LOCUS       sequence-209038-        1371 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human gamma-actin (codon is optimised for expression in 
            Pichia pastoris) fused with thymosin beta and a His tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Hatano T, Alioto S, Roscioli E, Palani S, Clarke ST, Kamnev A, 
            Hernandez-Fernaud JR, Sivashanmugam L, Chapa-Y-Lazo B, Jones AME, 
            Robinson RC, Sampath K, Mishima M, McAinsh AD, Goode BL, 
            Balasubramanian MK
  TITLE     Rapid production of pure recombinant actin isoforms in Pichia 
            pastoris.
  JOURNAL   J Cell Sci. 2018 Mar 13. pii: jcs.213827. doi: 10.1242/jcs.213827.
  PUBMED    29535210
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2018 Mar 13. pii: jcs.213827. doi: 10.1242/jcs.213827."
COMMENT     Sequence Label: pPICZc-HsACTG1*-thymosinB-8His
FEATURES             Location/Qualifiers
     source          1..1371
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1315..1335
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1339..1362
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gaattcgcca ccatggaaga agagatcgcc gctttggtta tcgacaacgg ttctggtatg
       61 tgtaaggctg gtttcgctgg tgatgatgct ccaagagctg ttttcccatc catcgttggt
      121 agaccaagac accagggtgt tatggttggt atgggtcaaa aggactccta cgttggtgac
      181 gaagctcaat ccaagagagg tatcttgacc ctgaagtacc caatcgagca cggtattgtc
      241 actaactggg acgacatgga aaagatctgg caccacactt tctacaacga gttgagagtt
      301 gctccagagg aacacccagt tttgttgact gaagctccat tgaacccaaa ggccaacaga
      361 gaaaagatga cccagatcat gttcgagact ttcaacaccc cagctatgta cgttgctatc
      421 caggctgttt tgtccttgta cgcttccggt agaactaccg gtatcgttat ggattctggt
      481 gacggtgtta ctcacaccgt tccaatctac gaaggttacg ctttgccaca cgccatcttg
      541 agattggatt tggctggtag agacttgacc gactacctga tgaagatctt gaccgagaga
      601 ggttactcct tcactactac tgctgagaga gagatcgtca gggacatcaa agaaaagttg
      661 tgctacgtcg ccctggactt cgaacaagaa atggctactg ctgcttcctc atcctctttg
      721 gaaaagtcct acgaattgcc agacggtcag gttatcacta tcggtaacga gagattcaga
      781 tgcccagagg ctttgttcca accatccttc cttggtatgg aatcctgtgg tatccacgag
      841 actaccttca actccatcat gaagtgtgac gtcgacatca gaaaggactt gtacgccaac
      901 accgttttgt ccggtggtac tactatgtac ccaggtatcg ctgacagaat gcagaaagag
      961 attactgctt tggccccatc caccatgaag atcaagatta ttgctccacc agagaggaag
     1021 tactccgttt ggattggtgg ttctatcttg gcttccttgt ccaccttcca gcagatgtgg
     1081 atttccaagc aagaatacga cgaatccggt ccttccatcg ttcacagaaa gtgttttgct
     1141 agccgtggcg gcagcggtgg gagctctggc ggtagcgctt ccgataagcc agatatggcg
     1201 gaaatcgaaa agttcgataa atctaaactg aagaaaactg agactcaaga aaagaacccg
     1261 ctgccgagca aggaaaccat cgagcaggag aagcaggcgg gtgaatccgg taccgaaaat
     1321 ttatattttc aagggcccca ccaccaccac caccaccacc acgaagaata a
//
