LOCUS       sequence-209223-        3470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ARRET with approximately 130 telomere hexanucleotide repeats of 
            TTAGGG and TTGGGG variant sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3470)
  TITLE     telomeric repeat-containing RNA (TERRA and ARRET) plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR ARRET
FEATURES             Location/Qualifiers
     source          1..3470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(894..993)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1010..1029)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1011..1029)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1034..1050)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1034..1050)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1152..1171)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1163..1972
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1230..1249)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2119..2707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2608..2627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2861..2878
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3037..3064)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3156..3242)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3291..3313
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3305..3322
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3306..3322
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3338..3437
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cgatgaattc cgaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
       61 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      121 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      181 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      241 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      301 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      361 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      421 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      481 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      541 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      601 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      661 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
      721 aaccctaacc ctaaccctaa ccccaaccct aaccctaacc ctaacgctaa ccctaacccc
      781 aaccctaacc ccaaccccaa ccccaacccc aaccccaacc ggaattccgc ggaattcgag
      841 ctcggtaccc ggggatcgac aattccactt tttttaattc tcgagatatc tagacccagc
      901 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
      961 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     1021 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     1081 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     1141 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     1201 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     1261 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     1321 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     1381 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     1441 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     1501 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     1561 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     1621 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     1681 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     1741 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     1801 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     1861 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     1921 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     1981 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
     2041 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     2101 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2161 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2221 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     2281 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2341 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2401 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2461 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     2521 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2581 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2641 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2701 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2761 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     2821 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2881 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     2941 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac
     3001 gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     3061 ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     3121 cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     3181 accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt
     3241 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
     3301 acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact
     3361 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
     3421 gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgat cgactggatc
//
