LOCUS       sequence-209230-        3464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TERRA with approximately 130 telomere hexanucleotide repeats of 
            TTAGGG and TTGGGG variant sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Kreilmeier T, Mejri D, Hauck M, Kleiter M, Holzmann K
  TITLE     Telomere Transcripts Target Telomerase in Human Cancer Cells.
  JOURNAL   Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 
            10.3390/genes7080046.
  PUBMED    27537914
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2016-08-16"; volume: "7(8). pii: genes7080046. 
            doi"; pages: " 10.3390/genes7080046"
COMMENT     Sequence Label: pENTR TERRA
FEATURES             Location/Qualifiers
     source          1..3464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1589..1688)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1705..1724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1706..1724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1729..1745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1729..1745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1847..1866)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1858..2667
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1925..1944)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2814..3402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3303..3322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttt aaaggaacca attcagtcga tcgactggat ccgaattccg cggaattccg
      721 gttggggttg gggttggggt tggggttggg gttagggttg gggttagggt tagcgttagg
      781 gttagggtta gggttggggt tagggttagg gttagggtta gggttagggt tagggttagg
      841 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
      901 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
      961 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
     1021 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
     1081 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
     1141 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
     1201 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
     1261 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
     1321 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
     1381 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
     1441 gttagggtta gggttagggt tagggttagg gttagggtta gggttagggt tagggttagg
     1501 gttagggtta gggttagggt tagggttcgg aattcatcga tgatatcaga tcgacaattc
     1561 cacttttttt aattctcgag atatctagac ccagctttct tgtacaaagt tggcattata
     1621 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     1681 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     1741 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1801 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1861 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     1921 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     1981 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     2041 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     2101 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     2161 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     2221 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     2281 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     2341 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     2401 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     2461 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     2521 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     2581 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     2641 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     2701 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     2761 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2821 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2881 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2941 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     3001 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3061 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3181 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     3241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
