LOCUS       sequence-209030-        7691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, hShh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Wagner I, Wang H, Weissert PM, Straube WL, Shevchenko A, Gentzel M, 
            Brito G, Tazaki A, Oliveira C, Sugiura T, Shevchenko A, Simon A, 
            Drechsel DN, Tanaka EM
  TITLE     Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of 
            Dedifferentiating Muscle Cells during Newt Limb Regeneration.
  JOURNAL   Dev Cell. 2017 Mar 27;40(6):608-617.e6. doi: 
            10.1016/j.devcel.2017.03.002.
  PUBMED    28350991
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.03"; journalName: "Dev Cell"; date: 
            "2017-03-27- 27"; volume: "40"; issue: "6"; pages: "608-617.e6"
COMMENT     Sequence Label: pOEM1_pCMV:bmp4-pPH:vsvged
FEATURES             Location/Qualifiers
     source          1..7691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     692..709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           1061..1433
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        1534..1837
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1838..2041
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1992..2012
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(3389..3409)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3807..3818)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4053..4187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4090..4109)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4144..4163
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(4300..4332)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(4528..4587)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(4650..4741)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4708..4725)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4827..5432
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(5470..5594)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(6204..6223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6205..6223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6234..6251)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6234..6250)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6243..6265)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        6566..6670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6671..6739,6740..7531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6889..6908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcattagg
      661 caccccaggc tttacccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggagag
      721 cgcccaatac gcaaggaaac agctatgacc atgttaatgc agctggcacg acaggtttcc
      781 cgactggaaa gcgggcagtg agcggaaggc ccatgaggcc caaaaagctc aactacaaat
      841 ttgatcgcgg acgtgttagc cgacacaatt aataggcgtc gtgtggctat ggcaaaatcg
      901 tcttcggaag caacttctaa cgacgagggt tgggacgacg acgataatcg gcctaataaa
      961 gctaacacgc ccgatgttaa atatgtccaa gcgttattta acgtgtttac gtcgagtcaa
     1021 ttgtacacta acgacagtga tgaaagaaat acaaaagcgc ataatatttt gaacgacgtc
     1081 gaacctttat tacaaaacaa aacacaaacg aatatcgaca aagctagatt gctgctacaa
     1141 gatttggcaa gttttgtggc gttgagcgaa aatccattag atagtccagc catcggttcg
     1201 gaaaaacaac ccttgtttga aactaatcga aacctatttt acaaatctat tgaggattta
     1261 atatttaaat tcagatataa agacgctgaa aatcatttga ttttcgctct aacataccac
     1321 cctaaagatt ataaatttaa tgaattatta aaatacgtac aacaattgtc tgtaaatcaa
     1381 caacgcacag aatctagcgc ttaataaatg tactaataac aatgtatcgt gttttattaa
     1441 ttaagatctg cggatgcagg ctccgccatg cattagttat taatagtaat caattacggg
     1501 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1561 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1621 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1681 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1741 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1801 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     1861 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1921 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     1981 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2041 tggtttagtg aaccgtcaga tccgctagcg ctaccggtgc cgccaccatg attcctggta
     2101 accgaatgct gatggtcgtt ttattatgcc aagtcctgct aggaggcgcg agccatgcta
     2161 gtttgatacc tgagacgggg aagaaaaaag tcgccgagat tcagggccac gcgggaggac
     2221 gccgctcagg gcagagccat gagctcctgc gggacttcga ggcgacactt ctgcagatgt
     2281 ttgggctgcg ccgccgcccg cagcctagca agagtgccgt cattccggac tacatgcggg
     2341 atctttaccg gcttcagtct ggggaggagg aggaagagca gatccacagc actggtcttg
     2401 agtatcctga gcgcccggcc agccgggcca acaccgtgag gagcttccac cacgaagaac
     2461 atctggagaa catcccaggg accagtgaaa actctgcttt tcgtttcctc tttaacctca
     2521 gcagcatccc tgagaacgag gcgatctcct ctgcagagct tcggctcttc cgggagcagg
     2581 tggaccaggg ccctgattgg gaaaggggct tccaccgtat aaacatttat gaggttatga
     2641 agcccccagc agaagtggtg cctgggcacc tcatcacacg actactggac acgagactgg
     2701 tccaccacaa tgtgacacgg tgggaaactt ttgatgtgag ccctgcggtc cttcgctgga
     2761 cccgggagaa gcagccaaac tatgggctag ccattgaggt gactcacctc catcagactc
     2821 ggacccacca gggccagcat gtcaggatta gccgatcgtt acctcaaggg agtgggaatt
     2881 gggcccagct ccggcccctc ctggtcacct ttggccatga tggccggggc catgccttga
     2941 cccgacgccg gagggccaag cgtagcccta agcatcactc acagcgggcc aggaagaaga
     3001 ataagaactg ccggcgccac tcgctctatg tggacttcag cgatgtgggc tggaatgact
     3061 ggattgtggc cccaccaggc taccaggcct tctactgcca tggggactgc ccctttccac
     3121 tggctgacca cctcaactca accaaccatg ccattgtgca gaccctggtc aattctgtca
     3181 attccagtat ccccaaagcc tgttgtgtgc ccactgaact gagtgccatc tccatgctgt
     3241 acctggatga gtatgataag gtggtactga aaaattatca ggagatggta gtagagggat
     3301 gtgggtgccg ctgagcggcc gctagacgcg tcgacaatca acctctggat tacaaaattt
     3361 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3421 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3481 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3541 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3601 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3661 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3721 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     3781 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3841 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3901 tctccctttg ggccgcctcc ccgaagcttg tcgagaagta ctagaggatc ataatcagcc
     3961 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     4021 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     4081 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     4141 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactggcgc
     4201 gccgtgatgc gtatttctga atctttgtaa aatagcacac aagactccaa cgcgtttggc
     4261 gttttatttt cttgctcgac tctagtttat taggccttac tttccaagtc ggttcatctc
     4321 tatgtctgta taaatctgtc ttttcttggt gtgctttaat ttaatgcaaa gatggatacc
     4381 aactcggaga accaagaata gtccaatgat taaccctatg ataaagaaaa aagaggcaat
     4441 agagcttttc caactactga accaaccttc tacaagctcg attggatttt tggatagccc
     4501 agtatcaccc cggggtacct gcagatccgc aaaggcagaa tgcgccgccg ccgccaaaag
     4561 cacatataaa acaatagcgc ttaccatctt gcttgtgtgt tccttattga agccttggtg
     4621 tgactgattt actagtagca tagatccgta tttataggtt tttttattac aaaactgtta
     4681 cgaaaacagt aaaatactta tttatttgcg agatggttat cattttaatt atctccatga
     4741 tccagccaac cactagaact atagctagag tcctgggcga acaaaatggt ggcagcttgt
     4801 gatattagtt tgtgcgtctc attacaatgg ctgttatttt taacaacaaa caactgctcg
     4861 cagacaatag tatagaaaag ggaggtgaac tgtttttgtt taacggttcg tacaacattt
     4921 tggaaagtta tgttaatccg gtgctgctaa aaaatggtgt aattgaacta gaagaagctg
     4981 cgtactatgc cggcaacata ttgtacaaaa ccgacgatcc caaattcatt gattatataa
     5041 atttaataat taaagcaaca cactccgaag aactaccaga aaatagcact gttgtaaatt
     5101 acagaaaaac tatgcgcagc ggtactatac accccattaa aaaagacata tatatttatg
     5161 acaacaaaaa atttactcta tacgatagat acatatatgg atacgataat aactatgtta
     5221 atttttatga ggagaaaaat gaaaaagaga aggaatacga agaagaagac gacaaggcgt
     5281 ctagtttatg tgaaaataaa attatattgt cgcaaattaa ctgtgaatca tttgaaaatg
     5341 attttaaata ttacctcagc gattataact acgcgttttc aattatagat aatactacaa
     5401 atgttcttgt tgcgtttggt ttgtatcgtt aataaaaaac aaatttagca tttataattg
     5461 ttttattatt caataattac aaataggatt gagacccttg cagttgccag caaacggaca
     5521 gagcttgtcc acagttttca ttttttggca gtttggtggt ttatacacat cttgggattt
     5581 gattaaaaga tgcatgactt cacccacaca cttggagtcg tggttgtaga aatgcgtcag
     5641 agccttgatt aaacatcggt tttcacactt ggcacacgaa accacgtttg caatgtaaca
     5701 gtttagtata aacctcttcc tgtacatacc gccgcgcggt acattgtttt ctttcaaatc
     5761 gttcaatatt ttttttatac acggaggcat attaatcttg ttgttcagca tgtccacgac
     5821 atttttcttt ccttctacgc attctttcat acaaatgttt ctcttgctgc gttttttaaa
     5881 attggaattt ttaatttcat taatagtggg cggagccgtt aacagcatgt acaatcgcag
     5941 tccggaaatt ttgaccaaaa ccccgccatt gtagaatacc gtgtatggag ttagggtcaa
     6001 tttatcaata aaatcatcgg gatcaattaa atatgtagct ttcttgtcta aacatgacgc
     6061 tctaatgagc ggactccaca cgttatacga tgcattcgcc atacttcgcg gcttctcgca
     6121 ccagcggtcc tgattcgact aacggtcgat tggatggctt aatagtttta acgtcacggc
     6181 cttgacggcc ttccgccaat tcgccctata gtgagtcgta ttacgtcgcg ctcactggcc
     6241 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     6301 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga aacgcccttc
     6361 ccaacagttg cgcagcctga atggcgaatg ggagcgccct gtagcggcca ctcaacccta
     6421 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6481 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6541 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6601 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6661 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6721 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6781 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6841 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6901 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6961 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7021 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7081 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     7141 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     7201 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7261 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7321 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7381 gcgtggttct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7441 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7501 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7561 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7621 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7681 agaaaagatc a
//
