LOCUS       sequence-209082-        4351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Lambert PF, Spalholz BA, Howley PM
  TITLE     A transcriptional repressor encoded by BPV-1 shares a common 
            carboxy-terminal domain with the E2 transactivator.
  JOURNAL   Cell. 1987 Jul 3. 50(1):69-78.
  PUBMED    3036366
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1987 
            Jul 3. 50(1):69-78."
COMMENT     Sequence Label: p1153 BPV-1 E2-TR
FEATURES             Location/Qualifiers
     source          1..4351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          32..128
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    1419..1553
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1443..1462)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1497..1516
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1696..1718)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1804..1944
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1859..1878
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1959..1976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2130..2718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2210..2229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2889..3680,3681..3749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3512..3531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3750..3854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3922..3940
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4010..4030)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4015..4344
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4195..4330
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tcgaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt cttttatttc
       61 aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga tgttgccttt
      121 acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgctgcag gggggggggg
      181 gggggggatt tgtacatgcg cacagaggac ggctggcagc ttgcgaaggc tggggctgac
      241 ggaactgggc tctactactg caccatggcc ggtgctggac gcatttacta ttctcgcttt
      301 ggtgacgagg cagccagatt tagtacaaca gggcattact ctgtaagaga tcaggacaga
      361 gtgtatgctg gtgtctcatc cacctcttct gattttagag atcgcccaga cggagtctgg
      421 gtcgcatccg aaggacctga aggagaccct gcaggaaaag aagccgagcc agcccagcct
      481 gtctcttctt tgctcggctc ccccgcctgc ggtcccatca gagcaggcct cggttgggta
      541 cgggacggtc ctcgctcgca cccctacaat tttcctgcag gctcgggggg ctctattctc
      601 cgctcttcct ccaccccggt gcagggcacg gtaccggtgg acttggcatc aaggcaggaa
      661 gaagaggagc agtcgcccga ctccacagag gaagaaccag tgactctccc aaggcgcacc
      721 accaatgatg gattccacct gttaaaggca ggagggtcat gctttgctct aatttcagga
      781 actgctaacc aggtaaagtg ctatcgcttt cgggtgaaaa agaaccatag acatcgctac
      841 gagaactgca ccaccacctg gttcacagtt gctgacaacg gtgctgaaag acaaggacaa
      901 gcacaaatac tgatcacctt tggatcgcca agtcaaaggc aagactttct gaaacatgta
      961 ccactacctc ctggaatgaa catttccggc tttacagcca gcttggactt ctgatcactg
     1021 ccattgcctt ttcttcatct gactggtgta ctatgccaaa tctatggttt ctattgttct
     1081 tgggactagt tgctgcaatg caactgctgc tattactgtt cttactcttg ttttttcttg
     1141 tatactggga tcattttgag tgctcctgta caggtctgcc cttttaatgc ctttacatca
     1201 ctggctattg gctgtgtttt tactgttgtg tggatttgat ttgttttata tactgtatga
     1261 agttttttca tttgtgcttg tattgctgtt tgtaagtttt ttactagagt ttgtattccc
     1321 cctgctcaga ttttatatgg tttaagctgc agcaataaaa atgagtgcac ggaaaaggta
     1381 ccttctgagg cggaaagaac cagccggatc cctcgaggga tccagacatg ataagataca
     1441 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1501 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1561 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1621 agtaaaacct ctacaaatgt ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg
     1681 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     1741 agcggatgcc grgagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     1801 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     1861 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     1921 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1981 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     2041 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     2101 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     2161 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     2221 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     2281 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     2341 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2401 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2461 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2521 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     2581 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2641 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2701 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2761 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2821 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2881 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2941 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3001 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     3061 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3181 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg
     3241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3481 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta
     3541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3841 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3901 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattg
     3961 gtcgaccaat tctcatgttt gacagcttat catcgataag cttggctgtg gaatgtgtgt
     4021 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4081 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4141 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     4201 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     4261 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4321 tttggaggcc taggcttttg caaaaagctc c
//
