LOCUS       sequence-209041-        1623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses arginylated human beta-actin fused with thymosin beta and 
            a His tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Hatano T, Alioto S, Roscioli E, Palani S, Clarke ST, Kamnev A, 
            Hernandez-Fernaud JR, Sivashanmugam L, Chapa-Y-Lazo B, Jones AME, 
            Robinson RC, Sampath K, Mishima M, McAinsh AD, Goode BL, 
            Balasubramanian MK
  TITLE     Rapid production of pure recombinant actin isoforms in Pichia 
            pastoris.
  JOURNAL   J Cell Sci. 2018 Mar 13. pii: jcs.213827. doi: 10.1242/jcs.213827.
  PUBMED    29535210
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2018 Mar 13. pii: jcs.213827. doi: 10.1242/jcs.213827."
COMMENT     Sequence Label: pPICZc-Ub-R-HsACTB*-thymosinB-8His
FEATURES             Location/Qualifiers
     source          1..1623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..267
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             1567..1587
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1591..1614
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 aaaaaacaac taattattcg aaacgaggaa ttcgccacca tgcagatttt cgtcaagact
       61 ttgaccggta aaaccataac attggaagtt gaatcttccg ataccatcga caacgttaag
      121 tcgaaaattc aagacaagga aggtatccct ccagatcaac aaagattgat ctttgccggt
      181 aagcagctag aagacggtag aacgctgtct gattacaaca ttcagaagga gtccacctta
      241 catcttgtgc taaggctaag aggtggtaga gatgatatcg ccgcgctcgt cgtcgacaac
      301 ggctccggca tgtgcaaggc cggcttcgcg ggcgacgatg ccccccgggc cgtcttcccc
      361 tccatcgtgg ggcgccccag gcaccagggc gtgatggtgg gcatgggtca gaaggattcc
      421 tatgtgggcg acgaggccca gagcaagaga ggcatcctca ccctgaagta ccccatcgag
      481 cacggcatcg tcaccaactg ggacgacatg gagaaaatct ggcaccacac cttctacaat
      541 gagctgcgtg tggctcccga ggagcacccc gtgctgctga ccgaggcccc cctgaacccc
      601 aaggccaacc gcgagaagat gacccagatc atgtttgaga ccttcaacac cccagccatg
      661 tacgttgcta tccaggctgt gctatccctg tacgcctctg gccgtaccac tggcatcgtg
      721 atggactccg gtgacggggt cacccacact gtgcccatct acgaggggta tgccctcccc
      781 catgccatcc tgcgtctgga cctggctggc cgggacctga ctgactacct catgaagatc
      841 ctcaccgagc gcggctacag cttcaccacc acggccgagc gggaaatcgt gcgtgacatt
      901 aaggagaagc tgtgctacgt cgccctggac ttcgagcaag agatggccac ggctgcttcc
      961 agctcctccc tggagaagag ctacgagctg cctgacggcc aggtcatcac cattggcaat
     1021 gagcggttcc gctgccctga ggcactcttc cagccttcct tcctgggcat ggagtcctgt
     1081 ggcatccacg aaactacctt caactccatc atgaagtgtg acgtggacat ccgcaaagac
     1141 ctgtacgcca acacagtgct gtctggcggc accaccatgt accctggcat tgccgacagg
     1201 atgcagaagg agatcactgc cctggcaccc agcacaatga agatcaagat cattgctcct
     1261 cctgagcgca agtactccgt gtggatcggc ggctccatcc tggcctcgct gtccaccttc
     1321 cagcagatgt ggatcagcaa gcaggagtat gacgagtccg gcccctccat cgtccaccgc
     1381 aaatgcttcg ctagccgtgg cggcagcggt gggagctctg gcggtagcgc ttccgataag
     1441 ccagatatgg cggaaatcga aaagttcgat aaatctaaac tgaagaaaac tgagactcaa
     1501 gaaaagaacc cgctgccgag caaggaaacc atcgagcagg agaagcaggc gggtgaatcc
     1561 ggtaccgaaa atttatattt tcaagggccc caccaccacc accaccacca ccacgaagaa
     1621 taa
//
