LOCUS       sequence-209222-        3595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ARRET with approximately 130 telomere hexanucleotide repeats of 
            TTAGGG and TTGGGG variant sequences under control of hH1 promoter 
            and terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3595)
  TITLE     telomeric repeat-containing RNA (TERRA and ARRET) plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR hH1-ARRET
FEATURES             Location/Qualifiers
     source          1..3595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(907..1006)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1023..1042)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1024..1042)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1047..1063)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1047..1063)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1165..1184)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1176..1985
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1243..1262)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2132..2720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2621..2640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2874..2891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3050..3077)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3169..3255)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3304..3326
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3318..3335
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3319..3335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3351..3450
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3485..3575
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     3496..3515
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 gatctgatat catcgatgaa ttccgaaccc taaccctaac cctaacccta accctaaccc
       61 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      121 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      181 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      241 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      301 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      361 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      421 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      481 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      541 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      601 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      661 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      721 taaccctaac cctaacccta accctaaccc taaccccaac cctaacccta accctaacgc
      781 taaccctaac cccaacccta accccaaccc caaccccaac cccaacccca accggaattc
      841 cgcggaattc gagctcggta cccggggatc gacaattcca ctttttttaa ttctcgagat
      901 atctagaccc agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
      961 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     1021 tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt
     1081 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     1141 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     1201 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     1261 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     1321 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     1381 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     1441 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
     1501 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     1561 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     1621 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     1681 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     1741 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     1801 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     1861 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     1921 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     1981 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
     2041 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     2101 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2161 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2221 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2281 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2341 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2401 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2461 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2521 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2581 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2641 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2701 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2761 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2821 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2881 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2941 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3001 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     3061 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     3121 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     3181 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     3241 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt
     3301 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga
     3361 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
     3421 taatgccaac tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gatcgactgg
     3481 atccatattt gcatgtcgct atgtgttctg ggaaatcacc ataaacgtga aatgtctttg
     3541 gatttgggaa tcttataagt tctgtatgag accactcgcg gccagtggaa ttgtc
//
