LOCUS       sequence-209202-        5409 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Key genes: ""mini-CcaS #10"", ho1, pcyA, spectinomycin resistance, 
            and medium copy backbone (p15a).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ong NTX, Tabor JJ
  TITLE     A miniaturized E. coli green light sensor with high dynamic range.
  JOURNAL   Chembiochem. 2018 Feb 8. doi: 10.1002/cbic.201800007.
  PUBMED    29420866
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2018 Feb 8. doi: 10.1002/cbic.201800007."
COMMENT     Sequence Label: pNO286-3
FEATURES             Location/Qualifiers
     source          1..5409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      321..415
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      529..1074
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     975..994
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1191..1208
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1330..1364
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     terminator      2809..2880
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2896..2923
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        3040..3074
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     RBS             3091..3102
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             3834..3845
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      4613..4684
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4700..4727
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             join(4927..5409,1..309)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
       61 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      121 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
      181 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
      241 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
      301 ggcaaataag agctcgcttg gactcctgtt gatagatcca gtaatgacct cagaactcca
      361 tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag
      421 cactagtaac aacttatatc gtatggggct gacttcaggt gctacatttg aagagataaa
      481 ttgcactgaa atctagaaat attttatctg attaataaga tgatcttctt gagatcgttt
      541 tggtctgcgc gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc
      601 gaaggttctc tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc
      661 accaaaactt gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc
      721 taaatcaatt accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact
      781 caagacgata gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac
      841 agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa
      901 cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg
      961 agggagccgc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac
     1021 tgatttgagc gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc
     1081 tttgccgcgg ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc
     1141 cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag
     1201 tgagcgagga agcggaatat atcccctagg tggagtctgg cctcaaatac aaagtctgca
     1261 aagtctcgaa atattaaacc agagaataaa taattaaacg ccaaaatatt tctagcaaaa
     1321 accccagttt ttacggctag ctcagtccta ggtatagtgc tagctataga ggttaaaatc
     1381 ttaataggag gaaggcgcaa tgggcaaatt tctaattcca atcgaatttg tttttctggc
     1441 gatcgccatg acctgttatt tatggcacag acaaaaccaa gaacgccgca ggattgaaat
     1501 tagcatcaag caacaaaccc aacgggaacg atttattaac caaattaccc aacatatccg
     1561 ccaatcttta aacttggaaa cggttttaaa taccaccgtc gctgaagtta aaaccctgtt
     1621 gcaagttgat cgagttctaa tttatcgcat ttggcaagat ggcacgggca gcgccattac
     1681 ggaatcggtg aatgccaatt atcctagtat tttagggcgg accttttccg atgaagtttt
     1741 tcccgttgaa taccatcaag cctacaccaa aggtaaagta cgggccatta atgacattga
     1801 ccaggatgac atagagattt gcctagctga tttcgtcaaa caatttggcg tgaaatcaaa
     1861 attagtagtg cccattcttc aacataatcg tgcttcttcc ctagataatg aatcagaatt
     1921 tccctatctt tgggggctgt taattaccca tcaatgtgct tttacccggc catggcaacc
     1981 gtgggaagtg gagttaatga aacagctagc caatcaggtc gcgatcgcca tccaacaatc
     2041 ggaattatat gagcaattgc agctagcttt agaacgggaa aaagaattaa gccgcctaaa
     2101 aactcgtttt ttctccatgg cttcccatga atttcgtact cccctcagta cggccttagc
     2161 tgctgcccaa ttactggaaa attctgaagt ggcctggctt gatcccgata agcgtagccg
     2221 gaacttacac cgtattcaaa attccgtgaa aaatatggta cagctcctgg atgatatttt
     2281 aatcattaac cgtgccgaag cgggcaaatt ggaatttaat cctaattggt tagatttgaa
     2341 attattgttc cagcaattta tcgaagaaat tcaattaagt gtcagtgacc aatattattt
     2401 tgactttatt tgtagcgctc aagatacgaa ggcattggtg gatgaaaggt tagtgcggtc
     2461 tattttatct aatctgttat ctaatgcgat taaatactct cccgggggag ggcagattaa
     2521 aattgcccta agcctagatt cggaacagat tatttttgaa gtcaccgacc agggcattgg
     2581 catttcgcca gaggaccaaa agcaaatttt tgaacccttt catcggggca aaaatgtcag
     2641 aaatattacg ggaacaggac tcggtttaat ggttgccaag aaatgtgttg acttacacag
     2701 tggcagtatc ttgctaaaaa gtgcagttga ccagggaaca acagttacta tctgtttaaa
     2761 acgctataac catttgcctc gagcttagta ataatctaga ccaggcatca aataaaacga
     2821 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2881 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatag taaatcactg
     2941 cataattcgt gtcgattaaa ccgcctaagt cccccaggaa agggggatat aacagtatag
     3001 attttgtcag ccttcagctt ggctttaccg tcaaaaaaac tgacagctag ctcagtccta
     3061 ggtataatgc tagctcacac agaattcatt aaagaggaga aaggtaccat gagtgtcaac
     3121 ttagcttccc agttgcggga agggacgaaa aaatcccact ccatggcgga gaacgtcggc
     3181 tttgtcaaat gcttcctcaa gggcgttgtc gagaaaaatt cctaccgtaa gctggttggc
     3241 aatctctact ttgtctacag tgccatggaa gaggaaatgg caaaatttaa ggaccatccc
     3301 atcctcagcc acatttactt ccccgaactc aaccgcaaac aaagcctaga gcaagacctg
     3361 caattctatt acggctccaa ctggcggcaa gaagtgaaaa tttctgccgc tggccaagcc
     3421 tatgtggacc gagtccggca agtggccgct acggcccctg aattgttggt ggcccattcc
     3481 tacacccgtt acctggggga tctttccggc ggtcaaattc tcaagaaaat tgcccaaaat
     3541 gccatgaatc tccacgatgg tggcacagct ttctatgaat ttgccgacat tgatgacgaa
     3601 aaggctttta aaaataccta ccgtcaagct atgaatgatc tgcccattga ccaagccacc
     3661 gccgaacgga ttgtggatga agccaatgac gcctttgcca tgaacatgaa aatgttcaac
     3721 gaacttgaag gcaacctgat caaggcgatc ggcattatgg tgttcaacag cctcacccgt
     3781 cgccgcagtc aaggcagcac cgaagttggc ctcgccacct ccgaaggcta gttaaagagg
     3841 agaaaggatc catggccgtc actgatttaa gtttgaccaa ttcttccctg atgcccacgt
     3901 tgaacccgat gattcaacag ttggccctgg cgatcgccgc tagttggcaa agtttacccc
     3961 tcaagcccta tcaattgccg gaggatttgg gctacgtaga aggccgcctg gaaggggaaa
     4021 agttagtgat tgaaaatcgg tgctaccaaa cgccccagtt tcgcaaaatg catttggagt
     4081 tggccaaggt gggcaaaggg ttggatattc tccactgtgt aatgtttcct gagcctttat
     4141 acggtctacc tttgtttggc tgtgacattg tggccggccc cggtggagta agtgcggcta
     4201 ttgcggatct atcccccacc caaagcgatc gccaattgcc cgcagcgtac caaaaatcat
     4261 tggcagagct aggccagcca gaatttgagc aacaacggga attgcccccc tggggagaaa
     4321 tattttctga atattgttta ttcatccgtc ccagcaatgt cactgaagaa gaaagatttg
     4381 tacaaagggt agtggacttt ttgcaaattc attgtcacca atccatcgtt gccgaaccct
     4441 tgtctgaagc tcaaactttg gagcaccgtc aggggcaaat tcattactgc caacaacaac
     4501 agaaaaatga taaaacccgt cgggtactgg aaaaagcttt tggggaagct tgggcggaac
     4561 ggtatatgag ccaagtctta tttgatgtta tccaataatc tagaccaggc atcaaataaa
     4621 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     4681 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ataggtaccc
     4741 ggggatcctc tagagtcgac ctgcaggcat gcaagcttag caagcgaacc ggaattgcca
     4801 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
     4861 ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg
     4921 tttcgcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     4981 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     5041 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     5101 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     5161 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     5221 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     5281 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     5341 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     5401 gaacaggat
//
