LOCUS       sequence-209081-        9978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human JMJD2B with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9978)
  AUTHORS   Cloos PA, Christensen J, Agger K, Maiolica A, Rappsilber J, Antal T,
            Hansen KH, Helin K
  TITLE     The putative oncogene GASC1 demethylates tri- and dimethylated 
            lysine 9 on histone H3.
  JOURNAL   Nature. 2006 Jul 20. 442(7100):307-11.
  PUBMED    16732293
REFERENCE   2  (bases 1 to 9978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2006 Jul 20. 442(7100):307-11."
COMMENT     Sequence Label: pCMV-HA-JMJD2B
FEATURES             Location/Qualifiers
     source          1..9978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(123..142)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    167..214
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        507..886
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        887..1090
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1041..1061
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1087..1111
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1202..1774
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     CDS             1854..1880
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1893..1917
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5283..5307)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5406..5425)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5471..5526
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5525..5544)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5884..5900)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5884..5900)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5897..5919)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5908..5924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5932..5938,5939..5956,5957..5962))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5977..5998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6115..6132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6286..6874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6366..6385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7045..7836,7837..7905))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7668..7687
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7906..8010)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8078..8096
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8134..8156)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8256..8275
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8469..8491
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8483..8500
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8484..8500
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8911..9056
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             9113..9907
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9167..9186)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9777..9796
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
       61 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccgggagatg ggggaggcta
      121 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
      181 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
      241 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
      301 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
      361 gcggcaagcc ctgccatagc cacgggcccc gtgggttagg gacggcggat cgcggccctc
      421 tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
      481 ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc
      541 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      601 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      661 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      721 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      781 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      841 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      901 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      961 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     1021 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     1081 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     1141 cctccataga agacaccggg accgatccag cctccggtcg accgatcctg agaacttcag
     1201 ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata
     1261 tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca
     1321 tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac cattgtctcc
     1381 tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc atttgtaacg
     1441 aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct aatcactttt
     1501 ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga gaacaattgt
     1561 tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc gtggaaatat
     1621 tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt tatggttaca
     1681 atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg gcccctctgc
     1741 taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc tggttgttgt
     1801 gctgtctcat cattttggca aagaattcct cgacggatca attcaccatg gcatacccat
     1861 acgatgttcc agattacgct agcggatcat caacaagttt gtacaaaaaa gcaggctctt
     1921 taaaggaacc aattcagtcg actggatccg gtaccgccat ggggtctgag gaccacggcg
     1981 cccagaaccc cagctgtaaa atcatgacgt ttcgcccaac catggaagaa tttaaagact
     2041 tcaacaaata cgtggcctac atagagtcgc agggagccca ccgggcgggc ctggccaaga
     2101 tcatcccccc gaaggagtgg aagccgcggc agacgtatga tgacatcgac gacgtggtga
     2161 tcccggcgcc catccagcag gtggtgacgg gccagtcggg cctcttcacg cagtacaata
     2221 tccagaagaa ggccatgaca gtgggcgagt accgccgcct ggccaacagc gagaagtact
     2281 gtaccccgcg gcaccaggac tttgacgacc ttgaacgcaa atactggaag aacctcacct
     2341 ttgtctcccc gatctacggg gctgacatca gcggctcttt gtatgatgac gacgtggccc
     2401 agtggaacat cgggagcctc cggaccatcc tggacatggt ggagcgcgag tgcggcacca
     2461 tcatcgaggg cgtgaacacg ccctacctgt acttcggcat gtggaagacc accttcgcct
     2521 ggcacaccga ggacatggac ctgtacagca tcaactacct gcactttggg gagcctaagt
     2581 cctggtacgc catcccacca gagcacggca agcgcctgga gcggctggcc atcggcttct
     2641 tccccgggag ctcgcagggc tgcgacgcct tcctgcggca taagatgacc ctcatctcgc
     2701 ccatcatcct gaagaagtac gggatcccct tcagccggat cacgcaggag gccggggaat
     2761 tcatgatcac atttccctac ggctaccacg ccggcttcaa tcacgggttc aactgcgcag
     2821 aatctaccaa cttcgccacc ctgcggtgga ttgactacgg caaagtggcc actcagtgca
     2881 cgtgccggaa ggacatggtc aagatctcca tggacgtgtt cgtgcgcatc ctgcagcccg
     2941 agcgctacga gctgtggaag cagggcaagg acctcacggt gctggaccac acgcggccca
     3001 cggcgctcac cagccccgag ctgagctcct ggagtgcatc ccgggcctcg ctgaaggcca
     3061 agctcctccg caggtcccac cggaaacgga gccagcccaa gaagccgaag cccgaagacc
     3121 ccaagttccc tggggagggt acggctgggg cagcgctcct agaggaggct gggggcagcg
     3181 tgaaggagga ggctgggccg gaggttgacc ccgaggagga ggaggaggag ccgcagccac
     3241 tgccacacgg ccgggaggcc gagggcgcag aagaggacgg gaggggcaag ctgcggccaa
     3301 ccaaggccaa gagcgagcgg aagaagaaga gcttcggcct gctgccccca cagctgccgc
     3361 ccccgcctgc tcacttcccc tcagaggagg cgctgtggct gccatcccca ctggagcccc
     3421 cggtgctggg cccaggccct gcagccatgg aggagagccc cctgccggca ccccttaatg
     3481 tcgtgccccc tgaggtgccc agtgaggagc tagaggccaa gcctcggccc atcatcccca
     3541 tgctgtacgt ggtgccgcgg ccgggcaagg cagccttcaa ccaggagcac gtgtcctgcc
     3601 agcaggcctt tgagcacttt gcccagaagg gtccgacctg gaaggaacca gtttccccca
     3661 tggagctgac ggggccagag gacggtgcag ccagcagtgg ggcaggtcgc atggagacca
     3721 aagcccgggc cggagagggg caggcaccgc ccacattttc caaattgaag atggagatca
     3781 agaagagccg gcgccatccc ctgggccggc cgcccacccg gtccccactg tcggtggtga
     3841 agcaggaggc ctcaagtgac gaggaggcat cccctttctc cggggaggaa gatgtgagtg
     3901 acccggacgc cttgaggccg ctgctgtctc tgcagtggaa gaacagggcg gccagcttcc
     3961 aggccgagag gaagttcaac gcagcggctg cgcgcacgga gccctactgc gccatctgca
     4021 cgctcttcta cccctactgc caggccctac agactgagaa ggaggcaccc atagcctccc
     4081 tcggagaggg ctgcccggcc acattaccct ccaaaagccg tcagaagacc cgaccgctca
     4141 tccctgagat gtgcttcacc tctggcggta agaacacgga gccgctgcct gccaactcct
     4201 acatcggcga cgacgggacc agccccctga tcgcctgcgg caagtgctgc ctgcaggtcc
     4261 atgccagttg ctatggcatc cgtcccgagc tggtcaatga aggctggacg tgttcccggt
     4321 gcgcggccca cgcctggact gcggaatgct gcctgtgcaa cctgcgagga ggtgcgctgc
     4381 agatgaccac cgataggagg tggatccacg tgatctgtgc catcgcagtc cccgaggcgc
     4441 gcttcctgaa cgtgattgag cgccaccctg tggacatcag cgccatcccc gagcagcggt
     4501 ggaagctgaa atgcgtgtac tgccggaagc ggatgaagaa ggtgtcaggt gcctgtatcc
     4561 agtgctccta cgagcactgc tccacgtcct tccacgtgac ctgcgcccac gccgcaggcg
     4621 tgctcatgga gccggacgac tggccctatg tggtctccat cacctgcctc aagcacaagt
     4681 cggggggtca cgctgtccaa ctcctgaggg ccgtgtccct aggccaggtg gtcatcacca
     4741 agaaccgcaa cgggctgtac taccgctgtc gcgtcatcgg tgccgcctcg cagacctgct
     4801 acgaagtgaa cttcgacgat ggctcctaca gcgacaacct gtaccctgag agcatcacga
     4861 gtagggactg tgtccagctg ggaccccctt ccgaggggga gctggtggag ctccggtgga
     4921 ctgacggcaa cctctacaag gccaagttca tctcctccgt caccagccac atctaccagg
     4981 tggagtttga ggacgggtcc cagctgacgg tgaagcgtgg ggacatcttc accctggagg
     5041 aggagctgcc caagagggtc cgctctaggc tgtcactgag cacgggggca ccgcaggagc
     5101 ccgccttctc gggggaggag gccaaggccg ccaagcgccc gcgtgtgggc accccgcttg
     5161 ccacggagga ctccgggcgg agccaggact acgtggcctt cgtggagagc ctcctgcagg
     5221 tgcagggccg gcccggagcc cccttctagg acagctggcc gctcagcact cgagatatct
     5281 agacccagct ttcttgtaca aagtggttga tgatccgtcg aggaattcac tcctcaggtg
     5341 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac
     5401 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     5461 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     5521 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     5581 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa
     5641 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     5701 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     5761 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     5821 gtcatagctg tccctcttct cttatggaga tccctcgacg gatcggccgc aattcgtaat
     5881 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     5941 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6001 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6061 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6721 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7021 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7081 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7141 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7201 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     7261 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     7321 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     7381 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7441 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7501 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7561 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7621 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7681 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7741 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7801 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7861 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7921 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7981 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8041 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     8101 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     8161 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     8221 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     8281 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     8341 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     8401 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     8461 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttc acgctgccgc
     8521 aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc cagtccgcag
     8581 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca
     8641 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg
     8701 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg
     8761 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga
     8821 tcaagatcct gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa
     8881 gaaatatatt tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca
     8941 ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat
     9001 attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaacagc
     9061 gtcaacagcg tgccgcagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     9121 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     9181 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     9241 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     9301 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     9361 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     9421 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     9481 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     9541 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     9601 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     9661 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     9721 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     9781 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     9841 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     9901 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     9961 cgagatttcg attccacc
//
