LOCUS       sequence-209042-       10672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  scGESTALT V7 lineage barcode for zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Raj B, Wagner DE, McKenna A, Pandey S, Klein AM, Shendure J, Gagnon 
            JA, Schier AF
  TITLE     Simultaneous single-cell profiling of lineages and cell types in the
            vertebrate brain.
  JOURNAL   Nat Biotechnol. 2018 Mar 28. pii: nbt.4103. doi: 10.1038/nbt.4103.
  PUBMED    29608178
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Mar 28. pii: nbt.4103. doi: 10.1038/nbt.4103."
COMMENT     Sequence Label: pTol2-hspDRv7_scGstlt
FEATURES             Location/Qualifiers
     source          1..10672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2499..2510,2511..2673)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      4689..4698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4695..5372
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(4825..4843)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4875..4895)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5093..5112
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5287..5310
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    5748..5882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(6184..6897,6898..6900,6901..6903))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6187..6897,6898..6900,6901..6903))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6229..6250)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6578..6597
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6837..6858
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(8202..8389,8390..8401)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(8435..8451)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8435..8451)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8488..8508)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8488..8506)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8527..8543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8527..8543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8540..8562)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8551..8567
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8575..8581,8582..8599,8600..8605))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8620..8641
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8758..8775)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8929..9517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9009..9028)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9688..10479,10480..10548))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10311..10330
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10549..10653)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggtaga ttgattgatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattggtagt caaaataaga aataatttcc acagattcat tacagaaatg attaaatgca
     1561 tacataaaaa actggggggg gggggataca acaacacact taagtcacat ttgcctacgt
     1621 aaagaaaagt aaagaaaatc aatagctata ttttacatct cctttttttg ctgtctttta
     1681 attagccttg ttttgctgtt atcttgattg aaactgtaac ttcttcacct gcttcttttc
     1741 tttgtaggtt ttgccagccc agaaacaggc atggctgtgc aaggccagag caccatgcac
     1801 aatgcgctgc atgtcttcat gaacggctca atgtcctcag tccagggctc agccaacgac
     1861 cccatcttcc tccttcacca tgctttcatt gacaggtaac aaacacgtca tgacattaga
     1921 ctgcacagtt tttgacaaag ttcatacaat ctgttgttta tagctgctac aattagtgaa
     1981 gtttgtgaat gtacttggat gagcagcgaa agatcaattg agatcaattg ttagagtttg
     2041 gttgccctgc agagcaaaga acaaaaaata atctggtggc tttactgcgt gaggttatta
     2101 ttggtggaat agaaacacaa aacataattg catttatttg tttaattttt tatcttatct
     2161 taactttcat cttgcatatt tgtttcttac atcatttcta gcatctttga gcgctggcta
     2221 agaactcatc agcctccccg gtccatctac ccacgtacca atgcaccaat tggccacaat
     2281 gacggctact acatggtgcc attccttcct ctttatagga atggagacta cctcctgtcc
     2341 aacaatgctc ttggatacga gtacgcctac ctgttggacc caggtcattg cacaacacca
     2401 gaaatgccct ctgatctgca aaagacgtga ataatctgtt cagacaccca tatgcactct
     2461 gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa
     2521 aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa
     2581 agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact
     2641 tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct
     2701 tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt
     2761 ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag
     2821 ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc
     2881 ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc
     2941 gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc
     3001 agagactccc tggtgtctga aacacaggcc agatgggcca ttcgtacgct taatttcgta
     3061 tagggataac agggtaatga tctaggtcga caccggtgat atcgggcccc ctaggatcga
     3121 tgaattcgat atctcagggg tgtcgcttgg ttatttccaa aaatcaaatt aattttatta
     3181 aactattaga acgagcatgt tttgtctata tgctacagaa gataaaaaat aataggagtt
     3241 aacagttata aaacaacaca ctttgtttct attgattgtt gaccacactg gggtctcatt
     3301 aagttagatt aaagacacac taactgggtc aaaagcagca gattgatttc atagcaccag
     3361 ggtaaacttt ctaacacttt tacggcaatc atatacatta aaattaaata cagaccacga
     3421 ctgaacaagg aggatgatct ccaatattaa acaaagagac ttgtgcctat ttctctgagg
     3481 gtaaacatga cctctcaagt tagcaagttg tttttaacac tacaaaaata gttaagactg
     3541 caatcccaga ataaagtatt ggttttaacc aatcaatata gtacagtaaa catccatttg
     3601 ttttgttgaa acgttaaaca aatctgacca aagctattag cttatataaa acaggtttgc
     3661 cttctatgta gctgaaaaca ccacaggccc gattttgcta ctgtgtaaaa catttcagca
     3721 agattttttt attgcatttt tttttactga atcgttcaaa cattttatca ttttagtttg
     3781 ttcattcatt gcaactggaa aaacaacaca tcacacaacc gcacatattt cagcaataag
     3841 tacaataaaa cactcaaata aaaaaaccat tttaaatctc tttgtatttt tgaccgctgt
     3901 ttcgcgtaat ttcacggtaa aactctggaa atctccacta cattcctctc agcggctcct
     3961 ctcaatgaca gctgaagaag tgacgcggct gcctgctgtg ttttgattgg tcgaattcac
     4021 tggaggcttc cagaacagtg tagagtctga acgggtgcgc gctctgctgt atttaaaggg
     4081 cgaaagagag accgcagaga aactcaaccg aagagaagcg acttgacaaa gaagaaaaga
     4141 gcagcctgac aggacttttt ccccgacgag gtgtttattc gctctattta agaatctact
     4201 gtaaggtaag tctcaatata ttgtactcta ttggctaatc agaattatat agagattata
     4261 tgtacttaat gtcaaaaaat caactttgta tatgtaatct ttttacatgt ggactgccta
     4321 tgttcatctt attttaggtc tactagaaaa ttatatttcc cgttttcaca ataaggattt
     4381 ttaaaaaaag caatgaacag acgggcattt actttatgtt gctgacatta ttttatatga
     4441 gcataataac cataaatact agcaaatgtc ctaaatgaat ttgtgttaat gttgtctaca
     4501 aaagaaaatt agcgttttac ttgtacaact aataataact tggttattaa gagaatttca
     4561 cttgttgact agaaaaatcc tttcataatg aaacaattgc accataaatt gtataaatat
     4621 aaaattaatt ctaattgttt ttttttttcc tgctgcagcc catcggatcc aaactcgaga
     4681 aagctagcgc caccatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg
     4741 tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     4801 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     4861 tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac gtgaagcacc
     4921 ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag tgggagcgcg
     4981 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     5041 gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt cccctccgac ggccccgtaa
     5101 tgcagaagaa gactatgggc tgggagccct ccaccgagcg cctgtacccc cgcgacggcg
     5161 tgctgaaggg cgagatccac aaggccctga agctgaagga cggcggccac tacctggtgg
     5221 agttcaagtc catctacatg gccaagaagc ccgtgcagct gcccggctac tactacgtgg
     5281 actccaagct ggacatcacc tcccacaacg aggactacac catcgtggag cagtacgagc
     5341 gcgccgaggg ccgccaccac ctgttcctgt agaaatccgg actcagatct cgagctcaag
     5401 cttcggacag cagtatcatc gactatggag tcgagagcgc gctcgtcgac tatggagtcg
     5461 tcagcagtac tactgacgat ggagtcgaca gcagtgtgtg agtctatgga gtcgagagca
     5521 tagacatcga gtatggagtc gactacagtc gctacgacta tggagtcgac agagatatca
     5581 tgcagtatgg agtcgacagc agtatctgct gtcatggagt cgactgcacg acagtcgact
     5641 atggagtcgc gagcgctatg agcgactatg ggaattctcg acctcgagac aaatggcagc
     5701 ccgggggatc caaactcgag aaagctagca aaccatggta cgatgatgat ccagacatga
     5761 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     5821 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     5881 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     5941 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct ctagatcaga
     6001 tctcttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6121 ttatcatgtc tggatctacg taatacgact cactatagtt ctagaggctc gagaggggcc
     6181 gctttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
     6241 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
     6301 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
     6361 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
     6421 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
     6481 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
     6541 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
     6601 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
     6661 ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac
     6721 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
     6781 gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc
     6841 gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac
     6901 catggtcact gtctgctttg ctgttggtct gggctcctgg gtcactggct tactaatgga
     6961 gtctttatgt atgaggactc ttatcaattg ttcttctata aaggtctgca gtgtttctgt
     7021 tcgtccccta catggacacc cagagcctcc taaatacagg agccctgata actgcacaag
     7081 tgctcagatt ccagcagggt ggaaaatgag ataaagtgtg cagatgggga gggggacgtg
     7141 aatgagagat ttgagggatg aaaaggatgg atgaacgcat tgaaaatagc ccctttcaca
     7201 aaataatacc agtaaattgt catataatta atgaccagac ctttacctgt aaatgatgtg
     7261 atgttcatat acagaagaat gccaacaatt tacagatgat tttacaaatt cttaattttc
     7321 cttttctaaa tggatttgtt cacacattat ctcttcaaag caattgaatt ttctggaaaa
     7381 gattggctgt gtgtgtaaaa ggtgctactg aggaatttaa tgtgacatgg aatgaagcaa
     7441 acagcagcct aagacaggat ggcaggaaaa atgtcacatg tttaaaataa gagtgaaacc
     7501 aacgacctgg acttatagag actgtgtgtc cttaggcaaa gctcttgtac aaacagtgct
     7561 tgtgactgtg aaattcgggg tttgcctgga ttgtgttaaa tgtgtgcttg gatgtatcat
     7621 aaaaaagaat cggttctctg tatttgacat tttctgtaat ttatctaaag ttacaactgg
     7681 ctatgcctga tttttatcac tattgaatca atcattttaa agaatgttaa gtctgctgat
     7741 ttggagagct catttacatt tattttgaat gtctcttatt taacacaact gatttaagct
     7801 ttagatctaa tctgatcatt caggcgcgcc aacacgtgaa taccacgcga ggccttaggg
     7861 ataacagggt aatacgcgtg atctgcgaag atacggccac gggtgctctt gatcctgtgg
     7921 ctgattttgg actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt
     7981 ccactgattg actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta
     8041 atagatgtga catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat
     8101 aatgtgaaaa cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc
     8161 gcaattcaat tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata
     8221 agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa
     8281 atgtaattaa gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc
     8341 cccaaaaata atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct
     8401 ggttcttgac cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc
     8461 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     8521 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8581 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8641 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8701 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8761 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8821 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9121 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9361 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9481 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9541 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9601 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9661 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9721 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9781 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9841 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9901 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9961 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10021 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10081 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10141 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10201 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10261 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10321 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10381 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10441 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10501 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10561 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10621 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tg
//
