LOCUS       sequence-209231-        3595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TERRA with approximately 130 telomere hexanucleotide repeats of 
            TTAGGG and TTGGGG variant sequences under control of hH1 promoter 
            and terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Kreilmeier T, Mejri D, Hauck M, Kleiter M, Holzmann K
  TITLE     Telomere Transcripts Target Telomerase in Human Cancer Cells.
  JOURNAL   Genes (Basel). 2016 Aug 16;7(8). pii: genes7080046. doi: 
            10.3390/genes7080046.
  PUBMED    27537914
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            (Basel)."; date: "2016-08-16"; volume: "7(8). pii: genes7080046. 
            doi"; pages: " 10.3390/genes7080046"
COMMENT     Sequence Label: pENTR hH1-TERRA
FEATURES             Location/Qualifiers
     source          1..3595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        703..793
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     714..733
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(1720..1819)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1836..1855)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1837..1855)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1860..1876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1860..1876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1978..1997)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1989..2798
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2056..2075)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2945..3533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3434..3453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttt aaaggaacca attcagtcga tcgactggat ccatatttgc atgtcgctat
      721 gtgttctggg aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc
      781 tgtatgagac cactcgcggc cagtggaatt gtcgatcccc gggtaccgag ctcgaattcc
      841 gcggaattcc ggttggggtt ggggttgggg ttggggttgg ggttagggtt ggggttaggg
      901 ttagcgttag ggttagggtt agggttgggg ttagggttag ggttagggtt agggttaggg
      961 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1021 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1081 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1141 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1201 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1261 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1321 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1381 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1441 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1501 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1561 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     1621 ttagggttag ggttagggtt agggttaggg ttagggttcg gaattcatcg atgatatcag
     1681 atcgacaatt ccactttttt taattctcga gatatctaga cccagctttc ttgtacaaag
     1741 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1801 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     1861 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1921 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1981 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     2041 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     2101 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     2161 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     2221 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2281 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2341 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2401 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2461 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2521 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2581 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2641 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2701 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2761 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     2821 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     2881 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2941 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3001 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3061 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     3121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3301 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg
     3361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
