LOCUS       sequence-209195-       13287 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13287)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETgc-2in1-CC
FEATURES             Location/Qualifiers
     source          1..13287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        165..509
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    603..727
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(752..757,758..775,776..782)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             836..1495
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(902..921)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1837..2142
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2021..2040
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2183..2307)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2327..3037
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2740..2759
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        3417..3761
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    3856..3979
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4014..4019,4020..4037,4038..4044)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4052..4068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4057..4079
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4076..4092
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4076..4092
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4353..4477)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(4497..4499,4500..4502,4503..5219)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKL"
     primer_bind     complement(4542..4563)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4803..4822)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5150..5171
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5539..5717
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6330..6582
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6687..6711
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7232..8023
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8269..8857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8758..8777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9011..9028
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9043..9183
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9109..9128)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9269..9291
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9527..9721
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9787..10860)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11289..11918)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13249..13273
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagcaacg
       61 acggccagtg aattcaggcc tctgcaggtc aacatggtgg agcacgacac tctcgtctac
      121 tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa
      181 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
      241 aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct
      301 atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac
      361 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
      421 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
      481 taaggaagtt catttcattt ggagaggacc tcgagcgcgc gtatttttaa acaattacca
      541 acaacaacaa caaacaacaa caacattaca ttttacattc tacaactaca tctagtccta
      601 gaacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa tgatataaat atcaatatat
      661 taaattagat tttgcataaa aaacagacta cataatactg taaaacacaa catatccagt
      721 cactatggcg gccgcattag gcaccccagg ctttacactt tatgcttccg gctcgtataa
      781 tgtgtggatt ttgagttagg atccgtcgag attttcagga gctaaggaag ctaaaatgga
      841 gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt
      901 tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac
      961 ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat
     1021 tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct
     1081 ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt
     1141 ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca
     1201 agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat
     1261 gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa
     1321 tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa
     1381 ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg
     1441 cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaaagatc
     1501 tggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     1561 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     1621 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     1681 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     1741 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     1801 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     1861 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     1921 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
     1981 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     2041 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     2101 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     2161 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
     2221 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     2281 tcgttcaact tttctataca aagttggtca attgttaggc actagtatgg tgagcaaggg
     2341 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     2401 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     2461 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     2521 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     2581 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     2641 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     2701 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     2761 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     2821 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     2881 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     2941 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     3001 ccactccacc ggcggcatgg acgagctgta caagtaatta taagcggcca tgctagagtc
     3061 cgcaaaaatc accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat
     3121 gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga
     3181 gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt
     3241 ctaattccta aaaccaaaat ccagtgacct aggaggcctg catgcaagct tggcgtaatc
     3301 atggtcgctc gcacgtgcgg agctccgtac cctctgcagg tcaacatggt ggagcacgac
     3361 actctcgtct actccaagaa tatcaaagat acagtctcag aagaccagag ggctattgag
     3421 acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt
     3481 cacttcatcg aaaggacagt agaaaaggaa gatggcttct acaaatgcca tcattgcgat
     3541 aaaggaaagg ctatcgttca agatgcctct accgacagtg gtcccaaaga tggaccccca
     3601 cccacgagga acatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
     3661 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
     3721 ccttcctcta tataaggaag ttcatttcat ttggagagga cctcgagcgc gcgtattttt
     3781 aaacaattac caacaacaac aacaaacaac aacaacatta cattttacat tctacaacta
     3841 catctagtca attggcaact ttgtataata aagttgaacg agaaacgtaa aatgatataa
     3901 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac
     3961 aacatatcca gtcactatgg cggccgcggg ctcactcatt aggcacccca ggctttacac
     4021 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     4081 aacagctatg accatgatta ccccgtccct gcacgcctgt cgttcaaccc tggaagatcc
     4141 gcgtgtcccg agttctaaca gtctggccgt cgttctgcag cgtcgcgatt gggaaaaccc
     4201 gggcgtgacc caactgaatc gtctggcggc ccatccgccg tttgcgtcgt ggcgtaactc
     4261 agaagaagcc cgtaccgacc gtccgtctca acaactgcgc tcctaacact ataaaaagct
     4321 gatgcagacg gggctttttt ctgcaggtcg accatagtga ctggatatgt tgtgttttac
     4381 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
     4441 tttacgtttc tcgttcagct ttcttgtaca aagtggtgtc taggttaggc actagtatgg
     4501 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4561 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4621 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4681 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4741 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4801 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4861 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4921 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4981 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     5041 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     5101 tgagtactca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     5161 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagttataag
     5221 cggccatgct agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat
     5281 ttttctccag aataatgtgt gagtagttcc cagataaggg aattagggtt cttatagggt
     5341 ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt gtaaaatact
     5401 tctatcaata aaatttctaa ttcctaaaac caaaatccag tgacctagga gggtagatca
     5461 tgagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg acaagccgtt
     5521 ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actgagcgcg ggtttctgga
     5581 gtttaatgag ctaagcacat acgtcagaaa ccattattgc gcgttcaaaa gtcgcctaag
     5641 gtcactatca tctagcaaat atttcttgtc aaaaatgctc cactgacgtt ccataaattc
     5701 ccctcggtat ccaattagag tctcatattc actctcaact cgatcgaggg gatctaccat
     5761 gagtccagaa aggagaccag cagatattag gagagcaacc gaagcagata tgccagcagt
     5821 ttgcaccatt gtgaaccatt acatcgagac ttctacagtt aattttagga ctgaacctca
     5881 agagccacag gaatggacag atgatctcgt gagattaagg gaaagatacc cttggcttgt
     5941 tgctgaggtg gatggagaag ttgcaggtat tgcttacgca ggaccttgga aggctagaaa
     6001 cgcttatgat tggactgctg agtctaccgt ttacgtgtca cctagacatc aaagaaccgg
     6061 acttggttca accttgtata ctcacctttt gaagtctctt gaagctcagg gattcaaatc
     6121 tgttgtggct gttatcggtt tgcctaatga tccaagtgtg agaatgcatg aagctctcgg
     6181 atacgcacca aggggtatgt taagagctgc tggatttaaa catggtaact ggcacgatgt
     6241 tggtttctgg cagttagatt tcagtttacc agttccacca agaccagtgc ttccagtgac
     6301 cgagatttga cccgggctag agtcaagcag atcgttcaaa catttggcaa taaagtttct
     6361 taatattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     6421 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     6481 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     6541 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgaccggca tgaagctgag
     6601 ttaacgatgt aagtcctcaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg
     6661 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc
     6721 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca
     6781 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg
     6841 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga
     6901 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg
     6961 aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc
     7021 acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt
     7081 catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     7141 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     7201 gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc
     7261 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     7321 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     7381 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     7441 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     7501 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     7561 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     7621 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     7681 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     7741 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     7801 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     7861 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     7921 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     7981 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc
     8041 gttcaagccg acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca
     8101 taattgctca cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata
     8161 ataagcccta cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt
     8221 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8281 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8341 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8401 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8461 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8521 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8581 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8641 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8701 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8761 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8821 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8881 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8941 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9001 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     9061 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     9121 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     9181 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     9241 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     9301 tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga
     9361 cggcgcggct tgtccgcgcc ctggtagatt gcctggccct aggccagcca tttttgagcg
     9421 gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag
     9481 ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag
     9541 gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt
     9601 ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc
     9661 aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac
     9721 aggaaagaga ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc
     9781 cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag
     9841 gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc
     9901 gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc
     9961 gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg
    10021 gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac
    10081 gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat
    10141 gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc
    10201 ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg
    10261 cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc
    10321 tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc
    10381 tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac
    10441 caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc
    10501 gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa
    10561 aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac
    10621 gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc
    10681 cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg
    10741 gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac
    10801 caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac
    10861 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg
    10921 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
    10981 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
    11041 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
    11101 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
    11161 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
    11221 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
    11281 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
    11341 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
    11401 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
    11461 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
    11521 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
    11581 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
    11641 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
    11701 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
    11761 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
    11821 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
    11881 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
    11941 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
    12001 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
    12061 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt
    12121 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
    12181 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
    12241 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
    12301 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
    12361 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
    12421 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
    12481 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
    12541 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
    12601 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
    12661 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
    12721 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
    12781 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
    12841 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
    12901 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
    12961 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
    13021 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
    13081 gcggaggggt tggatcaaag tactttaaag tactttaaag tactttaaag tactttgatc
    13141 ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca
    13201 cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt ttacccgcca
    13261 atatatcctg tcaaacactg atagttt
//
