcc
     1801 aaggcacata tccactgctc ggtgaaagca gagaactcgg tggcggccaa atcgggaggc
     1861 tgcttcccgg gctcggccac ggtgcacctg gagcagggcg gcaccaagct ggtgaaggac
     1921 ctgagccccg gggaccgcgt gctggcggcg gacgaccagg gccggctgct ctacagcgac
     1981 ttcctcactt tcctggaccg cgacgacggc gccaagaagg tcttctacgt gatcgagacg
     2041 cgggagccgc gcgagcgcct gctgctcacc gccgcgcacc tgctctttgt ggcgccgcac
     2101 aacgactcgg ccaccgggga gcccgaggcg tcctcgggct cggggccgcc ttccgggggc
     2161 gcactggggc ctcgggcgct gttcgccagc cgcgtgcgcc cgggccagcg cgtgtacgtg
     2221 gtggccgagc gtgacgggga ccgccggctc ctgcccgccg ctgtgcacag cgtgacccta
     2281 agcgaggagg ccgcgggcgc ctacgcgccg ctcacggccc agggcaccat tctcatcaac
     2341 cgggtgctgg cctcgtgcta cgcggtcatc gaggagcaca gctgggcgca ccgggccttc
     2401 gcgcccttcc gcctggcgca cgcgctcctg gctgcactgg cgcccgcgcg cacggaccgc
     2461 ggcggggaca gcggcggcgg ggaccgcggg ggcggcggcg gcagagtagc cctaaccgct
     2521 ccaggtgctg ccgacgctcc gggtgcgggg gccaccgcgg gcatccactg gtactcgcag
     2581 ctgctctacc aaataggcac ctggctcctg gacagcgagg ccctgcaccc gctgggcatg
     2641 gcggtcaagt ccagctgagc ggccgctaga cgcgtcgaca atcaacctct ggattacaaa
     2701 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2761 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2821 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2881 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2941 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3001 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3061 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     3121 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3181 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3241 cggatctccc tttgggccgc ctccccgaag cttgtcgaga agtactagag gatcataatc
     3301 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     3361 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     3421 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     3481 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg
     3541 gcgcgccgtg atgcgtattt ctgaatcttt gtaaaatagc acacaagact ccaacgcgtt
     3601 tggcgtttta ttttcttgct cgactctagt ttattaggcc ttactttcca agtcggttca
     3661 tctctatgtc tgtataaatc tgtcttttct tggtgtgctt taatttaatg caaagatgga
     3721 taccaactcg gagaaccaag aatagtccaa tgattaaccc tatgataaag aaaaaagagg
     3781 caatagagct tttccaacta ctgaaccaac cttctacaag ctcgattgga tttttggata
     3841 gcccagtatc accccggggt acctgcagat ccgcaaaggc agaatgcgcc gccgccgcca
     3901 aaagcacata taaaacaata gcgcttacca tcttgcttgt gtgttcctta ttgaagcctt
     3961 ggtgtgactg atttactagt agcatagatc cgtatttata ggttttttta ttacaaaact
     4021 gttacgaaaa cagtaaaata cttatttatt tgcgagatgg ttatcatttt aattatctcc
     4081 atgatccagc caaccactag aactatagct agagtcctgg gcgaacaaaa tggtggcagc
     4141 ttgtgatatt agtttgtgcg tctcattaca atggctgtta tttttaacaa caaacaactg
     4201 ctcgcagaca atagtataga aaagggaggt gaactgtttt tgtttaacgg ttcgtacaac
     4261 attttggaaa gttatgttaa tccggtgctg ctaaaaaatg gtgtaattga actagaagaa
     4321 gctgcgtact atgccggcaa catattgtac aaaaccgacg atcccaaatt cattgattat
     4381 ataaatttaa taattaaagc aacacactcc gaagaactac cagaaaatag cactgttgta
     4441 aattacagaa aaactatgcg cagcggtact atacacccca ttaaaaaaga catatatatt
     4501 tatgacaaca aaaaatttac tctatacgat agatacatat atggatacga taataactat
     4561 gttaattttt atgaggagaa aaatgaaaaa gagaaggaat acgaagaaga agacgacaag
     4621 gcgtctagtt tatgtgaaaa taaaattata ttgtcgcaaa ttaactgtga atcatttgaa
     4681 aatgatttta aatattacct cagcgattat aactacgcgt tttcaattat agataatact
     4741 acaaatgttc ttgttgcgtt tggtttgtat cgttaataaa aaacaaattt agcatttata
     4801 attgttttat tattcaataa ttacaaatag gattgagacc cttgcagttg ccagcaaacg
     4861 gacagagctt gtccacagtt ttcatttttt ggcagtttgg tggtttatac acatcttggg
     4921 atttgattaa aagatgcatg acttcaccca cacacttgga gtcgtggttg tagaaatgcg
     4981 tcagagcctt gattaaacat cggttttcac acttggcaca cgaaaccacg tttgcaatgt
     5041 aacagtttag tataaacctc ttcctgtaca taccgccgcg cggtacattg ttttctttca
     5101 aatcgttcaa tatttttttt atacacggag gcatattaat cttgttgttc agcatgtcca
     5161 cgacattttt ctttccttct acgcattctt tcatacaaat gtttctcttg ctgcgttttt
     5221 taaaattgga atttttaatt tcattaatag tgggcggagc cgttaacagc atgtacaatc
     5281 gcagtccgga aattttgacc aaaaccccgc cattgtagaa taccgtgtat ggagttaggg
     5341 tcaatttatc aataaaatca tcgggatcaa ttaaatatgt agctttcttg tctaaacatg
     5401 acgctctaat gagcggactc cacacgttat acgatgcatt cgccatactt cgcggcttct
     5461 cgcaccagcg gtcctgattc gactaacggt cgattggatg gcttaatagt tttaacgtca
     5521 cggccttgac ggccttccgc caattcgccc tatagtgagt cgtattacgt cgcgctcact
     5581 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5641 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgaaacgcc
     5701 cttcccaaca gttgcgcagc ctgaatggcg aatgggagcg ccctgtagcg gccactcaac
     5761 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5821 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5881 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5941 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6001 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6061 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6121 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6181 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6241 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6301 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6361 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6421 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6481 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6541 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6601 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6661 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6721 gtgagcgtgg ttctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6781 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6841 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6901 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6961 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7021 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7081 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7141 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7201 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7261 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7321 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7381 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7441 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7501 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7561 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7621 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7681 cttttgctca ttaggcaccc caggctttac ccgaacgacc gagcgcagcg agtcagtgag
     7741 cgaggaagcg gagagcgccc aatacgcaag gaaacagcta tgaccatgtt aatgcagctg
     7801 gcacgacagg tttcccgact ggaaagcggg cagtgagcgg aaggcccatg aggcccaaaa
//
