LOCUS       sequence-209039-        1371 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Saccharomyces cerevisiae Act1 (codon is optimised for 
            expression in Pichia pastoris) fused with thymosin beta and a His 
            tag. TH3-37.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Hatano T, Alioto S, Roscioli E, Palani S, Clarke ST, Kamnev A, 
            Hernandez-Fernaud JR, Sivashanmugam L, Chapa-Y-Lazo B, Jones AME, 
            Robinson RC, Sampath K, Mishima M, McAinsh AD, Goode BL, 
            Balasubramanian MK
  TITLE     Rapid production of pure recombinant actin isoforms in Pichia 
            pastoris.
  JOURNAL   J Cell Sci. 2018 Mar 13. pii: jcs.213827. doi: 10.1242/jcs.213827.
  PUBMED    29535210
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2018 Mar 13. pii: jcs.213827. doi: 10.1242/jcs.213827."
COMMENT     Sequence Label: pPICZc-ScAct1*-thymosinB-8His
FEATURES             Location/Qualifiers
     source          1..1371
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1315..1335
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1339..1362
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gaattcgcca ccatggactc tgaggttgct gctttggtta tcgacaacgg ttccggtatg
       61 tgtaaggctg gttttgctgg tgatgatgct ccaagagctg ttttcccatc catcgttggt
      121 agaccaagac accagggtat catggttggt atgggtcaaa aggactccta cgttggtgac
      181 gaagctcaat ccaagagagg tatcttgacc ctgagatacc caatcgagca cggtattgtc
      241 actaactggg acgacatgga aaagatctgg caccacactt tctacaacga gttgagagtt
      301 gctccagagg aacacccagt tttgttgact gaagctccaa tgaacccaaa gtccaaccgt
      361 gagaagatga cccagatcat gttcgagact ttcaacgtcc cagccttcta cgtttccatc
      421 caagctgttt tgtccctgta ctcctccggt agaactaccg gtatcgtttt ggattctggt
      481 gacggtgtta cccacgttgt tccaatctac gctggtttct ctttgccaca cgccatcttg
      541 agaattgact tggctggtag agacttgacc gactacttga tgaagatcct gtccgagaga
      601 ggttactcct tctccactac tgctgaaaga gagatcgtca gggacatcaa agaaaagttg
      661 tgctacgtcg ccctggactt cgaacaagaa atgcaaactg ctgctcagtc cagctccatt
      721 gaaaagtctt acgaattgcc agacggtcag gtcatcacta ttggtaacga aagattcaga
      781 gccccagagg ccttgtttca cccatctgtt ttgggtcttg agtccgctgg tatcgaccag
      841 actacttaca actccatcat gaagtgcgac gtggacgtca gaaaagagct gtacggtaac
      901 atcgttatgt ccggtggtac tactatgttc ccaggtatcg ctgagagaat gcagaaagag
      961 atcactgctt tggctccatc ctccatgaag gtcaagatta ttgctccacc agagagaaag
     1021 tactccgtct ggattggtgg ttctatcttg gcttccttga ctaccttcca gcagatgtgg
     1081 atttccaagc aagaatacga cgaatccggt ccttctatcg ttcaccacaa gtgttttgct
     1141 agccgtggcg gcagcggtgg gagctctggc ggtagcgctt ccgataagcc agatatggcg
     1201 gaaatcgaaa agttcgataa atctaaactg aagaaaactg agactcaaga aaagaacccg
     1261 ctgccgagca aggaaaccat cgagcaggag aagcaggcgg gtgaatccgg taccgaaaat
     1321 ttatattttc aagggcccca ccaccaccac caccaccacc acgaagaata a
//
