LOCUS       sequence-209101-        7017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  fluorescent fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Birbach A, Gold P, Binder BR, Hofer E, de Martin R, Schmid JA
  TITLE     Signaling molecules of the NF-kappa B pathway shuttle constitutively
            between cytoplasm and nucleus.
  JOURNAL   J Biol Chem. 2002 Mar 29;277(13):10842-51. doi: 
            10.1074/jbc.M112475200. Epub 2002 Jan 18.
  PUBMED    11801607
REFERENCE   2  (bases 1 to 7017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112475200"; journalName: "J Biol Chem"; date: 
            "2002-03-29- 29"; volume: "277"; issue: "13"; pages: "10842-51"
COMMENT     Sequence Label: IKK2-EYFP
FEATURES             Location/Qualifiers
     source          1..7017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2900..2909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2906..2908,2909..2911,2912..3622)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2951..2972)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3212..3231)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3559..3580
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3623..3688
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    3805..3926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3842..3861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3896..3915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3933..4388)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4070..4091)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4282..4301
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4415..4519
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4521..4878
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4521..4712
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4544..4564)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4729..4864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4913..5707
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4913..5707
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4967..4986)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5577..5596
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5895..5914)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5939..5986
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6315..6903
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6804..6823
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagactcg
      601 acttcgatat ctcgaattcg atatgagctg gtcaccttcc ctgacaacgc agacatgtgg
      661 ggcctgggaa atgaaagagc gccttgggac agggggattt ggaaatgtca tccgatggca
      721 caatcaggaa acaggtgagc agattgccat caagcagtgc cggcaggagc tcagcccccg
      781 gaaccgagag cggtggtgcc tggagatcca gatcatgaga aggctgaccc accccaatgt
      841 ggtggctgcc cgagatgtcc ctgaggggat gcagaacttg gcgcccaatg acctgcccct
      901 gctggccatg gagtactgcc aaggaggaga tctccggaag tacctgaacc agtttgagaa
      961 ctgctgtggt ctgcgggaag gtgccatcct caccttgctg agtgacattg cctctgcgct
     1021 tagatacctt catgaaaaca gaatcatcca tcgggatcta aagccagaaa acatcgtcct
     1081 gcagcaagga gaacagaggt taatacacaa aattattgac ctaggatatg ccaaggagct
     1141 ggatcagggc agtctttgca catcattcgt ggggaccctg cagtacctgg ccccagagct
     1201 actggagcag cagaagtaca cagtgaccgt cgactactgg agcttcggca ccctggcctt
     1261 tgagtgcatc acgggcttcc ggcccttcct ccccaactgg cagcccgtgc agtggcattc
     1321 aaaagtgcgg cagaagagtg aggtggacat tgttgttagc gaagacttga atggaacggt
     1381 gaagttttca agctctttac cctaccccaa taatcttaac agtgtcctgg ctgagcgact
     1441 ggagaagtgg ctgcaactga tgctgatgtg gcacccccga cagaggggca cggatcccac
     1501 gtatgggccc aatggctgct tcaaggccct ggatgacatc ttaaacttaa agctggttca
     1561 tatcttgaac atggtcacgg gcaccatcca cacctaccct gtgacagagg atgagagtct
     1621 gcagagcttg aaggccagaa tccaacagga cacgggcatc ccagaggagg accaggagct
     1681 gctgcaggaa gcgggcctgg cgttgatccc cgataagcct gccactcagt gtatttcaga
     1741 cggcaagtta aatgagggcc acacattgga catggatctt gtttttctct ttgacaacag
     1801 taaaatcacc tatgagactc agatctcccc acggccccaa cctgaaagtg tcagctgtat
     1861 ccttcaagag cccaagagga atctcgcctt cttccagctg aggaaggtgt ggggccaggt
     1921 ctggcacagc atccagaccc tgaaggaaga ttgcaaccgg ctgcagcagg gacagcgagc
     1981 cgccatgatg aatctcctcc gaaacaacag ctgcctctcc aaaatgaaga attccatggc
     2041 ttccatgtct cagcagctca aggccaagtt ggatttcttc aaaaccagca tccagattga
     2101 cctggagaag tacagcgagc aaaccgagtt tgggatcaca tcagataaac tgctgctggc
     2161 ctggagggaa atggagcagg ctgtggagct ctgtgggcgg gagaacgaag tgaaactcct
     2221 ggtagaacgg atgatggctc tgcagaccga cattgtggac ttacagagga gccccatggg
     2281 ccggaagcag gggggaacgc tggacgacct agaggagcaa gcaagggagc tgtacaggag
     2341 actaagggaa aaacctcgag accagcgaac tgagggtgac agtcaggaaa tggtacggct
     2401 gctgcttcag gcaattcaga gcttcgagaa gaaagtgcga gtgatctata cgcagctcag
     2461 taaaactgtg gtttgcaagc agaaggcgct ggaactgttg cccaaggtgg aagaggtggt
     2521 gagcttaatg aatgaggatg agaagactgt tgtccggctg caggagaagc ggcagaagga
     2581 gctctggaat ctcctgaaga ttgcttgtag caaggtccgt ggtcctgtca gtggaagccc
     2641 ggatagcatg aatgcctctc gacttagcca gcctgggcag ctgatgtctc agccctccac
     2701 ggcctccaac agcttacctg agccagccaa gaagagtgaa gaactggtgg ctgaagcaca
     2761 taacctctgc accctgctag aaaatgccat acaggacact gtgagggaac aagaccagag
     2821 tttcacggcc ctagactgga gctggttaca gacggaagaa gaagagcaca gctgcctgga
     2881 gcagggggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     2941 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3001 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3061 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt
     3121 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3181 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3241 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3301 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3361 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     3421 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3481 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     3541 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3601 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc
     3661 agtcgacggt accgcgggcc cgggatccac cggatctaga tcataatcag ccataccaca
     3721 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     3781 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     3841 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     3901 ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa
     3961 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4021 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4081 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4141 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4201 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4261 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4321 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4381 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4501 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     4561 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4621 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4681 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4741 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4801 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4861 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     4921 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4981 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     5041 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     5101 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     5161 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     5221 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     5281 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5341 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5401 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     5461 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5521 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5581 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5641 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5701 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     5761 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     5821 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     5881 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     5941 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     6001 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     6061 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     6121 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     6181 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6241 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6301 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6361 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6421 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6481 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6541 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6601 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6661 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6721 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6781 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6841 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6901 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6961 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
//
