LOCUS       sequence-209032-        7101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus rescue vector, VSVGED pseudotype, hShh.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Nacu E, Gromberg E, Oliveira CR, Drechsel D, Tanaka EM
  TITLE     FGF8 and SHH substitute for anterior-posterior tissue interactions 
            to induce limb regeneration.
  JOURNAL   Nature. 2016 May 19;533(7603):407-10. doi: 10.1038/nature17972.
  PUBMED    27120163
REFERENCE   2  (bases 1 to 7101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-05-19- 19"; volume: "533"; issue: "7603"; 
            pages: "407-10"
COMMENT     Sequence Label: pOEM1_pCMV:axFgf8-pPH:vsvged
FEATURES             Location/Qualifiers
     source          1..7101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     500..519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     692..709
                     /label=L4440
                     /note="L4440 vector, forward primer"
     3'UTR           1061..1433
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        1534..1837
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1838..2041
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1992..2012
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2799..2819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3217..3228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3463..3597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3500..3519)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3554..3573
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3710..3742)
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     sig_peptide     complement(3938..3997)
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     promoter        complement(4060..4151)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(4118..4135)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4237..4842
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(4880..5004)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(5614..5633)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5615..5633)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5644..5661)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5644..5660)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5653..5675)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        5976..6080
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6081..6149,6150..6941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6299..6318)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
       61 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
      421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcattagg
      661 caccccaggc tttacccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggagag
      721 cgcccaatac gcaaggaaac agctatgacc atgttaatgc agctggcacg acaggtttcc
      781 cgactggaaa gcgggcagtg agcggaaggc ccatgaggcc caaaaagctc aactacaaat
      841 ttgatcgcgg acgtgttagc cgacacaatt aataggcgtc gtgtggctat ggcaaaatcg
      901 tcttcggaag caacttctaa cgacgagggt tgggacgacg acgataatcg gcctaataaa
      961 gctaacacgc ccgatgttaa atatgtccaa gcgttattta acgtgtttac gtcgagtcaa
     1021 ttgtacacta acgacagtga tgaaagaaat acaaaagcgc ataatatttt gaacgacgtc
     1081 gaacctttat tacaaaacaa aacacaaacg aatatcgaca aagctagatt gctgctacaa
     1141 gatttggcaa gttttgtggc gttgagcgaa aatccattag atagtccagc catcggttcg
     1201 gaaaaacaac ccttgtttga aactaatcga aacctatttt acaaatctat tgaggattta
     1261 atatttaaat tcagatataa agacgctgaa aatcatttga ttttcgctct aacataccac
     1321 cctaaagatt ataaatttaa tgaattatta aaatacgtac aacaattgtc tgtaaatcaa
     1381 caacgcacag aatctagcgc ttaataaatg tactaataac aatgtatcgt gttttattaa
     1441 ttaagatctg cggatgcagg ctccgccatg cattagttat taatagtaat caattacggg
     1501 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     1561 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     1621 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     1681 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     1741 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     1801 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     1861 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     1921 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     1981 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     2041 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatgaa caacgcgagc
     2101 tcgtcagtcg tcggatatct attgctgcat ctttttgtcc tctgcatgca agcaaaggta
     2161 actgttcagt ccccacctaa ttttacacag catgtgaggg agcagagcct ggtgacagac
     2221 cagctgagcc ggcggctcat ccgcacctac cagctgtaca gccgcaccag tgggaagcac
     2281 gtgcaggtcc ttggcaacaa gaagatcaac gccatggccg acgacggcga ctcgcacgcc
     2341 aaattaattg tggaaacgga caccttcgga agccgcgtcc gcattaaagg agccgagacg
     2401 ggctattaca tctgcatgaa caagaaggga aagctgattg gcaagagcaa tggccgaggc
     2461 aaagactgcg tcttctccga gatagtgctc gagaacaact acacggcact acaaaacgcc
     2521 aaatacgaag gatggtacat ggccttcaca aggaaaggcc gcccgaggaa aggctccaag
     2581 accaggcagc accagcggga ggtccacttc atgaagcgac tgccgaaagg ccaccaaaca
     2641 acggagcctc atagacgctt cgagtttgta aattatcctt tcaacaggcg gagcaaaaga
     2701 actcgacatt ccgtaccgcg ataggcggcc gctagacgcg tcgacaatca acctctggat
     2761 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2821 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2881 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2941 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3001 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3061 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3121 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     3181 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3241 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3301 acgagtcgga tctccctttg ggccgcctcc ccgaagcttg tcgagaagta ctagaggatc
     3361 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     3421 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     3481 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3541 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga
     3601 tcactggcgc gccgtgatgc gtatttctga atctttgtaa aatagcacac aagactccaa
     3661 cgcgtttggc gttttatttt cttgctcgac tctagtttat taggccttac tttccaagtc
     3721 ggttcatctc tatgtctgta taaatctgtc ttttcttggt gtgctttaat ttaatgcaaa
     3781 gatggatacc aactcggaga accaagaata gtccaatgat taaccctatg ataaagaaaa
     3841 aagaggcaat agagcttttc caactactga accaaccttc tacaagctcg attggatttt
     3901 tggatagccc agtatcaccc cggggtacct gcagatccgc aaaggcagaa tgcgccgccg
     3961 ccgccaaaag cacatataaa acaatagcgc ttaccatctt gcttgtgtgt tccttattga
     4021 agccttggtg tgactgattt actagtagca tagatccgta tttataggtt tttttattac
     4081 aaaactgtta cgaaaacagt aaaatactta tttatttgcg agatggttat cattttaatt
     4141 atctccatga tccagccaac cactagaact atagctagag tcctgggcga acaaaatggt
     4201 ggcagcttgt gatattagtt tgtgcgtctc attacaatgg ctgttatttt taacaacaaa
     4261 caactgctcg cagacaatag tatagaaaag ggaggtgaac tgtttttgtt taacggttcg
     4321 tacaacattt tggaaagtta tgttaatccg gtgctgctaa aaaatggtgt aattgaacta
     4381 gaagaagctg cgtactatgc cggcaacata ttgtacaaaa ccgacgatcc caaattcatt
     4441 gattatataa atttaataat taaagcaaca cactccgaag aactaccaga aaatagcact
     4501 gttgtaaatt acagaaaaac tatgcgcagc ggtactatac accccattaa aaaagacata
     4561 tatatttatg acaacaaaaa atttactcta tacgatagat acatatatgg atacgataat
     4621 aactatgtta atttttatga ggagaaaaat gaaaaagaga aggaatacga agaagaagac
     4681 gacaaggcgt ctagtttatg tgaaaataaa attatattgt cgcaaattaa ctgtgaatca
     4741 tttgaaaatg attttaaata ttacctcagc gattataact acgcgttttc aattatagat
     4801 aatactacaa atgttcttgt tgcgtttggt ttgtatcgtt aataaaaaac aaatttagca
     4861 tttataattg ttttattatt caataattac aaataggatt gagacccttg cagttgccag
     4921 caaacggaca gagcttgtcc acagttttca ttttttggca gtttggtggt ttatacacat
     4981 cttgggattt gattaaaaga tgcatgactt cacccacaca cttggagtcg tggttgtaga
     5041 aatgcgtcag agccttgatt aaacatcggt tttcacactt ggcacacgaa accacgtttg
     5101 caatgtaaca gtttagtata aacctcttcc tgtacatacc gccgcgcggt acattgtttt
     5161 ctttcaaatc gttcaatatt ttttttatac acggaggcat attaatcttg ttgttcagca
     5221 tgtccacgac atttttcttt ccttctacgc attctttcat acaaatgttt ctcttgctgc
     5281 gttttttaaa attggaattt ttaatttcat taatagtggg cggagccgtt aacagcatgt
     5341 acaatcgcag tccggaaatt ttgaccaaaa ccccgccatt gtagaatacc gtgtatggag
     5401 ttagggtcaa tttatcaata aaatcatcgg gatcaattaa atatgtagct ttcttgtcta
     5461 aacatgacgc tctaatgagc ggactccaca cgttatacga tgcattcgcc atacttcgcg
     5521 gcttctcgca ccagcggtcc tgattcgact aacggtcgat tggatggctt aatagtttta
     5581 acgtcacggc cttgacggcc ttccgccaat tcgccctata gtgagtcgta ttacgtcgcg
     5641 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5701 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5761 aacgcccttc ccaacagttg cgcagcctga atggcgaatg ggagcgccct gtagcggcca
     5821 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5881 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5941 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6001 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6061 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6121 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6181 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6241 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6301 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6361 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6421 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6481 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6541 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6601 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6661 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6721 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6781 gagccggtga gcgtggttct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6841 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6901 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6961 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7021 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7081 cagaccccgt agaaaagatc a
//
