LOCUS       sequence-209242-        6769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of mRIN2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Sandri C, Caccavari F, Valdembri D, Camillo C, Veltel S, 
            Santambrogio M, Lanzetti L, Bussolino F, Ivaska J, Serini G
  TITLE     The R-Ras/RIN2/Rab5 complex controls endothelial cell adhesion and 
            morphogenesis via active integrin endocytosis and Rac signaling.
  JOURNAL   Cell Res. 2012 Oct;22(10):1479-501. doi: 10.1038/cr.2012.110. Epub 
            2012 Jul 24.
  PUBMED    22825554
REFERENCE   2  (bases 1 to 6769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/cr.2012.110"; journalName: "Cell Res"; date: "2012-10"; 
            volume: "22"; issue: "10"; pages: "1479-501"
COMMENT     Sequence Label: HA-mRIN2
FEATURES             Location/Qualifiers
     source          1..6769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     565..589
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          704..799
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus (CMV)"
     CDS             820..846
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             826..852
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    3557..3678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3594..3613)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3648..3667
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3685..4140)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3822..3843)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4034..4053
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4167..4271
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4273..4630
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4273..4464
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4296..4316)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4481..4616
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4665..5459
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4665..5459
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4719..4738)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5329..5348
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5647..5666)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5691..5738
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6067..6655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6556..6575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcagtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      601 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
      661 ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag
      721 actctatagg cacacccctt tggctcttat gcatgctgac agactaacag actgttcctt
      781 tcctgggtct tttctgcagg caccgtcgac ttaacaatgt acccatacga tgttccggat
      841 tacgctagcc tcccagatct cgacccattt tgcattctca gcatggtgag acacaaggat
      901 ggtggctact ctgaagacaa ggatgggaag acatgtcccc gagactctgg ctatgacagc
      961 ctctccaaca ggctcagcat tctggaccgg ctcctgcata cccatcccat atggctgcag
     1021 ctgagtctga gtgaggagga ggcagcagag gtcctgcagg cccagcctcc agggatcttt
     1081 ttggttcgga aatcctccaa aatgcagaag aaagtcctct cccttcgcct gccctgtgaa
     1141 tttggagcac ctctcaaaga gtttactata aaggaaagca cctacacctt ttccctggaa
     1201 ggctcaggaa tcagctttgc agatttattc cggcttattg ccttctactg catcagcagg
     1261 gacgttttgc cattcacctt gaagctgcct tatgccattt caacagccaa gacggagtct
     1321 cagcttgaag aactagccca gctgggacta aatttttgga gctcctcagc agacaacaaa
     1381 cccccgaact ccccacctcc ccataggcct cttccctcag ccggcatctg ccctgcctcc
     1441 ttgcgtcagc tctgccttat aaatggagtg cattctataa aaaccaggac gccttcggag
     1501 ctggagtgca gccagaccaa cggagccttg tgttttatta atcctctttt cttgaaagtg
     1561 cacagccagg acctcagcac aggcccaaag cggcccagca caaggactcc caacgcgaat
     1621 ggcaccgaga gacctcggtc ccccccaccc aggcccccgc cacccgctat taatagtctg
     1681 cacacaagcc ctgggctatc caggactgaa ccccagacga gcatgccaga aacagtcaac
     1741 catagcaaac atgggaacgt agctctgctt ggaacaaaac caactcccat ccctccaccc
     1801 cggctgaaga agcaggcttc ttttttggaa gcagaaagca gcgccaagac cttgactgcc
     1861 cgccggccca gccggagctc ggagccggag cccgagctag agctggagtt agagatgggc
     1921 acagctgggc acgcaggtgg agccccgcct agggacgccc ccggggattg cacaagggcg
     1981 ccgccgcccg gctctgaatc acaaccacca ccgtgccatg gagccaggca gaggctgagc
     2041 gacatgagcc tttccacttc ctcctccgac tcgctggagt tcgaccggag catgcctctg
     2101 tatgggtacg aggccgacac caccagcagc ctggaggact acgaagggga gagtgaccaa
     2161 gagaccatgg cacctcccat caagtccaaa aagaagagga acagctcttt tgtgctgccc
     2221 aagcttgtca agtcccagtt gcgaaagatg agcggggtgt tcagctcctt catgacccct
     2281 gagaagcgga tggtccgcag gatcgccgag ctgtcccggg acaaatgcac ctattttggg
     2341 tgcttggtgc aggactacgt gagcttcctg aaggagaaca aggagtgcca tgtgtccagc
     2401 accgatatgc tgcagaccat ccggcagttt atgacccagg tcaagaacta tttgtctcag
     2461 agctctgaac tggacccccc tattgagtca ctgatcccag aagaccaaat tgatgtggtg
     2521 ttggagaagg ccatgcacaa atgtatcttg aagcccctca agggccatgt ggaggccatg
     2581 ctgaaggact tccacacggc tgacggctcg tggaaacaac tcaaggaaaa cctacaactc
     2641 gtgcggcaga ggaacccaca ggagctgggg gtctttgccc caacccctga cttgatggag
     2701 ctggagaaaa tcaagcttaa attcatgacc atgcagaaga tgtattcgcc agaaaagaaa
     2761 gtcatgcttt tgctacgggt ctgtaagctc atctacactg tcatggagaa caactcaggg
     2821 aggatgtacg gtgcagacga cttcttgcca gtcctgacct atgtcatagc tcagtgtgac
     2881 atgcttgaac tggacacgga aatcgagtac atgatggaac tcctggaccc atcactgctg
     2941 cacggagaag gaggctatta cttgaccagt gcctatgggg cgctatctct gataaagaat
     3001 ttccaagaag aacaggctgc gagactgctc agctccgaag ccagggacac gctgaggcag
     3061 tggcacaagc ggagaaccac caaccgaact atcccctccg ttgacgactt tcagaattac
     3121 ctccgagttg ccttccaaga ggtcaacagc ggatgcactg ggaagaccct ccttgtgaga
     3181 ccgtacatca ccacagagga tgtgtgtcag ctctgtgctg agaagtttaa ggtggaggac
     3241 cctgaagagt acagcctgtt tctctttgtt gatgagacat ggcaacaact ggcagaggac
     3301 acttatcctc agaagatcaa ggctgaactg cacagccggc ctcagcccca catcttccac
     3361 tttgtctaca aacgaatcaa gagtgatcct tacggggtga ttttccagaa tggagaagac
     3421 ctcactccct cataggcggc cgcgactcta gatcataatc agccatacca catttgtaga
     3481 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     3541 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3601 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3661 actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta aaattcgcgt
     3721 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     3781 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     3841 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     3901 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     3961 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     4021 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     4081 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     4141 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4201 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4261 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     4321 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4381 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     4441 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     4501 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4561 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4621 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     4681 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     4741 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     4801 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     4861 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     4921 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     4981 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     5041 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     5101 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     5161 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     5221 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     5281 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     5341 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     5401 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     5461 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     5521 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     5581 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     5641 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     5701 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     5761 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     5821 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     5881 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     5941 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6001 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6061 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6121 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6181 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6241 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6301 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6361 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6421 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6481 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6541 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6601 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6661 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6721 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
