LOCUS       sequence-209239-        5923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mRIN2 dRHVPS9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Sandri C, Caccavari F, Valdembri D, Camillo C, Veltel S, 
            Santambrogio M, Lanzetti L, Bussolino F, Ivaska J, Serini G
  TITLE     The R-Ras/RIN2/Rab5 complex controls endothelial cell adhesion and 
            morphogenesis via active integrin endocytosis and Rac signaling.
  JOURNAL   Cell Res. 2012 Oct;22(10):1479-501. doi: 10.1038/cr.2012.110. Epub 
            2012 Jul 24.
  PUBMED    22825554
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2012.110"; journalName: "Cell Res"; date: "2012-10"; 
            volume: "22"; issue: "10"; pages: "1479-501"
COMMENT     Sequence Label: HA-mRIN2 dRHVPS9
FEATURES             Location/Qualifiers
     source          1..5923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        133..436
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        437..640
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     591..611
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     637..661
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          776..871
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus (CMV)"
     CDS             892..918
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             898..924
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    2783..2904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2820..2839)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2874..2893
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2911..3366)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3048..3069)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3260..3279
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3393..3497
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3499..3856
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3499..3690
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3522..3542)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3707..3842
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3891..4685
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3891..4685
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3945..3964)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4555..4574
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4873..4892)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4917..4964
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5293..5881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5782..5801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
       61 accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      121 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      181 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      241 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      301 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      361 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      421 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcagtt
      481 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      541 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      601 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
      661 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
      721 cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga gtgacgtaag
      781 taccgcctat agactctata ggcacacccc tttggctctt atgcatgctg acagactaac
      841 agactgttcc tttcctgggt cttttctgca ggcaccgtcg acttaacaat gtacccatac
      901 gatgttccgg attacgctag cctcccagat ctcgacccat tttgcattct cagcatggtg
      961 agacacaagg atggtggcta ctctgaagac aaggatggga agacatgtcc ccgagactct
     1021 ggctatgaca gcctctccaa caggctcagc attctggacc ggctcctgca tacccatccc
     1081 atatggctgc agctgagtct gagtgaggag gaggcagcag aggtcctgca ggcccagcct
     1141 ccagggatct ttttggttcg gaaatcctcc aaaatgcaga agaaagtcct ctcccttcgc
     1201 ctgccctgtg aatttggagc acctctcaaa gagtttacta taaaggaaag cacctacacc
     1261 ttttccctgg aaggctcagg aatcagcttt gcagatttat tccggcttat tgccttctac
     1321 tgcatcagca gggacgtttt gccattcacc ttgaagctgc cttatgccat ttcaacagcc
     1381 aagacggagt ctcagcttga agaactagcc cagctgggac taaatttttg gagctcctca
     1441 gcagacaaca aacccccgaa ctccccacct ccccataggc ctcttccctc agccggcatc
     1501 tgccctgcct ccttgcgtca gctctgcctt ataaatggag tgcattctat aaaaaccagg
     1561 acgccttcgg agctggagtg cagccagacc aacggagcct tgtgttttat taatcctctt
     1621 ttcttgaaag tgcacagcca ggacctcagc acaggcccaa agcggcccag cacaaggact
     1681 cccaacgcga atggcaccga gagacctcgg tcccccccac ccaggccccc gccacccgct
     1741 attaatagtc tgcacacaag ccctgggcta tccaggactg aaccccagac gagcatgcca
     1801 gaaacagtca accatagcaa acatgggaac gtagctctgc ttggaacaaa accaactccc
     1861 atccctccac cccggctgaa gaagcaggct tcttttttgg aagcagaaag cagcgccaag
     1921 accttgactg cccgccggcc cagccggagc tcggagccgg agcccgagct agagctggag
     1981 ttagagatgg gcacagctgg gcacgcaggt ggagccccgc ctagggacgc ccccggggat
     2041 tgcacaaggg cgccgccgcc cggctctgaa tcacaaccac caccgtgcca tggagccagg
     2101 cagaggctga gcgacatgag cctttccact tcctcctccg actcgctgga gttcgaccgg
     2161 agcatgcctc tgtatgggta cgaggccgac accaccagca gcctggagga ctacgaaggg
     2221 gagagtgacc aagagaccat ggcacctccc atcaagtcca aaaagaagag gaacagctct
     2281 tttgtgctgc ccaagcttag aaccaccaac cgaactatcc cctccgttga cgactttcag
     2341 aattacctcc gagttgcctt ccaagaggtc aacagcggat gcactgggaa gaccctcctt
     2401 gtgagaccgt acatcaccac agaggatgtg tgtcagctct gtgctgagaa gtttaaggtg
     2461 gaggaccctg aagagtacag cctgtttctc tttgttgatg agacatggca acaactggca
     2521 gaggacactt atcctcagaa gatcaaggct gaactgcaca gccggcctca gccccacatc
     2581 ttccactttg tctacaaacg aatcaagagt gatccttacg gggtgatttt ccagaatgga
     2641 gaagacctca ctccctcata ggcggccgcg actctagatc ataatcagcc ataccacatt
     2701 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     2761 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     2821 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2881 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     2941 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3001 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3061 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3121 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3181 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3241 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3301 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3361 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3421 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3481 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     3541 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3601 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3661 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     3721 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3781 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     3841 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     3901 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     3961 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4021 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     4081 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4141 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4201 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4261 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4321 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4381 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     4441 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4501 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4561 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4621 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4681 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     4741 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     4801 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     4861 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     4921 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     4981 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     5041 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     5101 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     5161 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5221 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5281 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5341 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5401 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5461 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5521 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5581 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5641 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5701 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5761 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5821 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5881 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgc
//
