LOCUS       sequence-209161-         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A JNK biosensor based on fluorescence resonance energy transfer 
            (FRET) and those based on bioluminescence energy transfer (BRET).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Komatsu N, Terai K, Imanishi A, Kamioka Y, Sumiyama K, Jin T, Okada 
            Y, Nagai T, Matsuda M
  TITLE     A platform of BRET-FRET hybrid biosensors for optogenetics, chemical
            screening, and in vivo imaging.
  JOURNAL   Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.1038/s41598-018-27174-x.
  PUBMED    29895862
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-12"; volume: "8(1):8984. doi"; pages: " 
            10.1038/s41598-018-27174-x"
COMMENT     Sequence Label: pCAGGS-6020nes
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             252..599
                     /codon_start=1
                     /product="long flexible linker consisting of SAGG repeats 
                     (Komatsu et al., 2011)"
                     /label=EV linker
                     /translation="SAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGS
                     AGGSAGGSGSAGGSAGGSTSAGGSAGGSAGGSAGGSAGGSGSAGGSAGGSTSAGGSAGG
                     SAGGSAGGSAGG"
ORIGIN
        1 tttcaagact gtcaaataag catttccaaa tactactagg agaagacggt aaccttttat
       61 tgaatgacat ttccactaat gggacctggt taaatgggca aaaagtcgag aagaacagca
      121 atcagttact gtcccaaggt gatgaaataa ccgttggtgt aggcgtggaa tcagatattt
      181 tatctctggt cattttcata aacgacaaat ttaagcagtg cctggagcag aacaaagttg
      241 atcgcggtac cagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggta
      301 gtgctggtgg ttccggcagt gctggtggta gtgctggtgg tagtaccagt gctggtggta
      361 gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggttcc ggcagtgctg
      421 gtggtagtgc tggtggtagt accagtgctg gtggtagtgc tggtggtagt gctggtggta
      481 gtgctggtgg tagtgctggt ggttccggca gtgctggtgg tagtgctggt ggtagtacca
      541 gtgctggtgg tagtgctggt ggtagtgctg gtggtagtgc tggtggtagt gctggtggtt
      601 ccggagacag tgtcaagacc cccgaggatg aaggcaaccc actgctagag cagcttgaga
      661 agaa
//
