LOCUS       sequence-209031-        7655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, hShh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Wagner I, Wang H, Weissert PM, Straube WL, Shevchenko A, Gentzel M, 
            Brito G, Tazaki A, Oliveira C, Sugiura T, Shevchenko A, Simon A, 
            Drechsel DN, Tanaka EM
  TITLE     Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of 
            Dedifferentiating Muscle Cells during Newt Limb Regeneration.
  JOURNAL   Dev Cell. 2017 Mar 27;40(6):608-617.e6. doi: 
            10.1016/j.devcel.2017.03.002.
  PUBMED    28350991
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.03"; journalName: "Dev Cell"; date: 
            "2017-03-27- 27"; volume: "40"; issue: "6"; pages: "608-617.e6"
COMMENT     Sequence Label: pOEM1_pCMV:deltaN-bmp4-pPH:vsvged
FEATURES             Location/Qualifiers
     source          1..7655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     692..709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           1061..1433
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        1534..1837
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1838..2041
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1992..2012
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(3353..3373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3771..3782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4017..4151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4054..4073)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4108..4127
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(4264..4296)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(4492..4551)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(4614..4705)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4672..4689)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4791..5396
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(5434..5558)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(6168..6187)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6169..6187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6198..6215)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6198..6214)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6207..6229)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        6530..6634
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6635..6703,6704..7495)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6853..6872)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcattagg
      661 caccccaggc tttacccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggagag
      721 cgcccaatac gcaaggaaac agctatgacc atgttaatgc agctggcacg acaggtttcc
      781 cgactggaaa gcgggcagtg agcggaaggc ccatgaggcc caaaaagctc aactacaaat
      841 ttgatcgcgg acgtgttagc cgacacaatt aataggcgtc gtgtggctat ggcaaaatcg
      901 tcttcggaag caacttctaa cgacgagggt tgggacgacg acgataatcg gcctaataaa
      961 gctaacacgc ccgatgttaa atatgtccaa gcgttattta acgtgtttac gtcgagtcaa
     1021 ttgtacacta acgacagtga tgaaagaaat acaaaagcgc ataatatttt gaacgacgtc
     1081 gaacctttat tacaaaacaa aacacaaacg aatatcgaca aagctagatt gctgctacaa
     1141 gatttggcaa gttttgtggc gttgagcgaa aatccattag atagtccagc catcggttcg
     1201 gaaaaacaac ccttgtttga aactaatcga aacctatttt acaaatctat tgaggattta
     1261 atatttaaat tcagatataa agacgctgaa aatcatttga ttttcgctct aacataccac
     1321 cctaaagatt ataaatttaa tgaattatta aaatacgtac aacaattgtc tgtaaatcaa
     1381 caacgcacag aatctagcgc ttaataaatg tactaataac aatgtatcgt gttttattaa
     1441 ttaagatctg cggatgcagg ctccgccatg cattagttat taatagtaat caattacggg
     1501 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1561 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1621 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1681 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1741 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1801 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     1861 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1921 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     1981 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2041 tggtttagtg aaccgtcaga tccgctagcg ctaccggtgc cgccaccatg attcctggta
     2101 accgaatgct gatggtcgtt ttattatgcc aagtcctgct aggaggcgcg agccatgcta
     2161 gtttgatacc tgagacgggg aagaaaaaag tcgccgagat tcagggccac gcgggaggac
     2221 gccgctcagg gcagagccat gagctcctgc gggacttcga ggcgacactt ctgcagatgt
     2281 ttgggctgcg ccgccgcccg cagcctagca agagtgccgt cattccggac tacatgcggg
     2341 atctttaccg gcttcagtct ggggaggagg aggaagagca gatccacagc actggtcttg
     2401 agtatcctga gcgcccggcc agccgggcca acaccgtgag gagcttccac cacgaagaac
     2461 atctggagaa catcccaggg accagtgaaa actctgcttt tcgtttcctc tttaacctca
     2521 gcagcatccc tgagaacgag gcgatctcct ctgcagagct tcggctcttc cgggagcagg
     2581 tggaccaggg ccctgattgg gaaaggggct tccaccgtat aaacatttat gaggttatga
     2641 agcccccagc agaagtggtg cctgggcacc tcatcacacg actactggac acgagactgg
     2701 tccaccacaa tgtgacacgg tgggaaactt ttgatgtgag ccctgcggtc cttcgctgga
     2761 cccgggagaa gcagccaaac tatgggctag ccattgaggt gactcacctc catcagactc
     2821 ggacccacca gggccagcat gtcaggatta gccgatcgtt acctcaaggg agtgggaatt
     2881 gggcccagct ccggcccctc ctggtcacct ttggccatga tggccggggc catgccttga
     2941 cccgacgccg gagggccaag cgtagcccta actgccggcg ccactcgctc tatgtggact
     3001 tcagcgatgt gggctggaat gactggattg tggccccacc aggctaccag gccttctact
     3061 gccatgggga ctgccccttt ccactggctg accacctcaa ctcaaccaac catgccattg
     3121 tgcagaccct ggtcaattct gtcaattcca gtatccccaa agcctgttgt gtgcccactg
     3181 aactgagtgc catctccatg ctgtacctgg atgagtatga taaggtggta ctgaaaaatt
     3241 atcaggagat ggtagtagag ggatgtgggt gccgctgagc ggccgctaga cgcgtcgaca
     3301 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3361 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3421 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3481 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3541 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3601 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3661 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     3721 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3781 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3841 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgaag cttgtcgaga
     3901 agtactagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     3961 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4021 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4081 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4141 atgtctggat ctgatcactg gcgcgccgtg atgcgtattt ctgaatcttt gtaaaatagc
     4201 acacaagact ccaacgcgtt tggcgtttta ttttcttgct cgactctagt ttattaggcc
     4261 ttactttcca agtcggttca tctctatgtc tgtataaatc tgtcttttct tggtgtgctt
     4321 taatttaatg caaagatgga taccaactcg gagaaccaag aatagtccaa tgattaaccc
     4381 tatgataaag aaaaaagagg caatagagct tttccaacta ctgaaccaac cttctacaag
     4441 ctcgattgga tttttggata gcccagtatc accccggggt acctgcagat ccgcaaaggc
     4501 agaatgcgcc gccgccgcca aaagcacata taaaacaata gcgcttacca tcttgcttgt
     4561 gtgttcctta ttgaagcctt ggtgtgactg atttactagt agcatagatc cgtatttata
     4621 ggttttttta ttacaaaact gttacgaaaa cagtaaaata cttatttatt tgcgagatgg
     4681 ttatcatttt aattatctcc atgatccagc caaccactag aactatagct agagtcctgg
     4741 gcgaacaaaa tggtggcagc ttgtgatatt agtttgtgcg tctcattaca atggctgtta
     4801 tttttaacaa caaacaactg ctcgcagaca atagtataga aaagggaggt gaactgtttt
     4861 tgtttaacgg ttcgtacaac attttggaaa gttatgttaa tccggtgctg ctaaaaaatg
     4921 gtgtaattga actagaagaa gctgcgtact atgccggcaa catattgtac aaaaccgacg
     4981 atcccaaatt cattgattat ataaatttaa taattaaagc aacacactcc gaagaactac
     5041 cagaaaatag cactgttgta aattacagaa aaactatgcg cagcggtact atacacccca
     5101 ttaaaaaaga catatatatt tatgacaaca aaaaatttac tctatacgat agatacatat
     5161 atggatacga taataactat gttaattttt atgaggagaa aaatgaaaaa gagaaggaat
     5221 acgaagaaga agacgacaag gcgtctagtt tatgtgaaaa taaaattata ttgtcgcaaa
     5281 ttaactgtga atcatttgaa aatgatttta aatattacct cagcgattat aactacgcgt
     5341 tttcaattat agataatact acaaatgttc ttgttgcgtt tggtttgtat cgttaataaa
     5401 aaacaaattt agcatttata attgttttat tattcaataa ttacaaatag gattgagacc
     5461 cttgcagttg ccagcaaacg gacagagctt gtccacagtt ttcatttttt ggcagtttgg
     5521 tggtttatac acatcttggg atttgattaa aagatgcatg acttcaccca cacacttgga
     5581 gtcgtggttg tagaaatgcg tcagagcctt gattaaacat cggttttcac acttggcaca
     5641 cgaaaccacg tttgcaatgt aacagtttag tataaacctc ttcctgtaca taccgccgcg
     5701 cggtacattg ttttctttca aatcgttcaa tatttttttt atacacggag gcatattaat
     5761 cttgttgttc agcatgtcca cgacattttt ctttccttct acgcattctt tcatacaaat
     5821 gtttctcttg ctgcgttttt taaaattgga atttttaatt tcattaatag tgggcggagc
     5881 cgttaacagc atgtacaatc gcagtccgga aattttgacc aaaaccccgc cattgtagaa
     5941 taccgtgtat ggagttaggg tcaatttatc aataaaatca tcgggatcaa ttaaatatgt
     6001 agctttcttg tctaaacatg acgctctaat gagcggactc cacacgttat acgatgcatt
     6061 cgccatactt cgcggcttct cgcaccagcg gtcctgattc gactaacggt cgattggatg
     6121 gcttaatagt tttaacgtca cggccttgac ggccttccgc caattcgccc tatagtgagt
     6181 cgtattacgt cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6241 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6301 gaggcccgca ccgaaacgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggagcg
     6361 ccctgtagcg gccactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6421 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     6481 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6541 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6601 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6661 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6721 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6781 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6841 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6901 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6961 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7021 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7081 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7141 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7201 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7261 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7321 tgctgataaa tctggagccg gtgagcgtgg ttctcgcggt atcattgcag cactggggcc
     7381 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7441 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7501 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7621 gttccactga gcgtcagacc ccgtagaaaa gatca
//
