LOCUS       sequence-209163-       11242 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA tagged UBE3B deletion of IQ domain in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Braganza A, Li J, Zeng X, Yates NA, Dey NB, Andrews J, Clark J, 
            Zamani L, Wang XH, St Croix C, O'Sullivan R, Garcia-Exposito L, 
            Brodsky JL, Sobol RW
  TITLE     UBE3B is a Calmodulin-Regulated, Mitochondria-Associated E3 
            Ubiquitin Ligase.
  JOURNAL   J Biol Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824.
  PUBMED    28003368
REFERENCE   2  (bases 1 to 11242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Dec 21. pii: jbc.M116.766824. doi: 
            10.1074/jbc.M116.766824."
COMMENT     Sequence Label: pLVX-UBE3B-Delta-IQ-HA-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        24..327
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        328..531
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     482..502
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     528..552
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    647..671
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3713..3739
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3759..3783)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(3815..4389,4390..4392,4393..4401)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3982..3999)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4209..4228
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4421..5020
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4421..4440)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4917..4937
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5034..5622
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5087..5107)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5505..5516)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5829..6462
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6590..6606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6590..6606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6603..6625)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6614..6630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6638..6644,6645..6662,6663..6668))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6683..6704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6821..6838)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6992..7580)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7072..7091)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7751..8542,8543..8611))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8374..8393
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8612..8716)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8764..8898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8801..8820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8855..8874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9066..9699
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9746..9871
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10368..10601
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10786..10830
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11093..11208
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
       61 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      121 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      181 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      241 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      301 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      361 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      421 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      481 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      541 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
      601 ctctactaga ggatctattt ccggtgaatt cctcgagact agatcaacaa gtttgtacaa
      661 aaaagcaggc tccgcggccg cccccttcac catggcagat gaccctgagt ccactaaaag
      721 aagtgcactt tgtattttca agattgccag gaaactgctg ttcctattca gaatcaaaga
      781 ggataatgag agatttgaga agttgtgtcg cagcatcctg agcagcatgg atgctgagaa
      841 tgagcctaag gtgtggtatg tgtccctggc ttgttctaag gacctcaccc tcctttggat
      901 tcaacagatc aagaacattt tgtggtactg ctgtgatttt ctcaagcagc tcaagcctga
      961 aatcctgcag gactcccgac tcatcaccct gtacctcacg atgcttgtca ccttcacaga
     1021 cacttcaacg tggaaaattc ttcggggaaa aggtgaaagt cttcgaccag cgatgaacca
     1081 catttgtgca aatataatgg gacatctcaa ccagcatgga ttttattctg tgctgcagat
     1141 attgttaacc cgtggcctgg caagaccccg tccttgtcta tccaaaggca ctttaacagc
     1201 agctttttct ctagcgttac gccctgtgat tgctgcacag ttctcagaca atctgattcg
     1261 gccgttcctc atccacatca tgtctgtgcc tgctctggtg actcatctca gcacagtgac
     1321 ccctgagcgc ctcactgttt tagaatccca tgacatgctt cgtaaattca tcatattttt
     1381 aagagaccaa gatcgatgcc gtgatgtatg tgaaagttta gaaggatgcc atacgctttg
     1441 tctaatgggc aacctcctac acttgggctc cctcagcccc agagtgttag aggaggagac
     1501 agatgggttc gtgagtttgc tcacccagac gctgtgctac tgtcggaagt atgtgtctca
     1561 gaagaagtcc aacctgaccc actggcatcc tgtccttggc tggttctccc aatctgtgga
     1621 ttatggcctt aacgagtcaa tgcacttgat caccaaacag ctgcagttct tgtggggggt
     1681 gcctctgatc cggatcttct tctgtgacat cctgagcaag aagctactgg agagccagga
     1741 gccagcccac gcacagccag catcccctca gaatgtgctc ccagtgaaga gtctcctaaa
     1801 gcgtgctttt caaaagtcgg catcagtccg gaatattctc aggcctgtcg ggggtaaacg
     1861 ggtcgactct gcagaagtcc ggaaggtttg caacatctgt gtcctctacc agacctcgct
     1921 gacaactctc acacagattc ggctgcagat actcacaggt ctcacttacc ttgatgacct
     1981 gcttcccaaa ctgtgggcat ttatctgtga gctcgggccc cacggagggt taaagctctt
     2041 cttggaatgc ctgaacaatg acactgaaga gtccaagcaa ctcttggcca tgctgatgct
     2101 gttctgtgac tgttcgcggc acctcatcac aatccttgat gacattgaag tttatgaaga
     2161 acagatttca ttcaaactgg aagagctggt cactatctcc tctttcctga attcttttgt
     2221 gtttaagatg atctgggatg gaattgtaga gaacgccaag ggtgagacct tggagctgtt
     2281 ccagtctgtc cacgggtggc ttatggtgct gtacgagcgg gactgccggc ggcgcttcac
     2341 ccccgaggac cactggctgc gaaaggatct caaacctagc gtgctcttcc aagaactcga
     2401 cagggacaga aaacgggcac agttgatcct gcagtacatc ccacatgtca tccctcacaa
     2461 aaacagagtt ctactgtttc gaaccatggt taccaaggag aaggagaaac tggggctggt
     2521 ggaaaccagc tctgcctccc cgcatgtcac tcacatcacc atccgccggt ccaggatgct
     2581 ggaggacggc tacgagcagc ttaggcagct ctcccagcac gccatgaagg gggtcatccg
     2641 tgtgaagttt gtcaatgacc tcggggtgga cgaagcaggg attgatcaag acggtgtttt
     2701 taaggagttc ttggaagaga tcatcaagag agtttttgac ccagcactca atctgttcaa
     2761 gacaaccagt ggggatgaga ggctgtaccc ctcacccaca tcctacatcc atgagaatta
     2821 cctgcagctc ttcgagtttg tggggaagat gctggggaag gctgtgtatg agggaattgt
     2881 ggtggacgtg ccatttgcat ccttcttcct gagccaactg cttgggcacc accacagcgt
     2941 cttctatagc tcggtggatg aactgccttc tctggactcc gagttctata aaaacctcac
     3001 ctccatcaag cgctatgatg gggacatcac tgacctgggc ctgacgctgt cttacgacga
     3061 ggacgtcatg ggtcagcttg tttgccatga actgattcct ggagggaaga ccattcctgt
     3121 tacaaatgaa aataaaatta gctacatcca tctgatggca cattttcgaa tgcacactca
     3181 aataaaaaac caaacagctg ccctcattag cggattccgt tccattatca aacccgagtg
     3241 gatccgaatg ttctcaactc ctgaactgca gcgtctcatc tctggcgaca atgctgagat
     3301 tgatctggaa gatttaaaga agcacacagt ctactacggt ggtttccatg gaagtcacag
     3361 agtcatcatc tggctctggg atattctggc ctccgacttc acaccggatg agagagctat
     3421 gtttctgaag ttcgtgacca gctgctccag acccccgctc ctgggattcg cctacctcaa
     3481 gcctccattc tccatccgct gcgtggaggt gtcggacgat caggacaccg gggacactct
     3541 gggcagcgtc ctccggggct tcttcaccat ccgcaagcgg gagccaggcg gccgcctgcc
     3601 cacctcctcc acctgcttca acctgctcaa gctgcccaac tacagcaaga agagcgtcct
     3661 ccgcgagaag ctgcgctacg ccatcagcat gaacacgggc tttgaactct cctacccata
     3721 cgatgttcca gattacgctt agaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     3781 ggttgatcta gttctagagc ggccgcggat cccgcccctc tccctccccc ccccctaacg
     3841 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     3901 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     3961 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     4021 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     4081 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     4141 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     4201 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     4261 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     4321 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     4381 gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt acaagcccac
     4441 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     4501 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     4561 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4621 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4681 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4741 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4801 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4861 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4921 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4981 accgcgcacc tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg aacaatcaac
     5041 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     5101 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     5161 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     5221 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     5281 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     5341 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     5401 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     5461 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     5521 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     5581 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
     5641 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     5701 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     5761 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5821 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     5881 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     5941 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     6001 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     6061 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     6121 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     6181 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     6241 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     6301 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6361 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6421 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6481 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     6541 attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6601 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6661 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6721 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6781 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6841 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6901 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6961 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7021 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7081 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7141 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7201 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7261 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7321 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7381 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7441 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7501 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7561 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7621 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7681 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7741 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7801 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7861 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7921 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7981 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8041 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8101 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8161 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8221 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8281 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8341 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8401 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8461 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8521 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8581 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8641 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8701 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     8761 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8821 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8881 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     8941 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     9001 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     9061 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     9121 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     9181 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     9241 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     9301 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     9361 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     9421 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     9481 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     9541 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9601 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9661 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     9721 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     9781 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     9841 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     9901 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     9961 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
    10021 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
    10081 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
    10141 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
    10201 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
    10261 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    10321 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    10381 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    10441 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    10501 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    10561 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    10621 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    10681 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    10741 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    10801 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    10861 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    10921 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    10981 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    11041 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg cctttaaaag
    11101 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
    11161 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
    11221 cagggacagc agagatccag tt
//
