LOCUS       sequence-209092-        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Minimal ETS domain of human ETV6 (TEL).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Vo T, Wang S, Poon GMK, Wilson WD
  TITLE     Electrostatic control of DNA intersegmental translocation by the ETS
            transcription factor ETV6.
  JOURNAL   J Biol Chem. 2017 Aug 11;292(32):13187-13196. doi: 
            10.1074/jbc.M117.792887. Epub 2017 Jun 7.
  PUBMED    28592487
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.792887"; journalName: "J Biol Chem"; date: 
            "2017-08-11- 11"; volume: "292"; issue: "32"; pages: "13187-13196"
COMMENT     Sequence Label: pET28b-ETV6-ETS
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             340..357
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             358..375
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             385..402
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(455..473)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      469..516
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      553..1008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(640..659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     850..871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttgagaggaa ttccctctag aatattttgt ttactttaaa gaaggagata taccatgggg
       61 agaatagcag actgtagact gctttgggat tacgtctatc agttgctttc tgacagccgg
      121 tacgaaaact tcatccgatg ggaggacaaa gaatccaaaa tattccggat agtggatccc
      181 aacggactgg ctcgactgtg gggaaaccat aagaacagaa caaacatgac ctatgagaaa
      241 atgtccagag ccctgcgcca ctactacaaa ctaaacatta tcaggaagga gccaggacaa
      301 aggcttttgt tcaggtttat gaaaacccca gatgaaatcc tagtgccacg aggttcccat
      361 caccatcacc atcactaact cgagcaccac caccaccacc actgagatcc ggctgctaac
      421 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      481 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      541 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      601 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      661 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      721 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      781 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      841 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
      901 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
      961 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     1021 tcggggaaat gtgcgcggaa cccctattt
//
