LOCUS       sequence-209207-       14615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of mRIN2 dRA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14615)
  AUTHORS   Sandri C, Caccavari F, Valdembri D, Camillo C, Veltel S, 
            Santambrogio M, Lanzetti L, Bussolino F, Ivaska J, Serini G
  TITLE     The R-Ras/RIN2/Rab5 complex controls endothelial cell adhesion and 
            morphogenesis via active integrin endocytosis and Rac signaling.
  JOURNAL   Cell Res. 2012 Oct;22(10):1479-501. doi: 10.1038/cr.2012.110. Epub 
            2012 Jul 24.
  PUBMED    22825554
REFERENCE   2  (bases 1 to 14615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2012.110"; journalName: "Cell Res"; date: "2012-10"; 
            volume: "22"; issue: "10"; pages: "1479-501"
COMMENT     Sequence Label: HA-mRIN2 dRA/PINCO
FEATURES             Location/Qualifiers
     source          1..14615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             332..801
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    866..1223
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1288..1704
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1634..1656
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1672..1688
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1714..2088
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2026..2042
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             2100..2126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2106..2132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     enhancer        4427..4806
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4807..5010
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4961..4981
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5055..5074
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5055..5073
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5105..5114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5111..5113,5114..5116,5117..5830)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5111..5113,5114..5116,5117..5827)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5156..5177)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5417..5436)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5764..5785
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6020..6489
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6619..6636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6790..7378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6870..6889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7501..7635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7525..7544)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7579..7598
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7865..8464)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7948..7968)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8445..8464
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8547..9046)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8595..8614)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     8988..9011
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      11162..12951
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(13445..14236,14237..14305))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14068..14087
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14306..14410)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgcaatattc ctgttaccaa agctagtata aataaaaata gataaacgtg gaaattactt
       61 agagtttctg tcattaacgt ttccttcctc agttgacaac ataaatgcgc tgctgagaag
      121 ccagtttgca tctgtcagga tcaatttccc attatgccag tcatattaat tactagtcaa
      181 ttagttgatt tttatttttg acatatacat gtgaaagacc ccacctgtag gtttggcaag
      241 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagaaaag
      301 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
      361 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
      421 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
      481 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
      541 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
      601 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggcgc
      661 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
      721 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
      781 cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg
      841 gaccaccgac ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt
      901 gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta ctagttagct aactagctct
      961 gtatctggcg gacccgtggt ggaactgacg agttcggaac acccggccgc aaccctggga
     1021 gacgtcccag ggacttcggg ggccgttttt gtggcccgac ctgagtccaa aaatcccgat
     1081 cgttttggac tctttggtgc acccccctta gaggagggat atgtggttct ggtaggagac
     1141 gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg ggaccgaagc
     1201 cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt
     1261 ctgtatttgt ctgaaaatat gggcccgggc cagactgtta ccactccctt aagtttgacc
     1321 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
     1381 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
     1441 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
     1501 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
     1561 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
     1621 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
     1681 gccctcactc cttctctagg cgcccccata tggccatatg agatcttata tggggcaccc
     1741 ccgccccttg taaacttccc tgaccctgac atgacaagag ttactaacag cccctctctc
     1801 caagctcact tacaggctct ctacttagtc cagcacgaag tctggagacc tctggcggca
     1861 gcctaccaag aacaactgga ccgaccggtg gtacctcacc cttaccgagt cggcgacaca
     1921 gtgtgggtcc gccgacacca gactaagaac ctagaacctc gctggaaagg accttacaca
     1981 gtcctgctga ccacccccac cgccctcaaa gtagacggca tcgcagcttg gatacacgcc
     2041 gcccacgtga aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatccatgt
     2101 acccatacga tgttccggat tacgctagcc tcccagatct cgacccattt tgcattctca
     2161 gcatggtgag acacaaggat ggtggctact ctgaagacaa ggatgggaag acatgtcccc
     2221 gagactctgg ctatgacagc ctctccaaca ggctcagcat tctggaccgg ctcctgcata
     2281 cccatcccat atggctgcag ctgagtctga gtgaggagga ggcagcagag gtcctgcagg
     2341 cccagcctcc agggatcttt ttggttcgga aatcctccaa aatgcagaag aaagtcctct
     2401 cccttcgcct gccctgtgaa tttggagcac ctctcaaaga gtttactata aaggaaagca
     2461 cctacacctt ttccctggaa ggctcaggaa tcagctttgc agatttattc cggcttattg
     2521 ccttctactg catcagcagg gacgttttgc cattcacctt gaagctgcct tatgccattt
     2581 caacagccaa gacggagtct cagcttgaag aactagccca gctgggacta aatttttgga
     2641 gctcctcagc agacaacaaa cccccgaact ccccacctcc ccataggcct cttccctcag
     2701 ccggcatctg ccctgcctcc ttgcgtcagc tctgccttat aaatggagtg cattctataa
     2761 aaaccaggac gccttcggag ctggagtgca gccagaccaa cggagccttg tgttttatta
     2821 atcctctttt cttgaaagtg cacagccagg acctcagcac aggcccaaag cggcccagca
     2881 caaggactcc caacgcgaat ggcaccgaga gacctcggtc ccccccaccc aggcccccgc
     2941 cacccgctat taatagtctg cacacaagcc ctgggctatc caggactgaa ccccagacga
     3001 gcatgccaga aacagtcaac catagcaaac atgggaacgt agctctgctt ggaacaaaac
     3061 caactcccat ccctccaccc cggctgaaga agcaggcttc ttttttggaa gcagaaagca
     3121 gcgccaagac cttgactgcc cgccggccca gccggagctc ggagccggag cccgagctag
     3181 agctggagtt agagatgggc acagctgggc acgcaggtgg agccccgcct agggacgccc
     3241 ccggggattg cacaagggcg ccgccgcccg gctctgaatc acaaccacca ccgtgccatg
     3301 gagccaggca gaggctgagc gacatgagcc tttccacttc ctcctccgac tcgctggagt
     3361 tcgaccggag catgcctctg tatgggtacg aggccgacac caccagcagc ctggaggact
     3421 acgaagggga gagtgaccaa gagaccatgg cacctcccat caagtccaaa aagaagagga
     3481 acagctcttt tgtgctgccc aagcttgtca agtcccagtt gcgaaagatg agcggggtgt
     3541 tcagctcctt catgacccct gagaagcgga tggtccgcag gatcgccgag ctgtcccggg
     3601 acaaatgcac ctattttggg tgcttggtgc aggactacgt gagcttcctg aaggagaaca
     3661 aggagtgcca tgtgtccagc accgatatgc tgcagaccat ccggcagttt atgacccagg
     3721 tcaagaacta tttgtctcag agctctgaac tggacccccc tattgagtca ctgatcccag
     3781 aagaccaaat tgatgtggtg ttggagaagg ccatgcacaa atgtatcttg aagcccctca
     3841 agggccatgt ggaggccatg ctgaaggact tccacacggc tgacggctcg tggaaacaac
     3901 tcaaggaaaa cctacaactc gtgcggcaga ggaacccaca ggagctgggg gtctttgccc
     3961 caacccctga cttgatggag ctggagaaaa tcaagcttaa attcatgacc atgcagaaga
     4021 tgtattcgcc agaaaagaaa gtcatgcttt tgctacgggt ctgtaagctc atctacactg
     4081 tcatggagaa caactcaggg aggatgtacg gtgcagacga cttcttgcca gtcctgacct
     4141 atgtcatagc tcagtgtgac atgcttgaac tggacacgga aatcgagtac atgatggaac
     4201 tcctggaccc atcactgctg cacggagaag gaggctatta cttgaccagt gcctatgggg
     4261 cgctatctct gataaagaat ttccaagaag aacaggctgc gagactgctc agctccgaag
     4321 ccagggacac gctgaggcag tggcacaagc ggagaaccac caaccgaact atcccctccg
     4381 ttgacgacta ggaattcgat cgatgtacgg gccagatata cgcgttgaca ttgattattg
     4441 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     4501 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     4561 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     4621 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     4681 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     4741 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     4801 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     4861 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     4921 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     4981 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
     5041 tggcttatcg aaattaatac gactcactat agggagaccc aagcttcgaa ttgatccacc
     5101 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     5161 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     5221 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     5281 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     5341 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     5401 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     5461 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     5521 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     5581 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     5641 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     5701 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     5761 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     5821 gtacaagtaa agcggccgcc agcacagtgg tcgacgataa aataaaagat tttatttagt
     5881 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     5941 acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg
     6001 tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc
     6061 tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct
     6121 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
     6181 agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
     6241 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct
     6301 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg
     6361 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
     6421 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
     6481 ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat
     6541 taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6601 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6661 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6721 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     6781 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6841 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6901 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6961 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7021 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7081 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7141 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7201 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7261 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7321 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7381 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7441 gattttggtc atgagattat caaaaaggat cttcacctag atccttttgc ggccgaatta
     7501 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     7561 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     7621 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     7681 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     7741 cctctagagt cggtgggcct cgggggcggg tgcggggtcg gcggggccgc cccgggtggc
     7801 ttcggtcgga gccatggggt cgtgcgctcc tttcggtcgg gcgctgcggg tcgtggggcg
     7861 ggcgtcaggc accgggcttg cgggtcatgc accaggtgcg cggtccttcg ggcacctcga
     7921 cgtcggcggt gacggtgaag ccgagccgct cgtagaaggg gaggttgcgg ggcgcggagg
     7981 tctccaggaa ggcgggcacc ccggcgcgct cggccgcctc cactccgggg agcacgacgg
     8041 cgctgcccag acccttgccc tggtggtcgg gcgagacgcc gacggtggcc aggaaccacg
     8101 cgggctcctt gggccggtgc ggcgccagga ggccttccat ctgttgctgc gcggccagcc
     8161 gggaaccgct caactcggcc atgcgcgggc cgatctcggc gaacaccgcc cccgcttcga
     8221 cgctctccgg cgtggtccag accgccaccg cggcgccgtc gtccgcgacc cacaccttgc
     8281 cgatgtcgag cccgacgcgc gtgaggaaga gttcttgcag ctcggtgacc cgctcgatgt
     8341 ggcggtccgg gtcgacggtg tggcgcgtgg cggggtagtc ggcgaacgcg gcggcgaggg
     8401 tgcgtacggc ccgggggacg tcgtcgcggg tggcgaggcg caccgtgggc ttgtactcgg
     8461 tcatggaagg tcgtctcctt gtgaggggtc aggggcgtgg gtcaggggat ggtggcggca
     8521 ccggtcgtgg cggccgacct gcaggtcgaa aggcccggag atgaggaaga ggagaacagc
     8581 gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg
     8641 cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct tcccagcctc
     8701 tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag gcctacccgc
     8761 ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc tacttccatt
     8821 tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac taggggagga
     8881 gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct accggtggat
     8941 gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag cggggctgct
     9001 aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta gaattaattc
     9061 tcatgtttga cagcttatca tcgataagct gatcctcaca ggccgcaccc agcttttctt
     9121 ccgttgcccc agtagcatct ctgtctggtg accttgaaga ggaagaggag gggtcccgag
     9181 aatccccatc cctaccgtcc agcaaaaagg gggacgagga atttgaggcc tggcttgagg
     9241 ctcaggacgc aaatcttgag gatgttcagc gggagttttc cgggctgcga gtaattggtg
     9301 atgaggacga ggatggttcg gaggatgggg aattttcaga cctggatctg tctgacagcg
     9361 accatgaagg ggatgagggt gggggggctg ttggaggggg caggagtctg cactccctgt
     9421 attcactgag cgtcgtctaa taaagatgtc tattgatctc ttttagtgtg aatcatgtct
     9481 gacgaggggc caggtacagg acctggaaat ggcctaggag agaagggaga cacatctgga
     9541 ccagaaggct ccggcggcag tggacctcaa agaagagggg gtgataacca tggacgagga
     9601 cggggaagag gacgaggacg aggaggcgga agaccaggag ccccgggcgg ctcaggatca
     9661 gggccaagac atagagatgg tgtccggaga ccccaaaaac gtccaagttg cattggctgc
     9721 aaagggaccc acggtggaac aggagcagga gcaggagcgg gaggggcagg agcaggaggg
     9781 gcaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg agcaggaggg
     9841 gcaggrgcag gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggggca
     9901 ggagcaggag gtggaggccg gggtcgagga ggcagtggag gccggggtcg aggaggtagt
     9961 ggaggccggg gtcgaggagg tagtggaggc cgccggggta gaggacgtga aagagccagg
    10021 gggggaagtc gtgaaagagc cagggggaga ggtcgtggac gtggagaaaa gaggcccagg
    10081 agtcccagta gtcagtcatc atcatccggg tctccaccgc gcaggccccc tccaggtaga
    10141 aggccatttt tccaccctgt aggggaagcc gattattttg aataccacca agaaggtggc
    10201 ccagatggtg agcctgacgt gcccccggga gcgatagagc agggccccgc agatgaccca
    10261 ggagaaggcc caagcactgg accccggggt cagggtgatg gaggcaggcg caaaaaagga
    10321 gggtggtttg gaaagcatcg tggtcaagga ggttccaacc cgaaatttga gaacattgca
    10381 gaaggtttaa gagctctcct ggctaggagt cacgtagaaa ggactaccga cgaaggaact
    10441 tgggtcgccg gtgtgttcgt atatggaggt agtaagacct ccctttacaa cctaaggcga
    10501 ggaactgccc ttgctattcc acaatgtcgt cttacaccat tgagtcgtct cccctttgga
    10561 atggcccctg gacccggccc acaacctggc ccgctaaggg agtccattgt ctgttatttc
    10621 atggtctttt tacaaactca tatatttgct gaggttttga aggatgcgat taaggacctt
    10681 gttatgacaa agcccgctcc tacctgcaat atcagggtga ctgtgtgcag ctttgacgat
    10741 ggagtagatt tgcctccctg gtttccacct atggtggaag gggctgccgc ggagggtgat
    10801 gacggagatg acggagatga aggaggtgat ggagatgagg gtgaggaagg gcaggagtga
    10861 tgtaacttgt taggagacgc cctcaatcgt attaaaagcc gtgtattccc ccgcactaaa
    10921 gaataaatcc ccagtagaca tcatgcgtgc tgttggtgta tttctggcca tctgtcttgt
    10981 caccattttc gtcctcccaa catggggcaa ttgggcatac ccatgttgtc acgtcactca
    11041 gctccgcgct caacaccttc tcgcgttgga aaacattagc gacatttacc tggtgagcaa
    11101 tcagacatgc gacggcttta gcctggcctc cttaaattca cctaagaatg ggagcaacca
    11161 gcaggaaaag gacaagcagc gaaaattcac gcccccttgg gaggtggcgg catatgcaaa
    11221 ggatagcact cccactctac tactgggtat catatgctga ctgtatatgc atgaggatag
    11281 catatgctac ccggatacag attaggatag catatactac ccagatatag attaggatag
    11341 catatgctac ccagatatag attaggatag cctatgctac ccagatataa attaggatag
    11401 catatactac ccagatatag attaggatag catatgctac ccagatatag attaggatag
    11461 cctatgctac ccagatatag attaggatag catatgctac ccagatatag attaggatag
    11521 catatgctat ccagatattt gggtagtata tgctacccag atataaatta ggatagcata
    11581 tactacccta atctctatta ggatagcata tgctacccgg atacagatta ggatagcata
    11641 tactacccag atatagatta ggatagcata tgctacccag atatagatta ggatagccta
    11701 tgctacccag atataaatta ggatagcata tactacccag atatagatta ggatagcata
    11761 tgctacccag atatagatta ggatagccta tgctacccag atatagatta ggatagcata
    11821 tgctatccag atatttgggt agtatatgct acccatggca acattagccc accgtgctct
    11881 cagcgacctc gtgaatatga ggaccaacaa ccctgtgctt ggcgctcagg cgcaagtgtg
    11941 tgtaatttgt cctccagatc gcagcaatcg cgcccctatc ttggcccgcc cacctactta
    12001 tgcaggtatt ccccggggtg ccattagtgg ttttgtgggc aagtggtttg accgcagtgg
    12061 ttagcggggt tacaatcagc caagttatta cacccttatt ttacagtcca aaaccgcagg
    12121 gcggcgtgtg ggggctgacg cgtgccccca ctccacaatt tcaaaaaaaa gagtggccac
    12181 ttgtctttgt ttatgggccc cattggcgtg gagccccgtt taattttcgg gggtgttaga
    12241 gacaaccagt ggagtccgct gctgtcggcg tccactctct ttccccttgt tacaaataga
    12301 gtgtaacaac atggttcacc tgtcttggtc cctgcctggg acacatctta ataaccccag
    12361 tatcatattg cactaggatt atgtgttgcc catagccata aattcgtgtg agatggacat
    12421 ccagtcttta cggcttgtcc ccaccccatg gatttctatt gttaaagata ttcagaatgt
    12481 ttcattccta cactagtatt tattgcccaa ggggtttgtg agggttatat tggtgtcata
    12541 gcacaatgcc accactgaac cccccgtcca aattttattc tgggggcgtc acctgaaacc
    12601 ttgttttcga gcacctcaca tacaccttac tgttcacaac tcagcagtta ttctattagc
    12661 taaacgaagg agaatgaaga agcaggcgaa gattcaggag agttcactgc ccgctccttg
    12721 atcttcagcc actgcccttg tgactaaaat ggttcactac cctcgtggaa tcctgacccc
    12781 atgtaaataa aaccgtgaca gctcatgggg tgggagatat cgctgttcct taggaccctt
    12841 ttactaaccc taattcgata gcatatgctt cccgttgggt aacatatgct attgaattag
    12901 ggttagtctg gatagtatat actactaccc gggaagcata tgctacccgt ttagggttaa
    12961 caagggggcc ttataaacac tattgctaat gccctcttga gggtccgctt atcggtagct
    13021 acacaggccc ctctgattga cgttggtgta gcctcccgta gtcttcctgg gcccctggga
    13081 ggtacatgtc ccccagcatt ggtgtaagag cttcagccaa gagttacaca taaaggcaat
    13141 gttgtgttgc agtccacaga ctgcaaagtc tgctccagga tgaaagccac tcagtgttgg
    13201 caaatgtgca catccattta taaggatgtc aactacagtc agagaacccc tttgtgtttg
    13261 gtcccccccc gtgtcacatg tggaacaggg cccagttggc aagttgtacc aaccaactga
    13321 agggattaca tgcactgccc cgcgaagaag gggcagagat gtcgtagtca ggtttagttc
    13381 gtccggggcg gggatctggc cgcaaatcaa tctaaagtat atatgagtaa acttggtctg
    13441 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    13501 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    13561 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    13621 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    13681 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    13741 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    13801 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    13861 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    13921 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    13981 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    14041 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    14101 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    14161 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    14221 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    14281 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    14341 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    14401 gggttccgcg cacatttccc cgaaaagtgc caccagcttt gctcttagga gtttcctaat
    14461 acatcccaaa ctcaaatata taaagcattt gacttgttct atgccctagg gggcgggggg
    14521 aagctaagcc agcttttttt aacatttaaa atgttaattc cattttaaat gcacagatgt
    14581 ttttatttca taagggtttc aatgtgcatg aatgc
//
