LOCUS       sequence-209114-        9444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Worseck JM, Grossmann A, Weimann M, Hegele A, Stelzl U
  TITLE     A stringent yeast two-hybrid matrix screening approach for 
            protein-protein interaction discovery.
  JOURNAL   Methods Mol Biol. 2012;812:63-87. doi: 10.1007/978-1-61779-455-1_4.
  PUBMED    22218854
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2012"; volume: "812:63-87. doi"; pages: " 
            10.1007/978-1-61779-455-1_4"
COMMENT     Sequence Label: pACT4-DM
FEATURES             Location/Qualifiers
     source          1..9444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(65..84)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(744..1535,1536..1604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1367..1386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1605..1709)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1972..3314
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    2304..2351
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        3791..4195
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             4208..5302
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     protein_bind    5803..5836
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(5854..6041)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     5962..5986
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    6424..6548
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6573..6578,6579..6596,6597..6603)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6657..7316
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6723..6742)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7658..7963
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7842..7861
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8004..8128)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8142..8483)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             complement(8499..8519)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(8565..8966)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     primer_bind     complement(8995..9017)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9103..9243
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9158..9177
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9258..9275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9429..9444,1..573))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
       61 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      121 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      181 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      241 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      301 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      361 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
      421 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
      481 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
      541 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
      601 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
      661 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
      721 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
      781 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
      841 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
      901 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
      961 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1021 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     1081 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1141 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1201 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1261 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1321 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     1381 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     1441 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     1501 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     1561 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     1621 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     1681 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgctttg
     1741 aagaaaaatg cgccttattc aatctttgct ataaaaaatg gcccaaaatc tcacattgga
     1801 agacatttga tgacctcatt tctttcaatg aagggcctaa cggagttgac taatgttgtg
     1861 ggaaattgga gcgataagcg tgcttctgcc gtggccagga caacgtatac tcatcagata
     1921 acagcaatac ctgatcacta cttcgcacta gtttctcggt actatgcata tgatccaata
     1981 tcaaaggaaa tgatagcatt gaaggatgag actaatccaa ttgaggagtg gcagcatata
     2041 gaacagctaa agggtagtgc tgaaggaagc atacgatacc ccgcatggaa tgggataata
     2101 tcacaggagg tactagacta cctttcatcc tacataaata gacgcatata agtacgcatt
     2161 taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag gcaacacgca
     2221 gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc attttcggaa
     2281 gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc tctagaaagt
     2341 ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa
     2401 ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac
     2461 attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc
     2521 atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat
     2581 ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga
     2641 aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa
     2701 ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag
     2761 tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc
     2821 acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga
     2881 agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag
     2941 ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt
     3001 gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct
     3061 ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt
     3121 ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag
     3181 attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc
     3241 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc
     3301 acagatgctt cgttaacaaa gatatgctat tgaagtgcaa gatggaaacg cagaaaatga
     3361 accggggatg cgacgtgcaa gattacctat gcaatagatg caatagtttc tccaggaacc
     3421 gaaatacata cattgtcttc cgtaaagcgc tagactatat attattatac aggttcaaat
     3481 atactatctg tttcagggaa aactcccagg ttcggatgtt caaaattcaa tgatgggtaa
     3541 caagtacgat cgtaaatctg taaaacagtt tgtcggatat taggctgtat ctcctcaaag
     3601 cgtattcgaa tatcattgag aagctgcagc gtcacatcgg ataataatga tggcagccat
     3661 tgtagaagtg ccttttgcat ttctagtctc tttctcggtc tagctagttt tactacatcg
     3721 cgaagataga atcttagatc acactgcctt tgctgagctg gatcaataga gtaacaaaag
     3781 agtggtaagg aactgtggga atactcaggt atcgtaagat gcaagagttc gaatctctta
     3841 gcaaccatta tttttttcct caacataacg agaacacaca ggggcgctat cgcacagaat
     3901 caaattcgat gactggaaat tttttgttaa tttcagaggt cgcctgacgc atataccttt
     3961 ttcaactgaa aaattgggag aaaaaggaaa ggtgagaggc cggaaccggc ttttcatata
     4021 gaatagagaa gcgttcatga ctaaatgctt gcatcacaat acttgaagtt gacaatatta
     4081 tttaaggacc tattgttttt tccaataggt ggttagcaat cgtcttactt tctaactttt
     4141 cttacctttt acatttcagc aatatatata tatatttcaa ggatatacca ttctaatgtc
     4201 tgcccctatg tctgccccta agaagatcgt cgttttgcca ggtgaccacg ttggtcaaga
     4261 aatcacagcc gaagccatta aggttcttaa agctatttct gatgttcgtt ccaatgtcaa
     4321 gttcgatttc gaaaatcatt taattggtgg tgctgctatc gatgctacag gtgtcccact
     4381 tccagatgag gcgctggaag cctccaagaa ggttgatgcc gttttgttag gtgctgtggg
     4441 tggtcctaaa tggggtaccg gtagtgttag acctgaacaa ggtttactaa aaatccgtaa
     4501 agaacttcaa ttgtacgcca acttaagacc atgtaacttt gcatccgact ctcttttaga
     4561 cttatctcca atcaagccac aatttgctaa aggtactgac ttcgttgttg tcagagaatt
     4621 agtgggaggt atttactttg gtaagagaaa ggaagacgat ggtgatggtg tcgcttggga
     4681 tagtgaacaa tacaccgttc cagaagtgca aagaatcaca agaatggccg ctttcatggc
     4741 cctacaacat gagccaccat tgcctatttg gtccttggat aaagctaatg ttttggcctc
     4801 ttcaagatta tggagaaaaa ctgtggagga aaccatcaag aacgaatttc ctacattgaa
     4861 ggttcaacat caattgattg attctgccgc catgatccta gttaagaacc caacccacct
     4921 aaatggtatt ataatcacca gcaacatgtt tggtgatatc atctccgatg aagcctccgt
     4981 tatcccaggt tccttgggtt tgttgccatc tgcgtccttg gcctctttgc cagacaagaa
     5041 caccgcattt ggtttgtacg aaccatgcca cggttctgct ccagatttgc caaagaataa
     5101 ggttgaccct atcgccacta tcttgtctgc tgcaatgatg ttgaaattgt cattgaactt
     5161 gcctgaagaa ggtaaggcca ttgaagatgc agttaaaaag gttttggatg caggtatcag
     5221 aactggtgat ttaggtggtt ccaacagtac caccgaagtc ggtgatgctg tcgccgaaga
     5281 agttaagaaa atccttgctt aaaaagattc tcttttttta tgatatttgt acataaactt
     5341 tataaatgaa attcataata gaaacgacac gaaattacaa aatggaatat gttcataggg
     5401 tagacgaaac tatatacgca atctacatac atttatcaag aaggagaaaa aggaggatag
     5461 taaaggaata caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt
     5521 gcactttaac attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag
     5581 aaaatcatga aactacgatt cctaatttga tattggagga ttttctctaa aaaaaaaaaa
     5641 atacaacaaa taaaaaacac tcaatgacct gaccatttga tggagtttaa gtcaatacct
     5701 tcttgaacca tttcccataa tggtgaaagt tccctcaaga attttactct gtcagaaacg
     5761 gccttaacga cgtagtcgag gccgcgatcc ggaaccctta atataacttc gtataatgta
     5821 tgctatacga agttattagg tccctcgcac atgccggtag aggtgtggtc aataagagcg
     5881 acctcatgct atacctgaga aagcaacctg acctacagga aagagttact caagaacaag
     5941 aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat tttttttata
     6001 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtgtcaaca
     6061 acgtatctac caacgatttg acccttttcc atcttttcgt aaatttctgg caaggtagac
     6121 aagccgacaa ccttgattgg agacttgacc aaacctctgg cgaagaagtc caaagcttct
     6181 gaataagccc tcgtaatata ttttcatgaa gaatttaggt ccaaaaaaaa gatgggcatt
     6241 aattctagtc atttaaaaaa ttctatagat cagaggttac atggccaaga ttgaaactta
     6301 gaggagtata gttacataaa agaaggcaaa acgatgtata aatgaaagaa attgagatgg
     6361 tgcacgatgc acagttgaag tgaacttgcg gggtttttca gtatctacga ttcatagatc
     6421 tccacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
     6481 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     6541 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     6601 atgtgtggat tttgagttag gatccggcga gattttcagg agctaaggaa gctaaaatgg
     6661 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     6721 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     6781 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     6841 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     6901 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     6961 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     7021 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     7081 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     7141 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     7201 aggtgctgat gccgctggcg attcaggttc atcatgccgt ctgtgatggc ttccatgtcg
     7261 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaaagat
     7321 ctggatccgg cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg
     7381 gtataagaat atatactgat atgtataccc gaagtatgtc aaaaagaggt gtgctatgaa
     7441 gcagcgtatt acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat
     7501 gtcaatatct ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc
     7561 gaacgctgga aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg
     7621 aacggctctt ttgctgacga gaacagggac tggtgaaatg cagtttaagg tttacaccta
     7681 taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc
     7741 cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg
     7801 tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat
     7861 ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa
     7921 tgacatcaaa aacgccatta acctgatgtt ctggggaata taaatgtcag gctccgttat
     7981 acacagccag tctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg
     8041 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     8101 ctcgttcagc tttcttgtac aaagtggtaa gtaattaaga tctctttttt tgggtttggt
     8161 ggggtatctt catcatcgaa tagatagtta tatacatcat ccattgtagt ggtattaaac
     8221 atccctgtag tgattccaaa cgcgttatac gcagtttggt ccgtccaacc aggtgacagt
     8281 ggttttgaat tattaccatc atcaatttta ctagccgtga tttcattatt catgaagtta
     8341 tcatgaacgt tagaggaggc aattggttgt gaaagcgctt gagaatttgt ttgagttgtt
     8401 atgaggttcg gaccgttgct actgttagtg aaagtgaagg acaatgagct atcagcaata
     8461 ttcccacttt gattaaaatt ggcggcggta cccaattcga cctttctctt cttttttgga
     8521 ggctcgggaa ttaattccgc tttatccatc tttgcaaagc ttggagttga ttgtatgctt
     8581 ggtatagctt gaaatattgt gcagaaaaag aaacaaggaa gaaagggaac gagaacaatg
     8641 acgaggaaac aaaagattaa taattgcagg tctatttata cttgatagca aagcggcaaa
     8701 ctttttttat ttcaaattca agtaactgga aggaaggccg tataccgttg ctcattagag
     8761 agtagtgtgc gtgaatgaag gaaggaaaaa gtttcgtgtg ttcgaagata cccctcatca
     8821 gctctggaac aacgacatct gttggtgctg tctttgtcgt taattttttc ctttagtgtc
     8881 ttccatcatt ttttttgtca ttgcggatat ggtgagacaa caacggggga gagagaaaag
     8941 aaaaaaaaag aaaagaagtt gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     9001 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     9061 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     9121 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     9181 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     9241 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9301 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9361 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9421 ctggcgtttt tccataggct ccgc
//
