LOCUS       sequence-209179-        8603 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcDNA3 - CMVmin - VPR - PIF3, expresses VPR transactivator domain 
            and PIF3, under CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, 
            Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and 
            Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded 
            PhyB Optogenetics.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 
            10.1021/acssynbio.7b00413. Epub 2018 Jan 24.
  PUBMED    29301067
REFERENCE   2  (bases 1 to 8603)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00413"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "706-717"
COMMENT     Sequence Label: pPKm- 227
FEATURES             Location/Qualifiers
     source          1..8603
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             940..1089
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             1114..1134
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1564..1920
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             1939..2508
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     promoter        complement(4145..4163)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4146..4163)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4183..4200)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4189..4413
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4459..4887
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4546..4565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4756..4777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4896..4916)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4901..5230
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5081..5216
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5143..5162
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5297..6091
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5351..5370)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5961..5980
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6265..6386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6302..6321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6356..6375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6435..6451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6435..6451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6448..6470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6459..6475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6483..6489,6490..6507,6508..6513))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6528..6549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6666..6683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6848..7436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6928..6947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7607..8398,8399..8467))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8230..8249
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8468..8572)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccatggaggc tagtggatct gggagagcag atgctctgga tgactttgat
      961 ctggacatgc tgggctccga cgcactcgat gacttcgatc tggatatgct tggcagcgac
     1021 gcactggacg acttcgatct ggacatgttg ggatctgacg cgctggacga cttcgatctg
     1081 gacatgctga tcaattctcg ctcatccggt tcaccaaaga aaaagaggaa ggttgggagt
     1141 cagtatcttc ccgataccga cgatcggcac cggatagagg aaaagaggaa gcggacatac
     1201 gagaccttca aatctatcat gaagaagtca cccttctctg gacccaccga tcccagaccc
     1261 ccgccccggc gcattgccgt accctcccgc agtagcgcct cagtccccaa gcctgcacct
     1321 caaccttatc cttttaccag ttcactgtct accattaatt acgacgagtt tccgacgatg
     1381 gtgtttccct ctggccagat atctcaggcc tctgccctgg cgcctgcacc tccacaggta
     1441 ctgcctcagg caccggcacc cgccccggct cctgcaatgg tgtccgccct cgctcaggcc
     1501 cccgcacctg tgcctgtact tgcccctggc cccccgcagg ctgtcgctcc tcctgccccc
     1561 aagcctaccc aggccgggga gggaactctg tcagaggcct tgttgcagtt gcagttcgat
     1621 gacgaggacc tgggagctct tctgggcaat tctaccgacc ccgccgtctt cactgacctg
     1681 gcaagcgtgg acaatagcga atttcaacag ttgcttaacc agggtattcc ggttgctccg
     1741 catactactg agcccatgct gatggaatac cctgaggcca tcaccagatt ggtcaccggg
     1801 gcacagcggc ctccggaccc agctcccgcc cctctgggcg cccccggact gccaaacggc
     1861 ctgctgtccg gagacgaaga tttctctagt attgccgaca tggacttttc tgcccttttg
     1921 ggcagcggga gcggttccag agatagtcgc gaaggaatgt tcctgccaaa acccgaagca
     1981 ggcagcgcca taagtgacgt gttcgaggga cgggaagtgt gccagccaaa gaggattaga
     2041 ccattccacc caccaggctc tccctgggct aataggcccc tccctgcttc attggcgcct
     2101 actccgactg gtcccgtcca cgagccagtg gggtctctga ctcctgcacc tgtgccacag
     2161 cccctggacc cagccccagc tgtcaccccc gaggccagcc acctgctcga agatccagat
     2221 gaggaaacct cccaggccgt aaaggccttg cgggaaatgg ccgatactgt aatcccacag
     2281 aaagaggagg ccgccatttg tgggcaaatg gatctcagtc atccccctcc aaggggtcac
     2341 ctggatgaat tgaccacaac cttggaatca atgacagaag acctcaatct ggatagcccc
     2401 ctgacgccgg agctgaacga gattcttgac acttttctca acgatgaatg cctcttgcat
     2461 gccatgcata tcagcaccgg cctgtctatt tttgatacct ccttgtttga cagcgccggc
     2521 agcgccggca gcgccggcag cgaattcatg cctctgtttg agcttttcag gctcaccaaa
     2581 gctaagcttg aatctgctca agacaggaac ccttctccac ctgtagatga agttgtggag
     2641 ctggtgtggg aaaatggtca gatatcaact caaagtcagt caagtagatc gaggaacatt
     2701 cctccaccac aagcaaactc ttctagagct agagagattg gaaatggctc aaagacgact
     2761 atggtggacg agatccctat gtcagtgcca tcactaatga cgggtttgag tcaagacgat
     2821 gactttgttc catggttgaa tcatcatccc tcccttgatg gatattgctc tgatttcttg
     2881 cgtgatgtgt cgtctcctgt tactgtcaac gagcaagaga gtgatatggc ggtaaaccaa
     2941 actgctttcc cgttgtttca gagaagaaag gatggcaatg aatcagctcc tgctgcttct
     3001 tcgtcgcagt ataacggttt ccaatcgcat tctctgtatg gaagtgatag agctagagat
     3061 cttcctagcc aacaaaccaa tccggatcgg tttactcaga cgcaggaacc actaattact
     3121 agtaacaagc ctagtttggt caacttttca catttcttac gccctgcaac ttttgcgaag
     3181 actactaata ataaccttca tgacactaaa gaaaagagtc ctcaaagccc gccaaatgtg
     3241 tttcagacca gagttcttgg agctaaagac tctgaagata aggttcttaa cgagtctgtt
     3301 gcttctgcta cgcctaaaga taaccaaaag gcttgcctaa tatcagagga ctcatgtaga
     3361 aaagaccaag agagtgaaaa agcagttgta tgttcttctg ttggctcggg taatagtctc
     3421 gatggcccat ccgaaagtcc ttcactttct ttaaagagaa agcattcgaa tattcaagac
     3481 attgactgtc atagtgaaga tgtggaagaa gaatcaggag atggaagaaa ggaagcaggt
     3541 ccatctcgaa cgggtttggg ttcaaagaga agccgctctg cagaagtgca taatctgtct
     3601 gaaaggagac ggcgtgatag gatcaacgag aagatgcgtg ccctgcaaga actcattcca
     3661 aactgtaaca aggtggacaa agcttcgatg ctagatgaag ccatcgagta tctcaagtca
     3721 ctccaacttc aagtgcagat catgtcaatg gcgtctggtt actatctgcc accggcggtt
     3781 atgttcccac cgggtatggg gcattacccg gcagcagctg ctgcaatggc aatgggtatg
     3841 ggaatgcctt atgcaatggg cttgcctgat ttgagccgtg gtggttcatc ggttaaccac
     3901 ggaccacagt tccaagtctc ggggatgcaa caacaaccag tggcgatggg tattccacgt
     3961 gtctctggtg gtggtatctt tgccggttct tcgacgattg gcaatggctc gactagagat
     4021 ttatctggtt ctaaagatca aacaacgacg aataacaaca gtaacttgaa accaataaag
     4081 agaaaacagg ggtcttctga tcagttttgt ggatcgtcgt aagcatgcat ctagagggcc
     4141 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
     4201 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4261 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4321 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4381 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4441 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4501 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4561 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4621 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4681 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4741 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4801 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4861 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     4921 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4981 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5041 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5101 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5161 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5221 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     5281 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5341 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5401 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5461 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5521 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5581 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5641 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5701 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5761 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     5821 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     5881 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     5941 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6001 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6061 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     6121 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6181 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6241 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6301 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6361 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     6421 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6481 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6541 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6601 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6781 tgagcaaaag gccagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8221 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8581 cccgaaaagt gccacctgac gtc
//
