LOCUS       sequence-209257-        5670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-CathespinB-6
FEATURES             Location/Qualifiers
     source          1..5670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        119..422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        423..626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     577..597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1714..2394
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2516..2637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2553..2572)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2607..2626
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2644..3099)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2781..2802)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2993..3012
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3126..3230
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3232..3589
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3232..3423
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3255..3275)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3440..3575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3624..4418
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3624..4418
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3678..3697)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4288..4307
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4606..4625)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4650..4697
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5026..5614
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5515..5534
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
       61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
      601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      661 ggactcagat ctcgagctca agcttcgaat tccaggatgt ggtggtcctt gatccctctc
      721 tcttgcctgc tggcactgac cagtgcccat gacaagcctt cctttcaccc actgtcggac
      781 gacatgatta actatatcaa caaacagaat acaacatggc aggctggacg caacttctac
      841 aatgttgaca taagctatct gaagaagctg tgtggaactg tcctgggtgg acccaagctg
      901 ccggaaaggg ttgggttcag cgaggacata aatctacctg aatcctttga tgcacgggaa
      961 cagtggtcca attgcccgac catcgcacag atcagagacc aggggtcctg tggctcttgt
     1021 tgggcatttg gggcagtgga agccatgtct gaccgaatct gcattcacac caatggccga
     1081 gtcaatgtgg aggtgtctgc tgaggacctg cttacctgct gtggtatcca gtgtggggat
     1141 ggctgtaatg gtggctatcc ctctggagca tggaacttct ggactagaaa aggcctggtt
     1201 tctggtggag tatacaattc tcatataggc tgcttaccct acaccatccc tccctgtgaa
     1261 caccatgtca atggctcccg tcccccatgc actggagaag gagatactcc caagtgcaac
     1321 aagatgtgtg aggctggcta ctccacatcc tacaaggaag ataagcacta tgggtacact
     1381 tcctacagtg tgtctgacag cgagaaggag atcatggcgg aaatctacaa aaatggccca
     1441 gtggagggtg cttttactgt gttttctgac ttcttgactt acaaatcagg cgtatacaag
     1501 catgaagccg gtgatgtgat gggaggccat gccatccgca ttctgggctg gggaatagag
     1561 aatggagtac cctactggct ggtagcaaac tcctggaacg ttgactgggg tgataatggt
     1621 ttctttaaaa tcctcagagg agagaaccac tgtggaattg aatcagaaat cgtggctgga
     1681 atcccacgca ctcaggatcc accggtcgcc accatgagtg cgattaagcc agacatgaag
     1741 atcaaactcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga cggagatggt
     1801 acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga gggcggacct
     1861 ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag ggtattcgcc
     1921 aaatatccag acaacataca agactatttt aagcagtcgt ttcctaaggg gtattcgtgg
     1981 gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga cataacaatg
     2041 gaaggggaca ctttctataa taaagttcga ttttatggta ccaactttcc cgccaatggt
     2101 ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat gtatgtgcgt
     2161 gatggagtgc tgacgggtga tattcatatg gctttgttgc ttgaaggaaa tgcccattac
     2221 cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt accaggctac
     2281 cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa caaggttaag
     2341 ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg ataagcggcc
     2401 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     2461 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     2521 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     2581 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     2641 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     2701 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     2761 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     2821 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     2881 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     2941 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     3001 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     3061 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     3121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     3181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     3241 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     3301 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     3361 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     3421 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3481 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3541 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     3601 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     3661 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     3721 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     3781 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     3841 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     3901 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     3961 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4021 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4081 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4141 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     4201 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     4261 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     4321 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     4381 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     4441 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     4501 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     4561 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     4621 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     4681 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     4741 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     4801 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     4861 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4921 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4981 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5041 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5101 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5161 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5221 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     5281 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     5341 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     5401 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5461 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5521 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5581 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5641 gttcctggcc ttttgctggc cttttgctca
//
