LOCUS       sequence-209226-        7899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TERRA expression contains approximately 130 telomere hexanucleotide 
            repeats of TTAGGG and TTGGGG variant sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Kreilmeier T, Mejri D, Hauck M, Kleiter M, Holzmann K
  TITLE     Telomere Transcripts Target Telomerase in Human Cancer Cells.
  JOURNAL   Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 
            10.3390/genes7080046.
  PUBMED    27537914
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2016-08-16"; volume: "7(8). pii: genes7080046. 
            doi"; pages: " 10.3390/genes7080046"
COMMENT     Sequence Label: pLV CMV-TERRA
FEATURES             Location/Qualifiers
     source          1..7899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        1816..2119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2274..2294
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2320..2344
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2440..2464
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3385..3409)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3462..3503
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3540..3560)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3545..3875
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3726..3861
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3887..3934
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3953..4327
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     LTR             4420..4653
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4725..4846
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4762..4781)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4816..4835
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4886..5021
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5041..5060)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5042..5060)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5070..5087)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5070..5086)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5079..5101)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5228..5683
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5315..5334)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5525..5546
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5709..5813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5814..5882,5883..6674)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6032..6051)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6845..7433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7334..7353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7587..7604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7721..7742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7757..7762,7763..7780,7781..7787)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7795..7811
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7800..7822
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7819..7835
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7819..7835
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7854..7874
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7856..7874
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
     2341 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag
     2401 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca
     2461 ggctttaaag gaaccaattc agtcgatcga ctggatccga attccgcgga attccggttg
     2521 gggttggggt tggggttggg gttggggtta gggttggggt tagggttagc gttagggtta
     2581 gggttagggt tggggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     2641 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     2701 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     2761 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     2821 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     2881 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     2941 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     3001 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     3061 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     3121 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     3181 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     3241 gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg gttagggtta
     3301 gggttagggt tagggttagg gttcggaatt catcgatgat atcagatcga caattccact
     3361 ttttttaatt ctcgagatat ctagacccag ctttcttgta caaagtggtt gatatccagc
     3421 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
     3481 ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agtttggaat taattctgtg
     3541 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc
     3601 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     3661 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     3721 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3781 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     3841 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccctgttg acaattaatc
     3901 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     3961 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     4021 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     4081 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     4141 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     4201 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     4261 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     4321 ggactgacac gtgctacgag atttaaatgg tacctttaag accaatgact tacaaggcag
     4381 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4441 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     4501 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4561 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4621 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     4681 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     4741 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4801 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     4861 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     4921 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     4981 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     5041 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     5101 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5161 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5221 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5281 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5341 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5401 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5461 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5521 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     5581 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     5641 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     5701 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     5761 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5821 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5881 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5941 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6001 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     6061 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6121 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6181 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6241 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6301 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6361 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6421 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6481 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6541 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6601 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6661 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     6721 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     6781 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6961 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7261 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7321 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7381 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7441 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7501 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7561 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     7621 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     7681 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     7741 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     7801 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     7861 accctcacta aagggaacaa aagctggagc tgcaagctt
//
