LOCUS       sequence-209129-        8630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Broad host-range bacterial expression vector with constitutive Pc 
            promoter followed by the E. coli TorA signal peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8630)
  AUTHORS   Wilton R, Ahrendt AJ, Shinde S, Sholto-Douglas DJ, Johnson JL, 
            Brennan MB, Kemner KM
  TITLE     A New Suite of Plasmid Vectors for Fluorescence-Based Imaging of 
            Root Colonizing Pseudomonads.
  JOURNAL   Front Plant Sci. 2018 Feb 1;8:2242. doi: 10.3389/fpls.2017.02242. 
            eCollection 2017.
  PUBMED    29449848
REFERENCE   2  (bases 1 to 8630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2018-02-1"; volume: "8:2242. doi"; pages: " 
            10.3389/fpls.2017.02242. eCollection 2017"
COMMENT     Sequence Label: pSW002-Pc-TorA(sp)
FEATURES             Location/Qualifiers
     source          1..8630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1179..1808
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2237..3310
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3376..3570
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     rep_origin      4279..4824
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     4725..4744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4941..4958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     RBS             5606..5628
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(6632..7282)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             7388..8587
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
ORIGIN
        1 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
       61 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
      121 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
      181 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
      241 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
      301 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
      361 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
      421 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
      481 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
      541 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
      601 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
      661 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
      721 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
      781 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
      841 ggcagccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
      901 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
      961 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     1021 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     1081 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     1141 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     1201 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     1261 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     1321 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     1381 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     1441 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     1501 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     1561 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     1621 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     1681 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     1741 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     1801 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     1861 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     1921 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     1981 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     2041 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     2101 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     2161 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
     2221 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     2281 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     2341 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcagcc gctgatcgaa tccgcaaaga
     2401 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     2461 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     2521 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     2581 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     2641 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     2701 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     2761 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     2821 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     2881 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     2941 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     3001 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     3061 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     3121 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     3181 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     3241 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     3301 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     3361 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     3421 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     3481 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     3541 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     3601 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     3661 tatcgcggcc gatggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     3721 cggacaagcc gcgccgtcgc cactcgaccg ccggcgctga ggtctgcctc gtgaagaagg
     3781 tgttgctgac tcataccagg caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3841 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3901 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3961 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4021 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4081 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4141 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4201 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4261 cttaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa
     4321 cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa
     4381 ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta
     4441 accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg
     4501 gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc
     4561 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
     4621 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
     4681 aaccgaaagg caggaacagg agagcgcacg agggagccgc cagggggaaa cgcctggtat
     4741 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     4801 tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg ccctctcact tccctgttaa
     4861 gtatcttcct ggcatcttcc aggaaatctc cgccccgttc gtaagccatt tccgctcgcc
     4921 gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaatat atcctgtatc
     4981 acatattctg ctgacgcacc ggtgcagcct tttttctcct gccacatgaa gcacttcact
     5041 gacaccctca tcagtgccaa catagtaagc cagtatacac tccgctagcg ctgaggtctg
     5101 cctcgtgaag aaggtgttgc tgactcatac caggtagtcc gaggagcttt atgcttgtaa
     5161 accgttttgt gaaaaaattt ttaaaataaa aaaggggacc tctagggtcc ccaattaatt
     5221 agtaatataa tctattaaag gtcattcaaa aggtcatcca ccggatcaat tcccctgctc
     5281 gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc cgatccttgg agcccttgcc
     5341 ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc ttgacccgca gttgcaaacc
     5401 ctcactgatc cgctagtccg aggcctcgag atctatcgat gcatgccatg gtacccggga
     5461 gctcgaattc gccgcttgcg ccgcagtcgc acgtcgcggc gttaacaatg acggccccag
     5521 catcccggcg acggttaagc cgccgagttg tgccagaaaa cgccgacgtg atgcctgaaa
     5581 gagatcgtta ttgttcatat gtatatctcc ttcttaaagt taaacaaaat tatttcttga
     5641 acgggttcgc tacctgcatt acagcctaca gaccgagaag gcttatgtct actgggccaa
     5701 ggcatttgtg ttgtggacgg cccgcagcct tggtgggttt cgacatccgc gcgaaatggg
     5761 gcaagctgaa gtcgagggtt ttctgaccat gctcgccacc gagaagcaag tggcgccggc
     5821 cacccaccgg caggcgctca acgcgctgtt gttcttgtat cggcaacgcg tcgacgtcat
     5881 atggatccga tatcgccgtg gcggccagct tttgccattc tcaccggatt cagtcgtcac
     5941 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     6001 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     6061 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     6121 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     6181 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt
     6241 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca
     6301 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct
     6361 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat
     6421 gagtcagcaa caccttcttc acgaggcaga cctcagcgcc agaaggccgc cagagaggcc
     6481 gagcgcggcc gtgaggcttg gacgctaggg cagggcatga aaaagcccgt agcgggctgc
     6541 tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg tctacatggc tctgctgtag
     6601 tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc accctttctc ggtccttcaa
     6661 cgttcctgac aacgagcctc cttttcgcca atccatcgac aatcaccgcg agtccctgct
     6721 cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg
     6781 agagcggagc ctgttcaacg gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct
     6841 cctcaagcac ggccccaaca gtgaagtagc tgattgtcat cagcgcattg acggcgtccc
     6901 cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg
     6961 gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct
     7021 gcctgaagct gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca
     7081 ccgaatgcgt atgattctcc gccagcatgg cttcggccag tgcgtcgagc agcgcccgct
     7141 tgttcctgaa gtgccagtaa agcgccggct gctgaacccc caaccgttcc gccagtttgc
     7201 gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc cagggcggca cggatcactg
     7261 tattcggctg caactttgtc atgcttgaca ctttatcact gataaacata atatgtccac
     7321 caacttatca gtgataaaga atccgcgcgt tcaatcggac cagcggaggc tggtccggag
     7381 gccagacgtg aaacccaaca tacccctgat cgtaattctg agcactgtcg cgctcgacgc
     7441 tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc tggttcactc
     7501 gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttgg tgcaatttgc
     7561 ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt
     7621 ctcgctggcc ggcgccactg tcgactacgc catcatggcg acagcgcctt tcctttgggt
     7681 tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg tagccggcgc
     7741 ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc
     7801 ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc
     7861 cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg
     7921 tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg aggctctcaa
     7981 cccgctcgct tcgttccggt gggcccgggg catgaccgtc gtcgccgccc tgatggcggt
     8041 cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca ttttcggcga
     8101 ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat ttggcattct
     8161 gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg
     8221 ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg ccttcgcgac
     8281 acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca tcggaatgcc
     8341 ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg
     8401 ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct tcacggcgat
     8461 ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta
     8521 cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga
     8581 tcgctgatcg tggaaacgat aggggccgct ctagtactag ctagaactat
//
