LOCUS       sequence-209221-        8590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ARRET expression contains approximately 130 anti-sense telomere 
            hexanucleotide repeats of TTAGGG and TTGGGG variant sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8590)
  TITLE     telomeric repeat-containing RNA (TERRA and ARRET) plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV UbC6-ARRET
FEATURES             Location/Qualifiers
     source          1..8590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1803..3012
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     2849..2868
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3072..3096
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4023..4047)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4100..4141
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4178..4198)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4183..4512
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4363..4498
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4560..4607
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4626..5024
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5111..5344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5416..5537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5453..5472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5507..5526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5577..5712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5732..5751)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5733..5751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5761..5778)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5761..5777)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5770..5792)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5919..6374
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6006..6025)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6216..6237
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6400..6504
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6505..6573,6574..7365)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6723..6742)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7536..8124
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8025..8044
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8278..8295
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8412..8433
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8448..8453,8454..8471,8472..8478)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8486..8502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8491..8513
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8510..8526
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8510..8526
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8545..8565
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8547..8565
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggat
     1801 ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
     1861 tgccacgtca gacgaagggc gcaggagcgt cctgatcctt ccgcccggac gctcaggaca
     1921 gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta
     1981 ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag
     2041 gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga
     2101 tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg
     2161 tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc
     2221 tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg
     2281 ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc
     2341 gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg
     2401 aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct
     2461 tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc
     2521 tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag
     2581 ttatgcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
     2641 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
     2701 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
     2761 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     2821 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     2881 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     2941 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     3001 tttttgttag acgaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc
     3061 tgcagatatc aacaagtttg tacaaaaaag caggctttaa aggaaccaat tcagtcgatc
     3121 gactggatcc gatgaattcc gaaccctaac cctaacccta accctaaccc taaccctaac
     3181 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3241 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3301 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3361 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3421 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3481 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3541 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3601 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3661 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3721 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3781 cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc taaccctaac
     3841 cctaacccta accctaaccc taaccctaac cccaacccta accctaaccc taacgctaac
     3901 cctaacccca accctaaccc caaccccaac cccaacccca accccaaccg gaattccgcg
     3961 gaattcgagc tcggtacccg gggatcgaca attccacttt ttttaattct cgagatatct
     4021 agacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct
     4081 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     4141 gcgtaccggt tagtaatgag tttggaatta attctgtgga atgtgtgtca gttagggtgt
     4201 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4261 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     4321 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     4381 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     4441 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4501 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     4561 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
     4621 aaaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     4681 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     4741 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     4801 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     4861 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     4921 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     4981 ggattcgtga attgctgccc tctggttatg tgtgggaggg ctaagcacaa ttcgagctcg
     5041 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5101 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     5161 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5221 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5281 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5341 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     5401 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5521 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     5581 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5641 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5701 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     5761 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5821 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     5881 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     5941 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6001 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6061 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6121 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6181 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6241 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6301 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     6361 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6481 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6541 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6601 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6661 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6721 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6781 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6841 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6901 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6961 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7021 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7081 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7141 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7201 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7261 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7321 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7381 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7441 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7501 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7561 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7621 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7681 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7741 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7801 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7861 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7921 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7981 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8041 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8101 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8161 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8221 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8281 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     8341 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     8401 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     8461 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     8521 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     8581 ctgcaagctt
//
