LOCUS       sequence-209172-        6447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6447)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-TBRII-N-18
FEATURES             Location/Qualifiers
     source          1..6447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      655..664
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1991..2002
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2491..3171
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3293..3414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3330..3349)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3384..3403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3421..3876)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3558..3579)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3770..3789
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3903..4007
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4009..4366
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4009..4200
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4032..4052)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4217..4352
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4401..5195
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4401..5195
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4455..4474)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5065..5084
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5383..5402)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5427..5474
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5803..6391
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6292..6311
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgccacc
      661 atgggtcggg ggctgctcag gggcctgtgg ccgctgcaca tcgtcctgtg gacgcgtatc
      721 gccagcacga tcccaccgca cgttcagaag tcggatgtgg aaatggaggc ccagaaagat
      781 gaaatcatct gccccagctg taataggact gcccatccac tgagacatat taataacgac
      841 atgatagtca ctgacaacaa cggtgcagtc aagtttccac aactgtgtaa attttgtgat
      901 gtgagatttt ccacctgtga caaccagaaa tcctgcatga gcaactgcag catcacctcc
      961 atctgtgaga agccacagga agtctgtgtg gctgtatgga gaaagaatga cgagaacata
     1021 acactagaga cagtttgcca tgaccccaag ctcccctacc atgactttat tctggaagat
     1081 gctgcttctc caaagtgcat tatgaaggaa aaaaaaaagc ctggtgagac tttcttcatg
     1141 tgttcctgta gctctgatga gtgcaatgac aacatcatct tctcagaaga atataacacc
     1201 agcaatcctg acttgttgct agtcatattt caagtgacag gcatcagcct cctgccacca
     1261 ctgggagttg ccatatctgt catcatcatc ttctactgct accgcgttaa ccggcagcag
     1321 aagctgagtt caacctggga aaccggcaag acgcggaagc tcatggagtt cagcgagcac
     1381 tgtgccatca tcctggaaga tgaccgctct gacatcagct ccacgtgtgc caacaacatc
     1441 aaccacaaca cagagctgct gcccattgag ctggacaccc tggtggggaa aggtcgcttt
     1501 gctgaggtct ataaggccaa gctgaagcag aacacttcag agcagtttga gacagtggca
     1561 gtcaagatct ttccctatga ggagtatgcc tcttggaaga cagagaagga catcttctca
     1621 gacatcaatc tgaagcatga gaacatactc cagttcctga cggctgagga gcggaagacg
     1681 gagttgggga aacaatactg gctgatcacc gccttccacg ccaagggcaa cctacaggag
     1741 tacctgacgc ggcatgtcat cagctgggag gacctgcgca agctgggcag ctccctcgcc
     1801 cgggggattg ctaacctcca cagtgatcac actccatgtg ggaggcccaa gatgcccatc
     1861 gtgcacaggg acctcaagag ctccaatatc ctcgtgaaga acgacctaac ctgctgcctg
     1921 tgtgactttg ggctttccct gcgtctggac cctactctgt ctgtggatga cctggctaac
     1981 agtgggcagg tgggaactgc aagatacatg gctccagaag tcctagaatc caggatgaat
     2041 ttggagaatg ttgagtcctt caagcagacc gatgtctact ccatggctct ggtgctctgg
     2101 gaaatgacat ctcgctgtaa tgcagtggga gaagtaaaag attatgagcc tccatttggt
     2161 tccaaggtgc gggagcaccc ctgtgtcgaa agcatgaagg acaacgtgtt gagagatcga
     2221 gggcgaccag aaattcccag cttctggctc aaccaccagg gcatccagat ggtgtgtgag
     2281 acgttgactg agtgctggga ccacgaccca gaggcccgtc tcacagccca gtgtgtggca
     2341 gaacgcttca gtgagctgga gcatctggac aggctctcgg ggaggagctg ctcggaggag
     2401 aagattcctg aagacggctc cctaaacact accaaaagca gcggtggagc aagcgcagcc
     2461 agtggtagcg cggatccacc ggtcgccacc atgagtgcga ttaagccaga catgaagatc
     2521 aaactccgta tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca
     2581 ggcaagcctt ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg
     2641 ccttttgcct ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccaaa
     2701 tatccagaca acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa
     2761 cgaagcttga ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa
     2821 ggggacactt tctataataa agttcgattt tatggtacca actttcccgc caatggtcca
     2881 gttatgcaga agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat
     2941 ggagtgctga cgggtgatat tcatatggct ttgttgcttg aaggaaatgc ccattaccga
     3001 tgtgacttca gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac
     3061 tttgtggacc actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg
     3121 tatgagcatg ctgttgctca ttctggattg cctgacaatg ccagacgata agcggccgcg
     3181 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3241 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3301 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3361 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt
     3421 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3481 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3541 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3601 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3661 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3721 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     3781 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     3841 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     3901 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3961 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     4021 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4081 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4141 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4201 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4261 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4321 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     4381 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4441 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4501 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4561 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     4621 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4681 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4741 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4801 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4861 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4921 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4981 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     5041 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5101 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5161 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5221 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     5281 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5341 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     5401 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     5461 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     5521 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     5581 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     5641 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5701 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5761 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5821 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5881 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5941 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6001 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6061 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6121 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6181 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6241 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6301 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6361 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6421 cctggccttt tgctggcctt ttgctca
//
